Surface differentiation of ferritin and apoferritin with atomic force microscopic techniques

2012 ◽  
Vol 94 ◽  
pp. 231-235 ◽  
Author(s):  
Ru-Hung Ho ◽  
Yu-Hung Chen ◽  
Chong Mou Wang
Keyword(s):  
Atomic Force ◽  
Microscopic Techniques ◽  
Surface Differentiation

Comparative Study of Nanoscale Surface Structures of Calcite Microcrystals Using FE-SEM, AFM, and TEM

2006 ◽  
Vol 12 (4) ◽  
pp. 302-310 ◽  
Author(s):  
Yung-Ching Chien ◽  
Alfonso Mucci ◽  
Jeanne Paquette ◽  
S. Kelly Sears ◽  
Hojatollah Vali
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Quantitative Information ◽  
Surface Structures ◽  
Surface Features ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Micrometer Size ◽  
Microscopic Techniques

The bulk morphology and surface features that developed upon precipitation on micrometer-size calcite powders and millimeter-size cleavage fragments were imaged by three different microscopic techniques: field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) of Pt-C replicas, and atomic force microscopy (AFM). Each technique can resolve some nanoscale surface features, but they offer different ranges of magnification and dimensional resolutions. Because sample preparation and imaging is not constrained by crystal orientation, FE-SEM and TEM of Pt-C replicas are best suited to image the overall morphology of microcrystals. However, owing to the decoration effect of Pt-C on the crystal faces, TEM of Pt-C replicas is superior at resolving nanoscale surface structures, including the development of new faces and the different microtopography among nonequivalent faces in microcrystals, which cannot be revealed by FE-SEM. In conjunction with SEM, Pt-C replica provides the evidence that crystals grow in diverse and face-specific modes. The TEM imaging of Pt-C replicas has nanoscale resolution comparable to AFM. AFM yielded quantitative information (e.g., crystallographic orientation and height of steps) of microtopographic features. In contrast to Pt-C replicas and SEM providing three-dimensional images of the crystals, AFM can only image one individual cleavage or flat surface at a time.

scholarly journals Scanning Electron Microscopy and Atomic Force Microscopy: Topographic and Dynamical Surface Studies of Blends, Composites, and Hybrid Functional Materials for Sustainable Future

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Joanna Rydz ◽  
Alena Šišková ◽  
Anita Andicsová Eckstein
Keyword(s):  
Functional Materials ◽  
Degradation Process ◽  
Hybrid Functional ◽  
Surface Studies ◽  
Aliphatic Polyesters ◽  
Force Microscopy ◽  
Atomic Force ◽  
Materials Used ◽  
Physical And Chemical ◽  
Microscopic Techniques

Microscopic techniques are often used in material science, enabling the assessment of the morphology, composition, physical properties, and dynamic behaviour of materials. The review focuses on the topographic and dynamical surface studies of (bio)degradable polymers, in particular aliphatic polyesters, the most promising ones. The (bio)degradation process promotes physical and chemical changes in material properties that can be characterised by microscopic techniques. These changes occurring both under controlled conditions as well as in the processing stage or during use indicate morphological and structural transformations resulting from the deterioration of the material and have a significant impact on the characteristic of materials used in many applications, for example, for use as packaging.

A Blueprint for the Synthesis and Characterisation of Thin Graphene Oxide with Controlled Lateral Dimensions for Biomedicine

2018 ◽  
Author(s):  
Artur Rodrigues ◽  
Leon Newman ◽  
Neus Lozano ◽  
Sourav Prasanna Mukherjee ◽  
Bengt Fadeel ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Zeta Potential ◽  
Hazard Assessment ◽  
Atomic Force ◽  
Microscopic Techniques

We report the reproducible production of graphene oxide flakes of controlled lateral dimensions under endotoxin free conditions for biomedical and hazard assessment investigations. In addition, we report the thorough characterisation of those materials, using optical, atomic force, electron microcopies; fluorescence, UV/Vis and Raman spectroscopies; Zeta potential, FTIR, TGA, and XPS. We identified that a combination of microscopic techniques are needed to fully assess the lateral dimensions of GO flakes. <br>

In Vitro Studies of Microtubule Structures Using the Mac Mode AFM

1999 ◽  
Vol 5 (S2) ◽  
pp. 1006-1007
Author(s):  
J. Zhu ◽  
J. Hartman ◽  
R. Case ◽  
S. Rice ◽  
R. Vale
Keyword(s):  
Structural Unit ◽  
Electron Microscopic ◽  
Physiological Conditions ◽  
Atomic Force ◽  
Chromosome Separation ◽  
Cytoplasmic Microtubules ◽  
Dynamic Structures ◽  
Microscopic Techniques ◽  
Β Tubulin

