Stability under flow conditions of trypsin immobilized onto poly(vinyl amine) functionalized silica microparticles

Author(s):  
Florin Bucatariu ◽  
Frank Simon ◽  
Cornelia Bellmann ◽  
Gheorghe Fundueanu ◽  
Ecaterina Stela Dragan
2020 ◽  
Vol 37 (12) ◽  
pp. 2317-2325
Author(s):  
Seong Bin Jo ◽  
Ho Jin Chae ◽  
Tae Young Kim ◽  
Jeom-In Baek ◽  
Dhanusuraman Ragupathy ◽  
...  

Author(s):  
Gabrielle Rey ◽  
Stephanie L. Vivod ◽  
Saranshu Singla ◽  
Theresa Benyo ◽  
Jaelynne King ◽  
...  

Nanoscale ◽  
2012 ◽  
Vol 4 (15) ◽  
pp. 4680 ◽  
Author(s):  
Filipe Natalio ◽  
Anubha Kashyap ◽  
Steffen Lorenz ◽  
Hannes Kerschbaumer ◽  
Michael Dietzsch ◽  
...  

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4560 ◽  
Author(s):  
Park ◽  
Kim ◽  
Kim ◽  
Pyun ◽  
Sung

A portable urea sensor for use in fast flow conditions was fabricated using porous polytetrafluoroethylene (PTFE) membranes coated with amine-functionalized parylene, parylene-A, by vapor deposition. The urea-hydrolyzing enzyme urease was immobilized on the parylene-A-coated PTFE membranes using glutaraldehyde. The urease-immobilized membranes were assembled in a polydimethylsiloxane (PDMS) fluidic chamber, and a screen-printed carbon three-electrode system was used for electrochemical measurements. The success of urease immobilization was confirmed using scanning electron microscopy, and fourier-transform infrared spectroscopy. The optimum concentration of urease for immobilization on the parylene-A-coated PTFE membranes was determined to be 48 mg/mL, and the optimum number of membranes in the PDMS chamber was found to be eight. Using these optimized conditions, we fabricated the urea biosensor and monitored urea samples under various flow rates ranging from 0.5 to 10 mL/min in the flow condition using chronoamperometry. To test the applicability of the sensor for physiological samples, we used it for monitoring urea concentration in the waste peritoneal dialysate of a patient with chronic renal failure, at a flow rate of 0.5 mL/min. This developed urea biosensor is considered applicable for (portable) applications, such as artificial kidney systems and portable dialysis systems.


2019 ◽  
Vol 58 (42) ◽  
pp. 19465-19474
Author(s):  
Yiying Zhao ◽  
Jizhi Zhou ◽  
Lili Fan ◽  
Lin Chen ◽  
Li Li ◽  
...  

2018 ◽  
Vol 5 (10) ◽  
pp. 21413-21420
Author(s):  
M.N. Chandraprabha ◽  
K. Samrat ◽  
N. Ahalya ◽  
B. Sharada ◽  
Kavya Venkat ◽  
...  

2015 ◽  
Vol 44 (3) ◽  
pp. 1303-1316 ◽  
Author(s):  
R. K. Sharma ◽  
Sriparna Dutta ◽  
Shivani Sharma

A novel and magnetically recoverable nanocatalytic system consisting of a magnetic silica based copper complex has been fabricated and applied in the synthesis of carbamates via C–H activation of formamides.


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