Microtubules are long, hollow, stiff polymers that extend throughout the cytoplasm. They are involved in such diverse functions as governing the location of membrane-bounded organelles and chromosome separation during mitosis. The basic structural unit is tubulin, which is a heterodimer consisting of two closely related and tightly linked globular polypeptides called α and β-tubulin. Alternating α and β tubulin subunits form protofilaments, 13 of which bundle around a central core to form microtubule. The detailed structures of cytoplasmic microtubules have been studied extensively using various electron microscopic techniques. As microtubules are dynamic structures in constant transitions between growing and shrinking phases, it would be extremely interesting to investigate the structural organization of the subunit tubulin molecules in a buffer close to physiological conditions. With its high resolution and ability to image in fluid, atomic force microscope (AFM) makes it possible to study the biological structures in a native environment.

Development of Porosity Measurement Method Based on Modern Microscopic Techniques

2015 ◽  
Vol 240 ◽  
pp. 87-93
Author(s):  
Ewelina Małek ◽  
Tadeusz Niezgoda ◽  
Danuta Miedzińska
Keyword(s):  
Porous Structure ◽  
Innovative Technology ◽  
Rock Fracturing ◽  
Gas Recovery ◽  
Atomic Force ◽  
University Of Technology ◽  
Fracturing Process ◽  
Productivity Increase ◽  
Gaseous Hydrocarbons ◽  
Microscopic Techniques

The aim of the research, presented in the paper, is to show and to assess the porosity structure in accordance to the dimensions of carbon dioxide particle. The characteristic surface morphology of the sample and the visualisation of the coal porous structure have been obtained using the atomic force microscope (AFM). The presented study of the coal microstructure is a part of the concepts of the project which aim is to develop the guidelines for design of the innovative technology of shale gas recovery with the use of liquid CO2. The technology will be based on Military University of Technology invention which considers gaseous hydrocarbons recovery from at least two levels of lateral wellbores with the use of supercritical CO2, what will result with wellbore productivity increase, because CO2 will cause desorption of CH4 from the porous structure of shale rock and the thermodynamic transformation of CO2 in the reservoir will help the rock fracturing. The heat energy added for the fracturing process will be taken from the surrounding rock mass.

A Blueprint for the Synthesis and Characterisation of Thin Graphene Oxide with Controlled Lateral Dimensions for Biomedicine

2018 ◽  
Author(s):  
Artur Rodrigues ◽  
Leon Newman ◽  
Neus Lozano ◽  
Sourav Prasanna Mukherjee ◽  
Bengt Fadeel ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Zeta Potential ◽  
Hazard Assessment ◽  
Atomic Force ◽  
Microscopic Techniques

We report the reproducible production of graphene oxide flakes of controlled lateral dimensions under endotoxin free conditions for biomedical and hazard assessment investigations. In addition, we report the thorough characterisation of those materials, using optical, atomic force, electron microcopies; fluorescence, UV/Vis and Raman spectroscopies; Zeta potential, FTIR, TGA, and XPS. We identified that a combination of microscopic techniques are needed to fully assess the lateral dimensions of GO flakes. <br>

scholarly journals Compatibilization and Characterization of Polylactide and Biopolyethylene Binary Blends by Non-Reactive and Reactive Compatibilization Approaches

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1344 ◽  
Author(s):  
Jose M. Ferri ◽  
Daniel Garcia-Garcia ◽  
Emilio Rayón ◽  
Maria D. Samper ◽  
Rafael Balart
Keyword(s):  
Electron Microscopy ◽  
Vinyl Acetate ◽  
Charpy Test ◽  
Binary Blends ◽  
Reactive Compatibilization ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Field Emission Electron Microscopy ◽  
Microscopic Techniques

In this study, different compatibilizing agents were used to analyze their influence on immiscible blends of polylactide (PLA) and biobased high-density polyethylene (bioPE) 80/20 (wt/wt). The compatibilizing agents used were polyethylene vinyl acetate (EVA) with a content of 33% of vinyl acetate, polyvinyl alcohol (PVA), and dicumyl peroxide (DPC). The influence of each compatibilizing agent on the mechanical, thermal, and microstructural properties of the PLA-bioPE blend was studied using different microscopic techniques (i.e., field emission electron microscopy (FESEM), transmission electron microscopy (TEM), and atomic force microscopy with PeakForce quantitative nanomechanical mapping (AFM-QNM)). Compatibilized PLA-bioPE blends showed an improvement in the ductile properties, with EVA being the compatibilizer that provided the highest elongation at break and the highest impact-absorbed energy (Charpy test). In addition, it was observed by means of the different microscopic techniques that the typical droplet-like structure is maintained, but the use of compatibilizers decreases the dimensions of the dispersed droplets, leading to improved interfacial adhesion, being more pronounced in the case of the EVA compatibilizer. Furthermore, the incorporation of the compatibilizers caused a very marked decrease in the crystallinity of the immiscible PLA-bioPE blend.

scholarly journals Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fabian Christopher Herrmann
Keyword(s):  
Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Easy Access ◽  
Experimental Protocol ◽  
Force Microscopy ◽  
The Past ◽  
Atomic Force ◽  
Internal Morphology ◽  
Insight Into ◽  
Microscopic Techniques

AbstractAs a topographical technique, Atomic Force Microscopy (AFM) needs to establish direct interactions between a given sample and the measurement probe in order to create imaging information. The elucidation of internal features of organisms, tissues and cells by AFM has therefore been a challenging process in the past. To overcome this hindrance, simple and fast embedding, sectioning and dehydration techniques are presented, allowing the easy access to the internal morphology of virtually any organism, tissue or cell by AFM. The study at hand shows the applicability of the proposed protocol to exemplary biological samples, the resolution currently allowed by the approach as well as advantages and shortcomings compared to classical ultrastructural microscopic techniques like electron microscopy. The presented cheap, facile, fast and non-toxic experimental protocol might introduce AFM as a universal tool for the elucidation of internal ultrastructural detail of virtually any given organism, tissue or cell.

scholarly journals Photoluminescence of MoS2Prepared by Effective Grinding-Assisted Sonication Exfoliation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Jing-Yuan Wu ◽  
Meng-Na Lin ◽  
Long-De Wang ◽  
Tong Zhang
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Sonication Time ◽  
Final Solution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Microscopic Techniques

Exfoliation of bulk molybdenum disulfide (MoS2) using sonication in appropriate solvent is a promising route to large-scale preparation of few-layered or monolayered crystals. Grinding-assisted sonication exfoliation was used for preparing monolayered MoS2nanosheets from natural mineral molybdenite. By controlling the sonication time, larger crystallites could be further exfoliated to smaller as well as thinner nanosheets without damaging their structures. The concentration of 1.6 mg mL−1of final solution could be achieved. Several microscopic techniques like scanning electron microscopy, transmission electron microscopy, and atomic force microscopy were employed to evaluate the exfoliation results. Strong photoluminescence with the peak centered at 440 nm was also observed in the resulting dispersion which included several small lateral-sized (~3 nm) nanostructures.

scholarly journals The Luminescence of 1,8-Diazafluoren-9-One/Titanium Dioxide Composite Thin Films for Optical Application

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 3014
Author(s):  
Aneta Lewkowicz ◽  
Robert Bogdanowicz ◽  
Piotr Bojarski ◽  
Mattia Pierpaoli ◽  
Ignacy Gryczyński ◽  
...  
Keyword(s):  
Amino Acids ◽  
Titanium Dioxide ◽  
Detection Methods ◽  
Label Free ◽  
Force Microscopy ◽  
Transparent Substrate ◽  
Atomic Force ◽  
Potential Marker ◽  
Fluorescent Products ◽  
Microscopic Techniques

The investigation of innovative label-free α-amino acids detection methods represents a crucial step for the early diagnosis of several diseases. While 1,8-diazafluoren-9-one (DFO) is known in forensic application because of the fluorescent products by reacting with the amino acids present in the papillary exudate, its application for diagnostic purposes has not been fully investigated. The stabilization of DFO over a transparent substrate allows its complexation with biomolecules for the detection of α-amino acids. In this study, DFO was immobilized into a titanium dioxide (TiO2) matrix for the fluorescence detection of glycine, as a target α-amino acid (a potential marker of the urogenital tract cancers). The DFO/TiO2 composite was characterized by atomic force microscopy, spectroscopic ellipsometry, fluorescence spectroscopy and fluorescence microscopy. The performed fluorescent studies indicate spectacular formation of aggregates at higher concentration. The measurements performed using various fluorescence and microscopic techniques together with the suitable analysis show that the aggregates are able to emit short-lived fluorescence.

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