Antibacterial, anti-biofilm and angiogenic calcium sulfate-nano MgO composite bone void fillers for inhibiting Staphylococcus aureus infections

2020 ◽  
Vol 39 ◽  
pp. 100332
Author(s):  
Jishita Ravoor ◽  
Sivashanmugam Amirthalingam ◽  
Thadi Mohan ◽  
Jayakumar Rangasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Calcium Sulfate ◽  
Bone Void ◽  
Composite Bone

In Vitro Antimicrobial Activity of Calcium Sulfate and Hydroxyapatite (Cerament Bone Void Filler) Discs Using Heat-Sensitive and Non–Heat-sensitive Antibiotics Against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2011 ◽  
Vol 101 (2) ◽  
pp. 146-152 ◽  
Author(s):  
Jeffrey C. Karr ◽  
Joseph Lauretta ◽  
Georgia Keriazes
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Calcium Sulfate ◽  
Bone Void Filler ◽  
Zones Of Inhibition ◽  
Standard Set ◽  
Bone Void ◽  
Carrier Systems ◽  
Carrier Vehicle

Background: Several absorbable and nonabsorbable antibiotic carrier systems are available in the adjunctive surgical management of osteomyelitis of the foot, ankle, and lower leg. These carrier systems have significant limitations regarding which antibiotics can be successfully incorporated into the carrier vehicle. The calcium sulfate and hydroxyapatite Cerament Bone Void Filler is a biocompatible, absorbable ceramic bone void filler that can successfully deliver multiple heat-stable and heat-unstable antibiotics that have not been generally used before with antibiotic beads in treating musculoskeletal infections. Methods: Cerament Bone Void Filler discs with the antibiotics rifampin, vancomycin, tobramycin, cefazolin, cefepime hydrochloride, vancomycin-tobramycin, piperacillin-tazobactam, ceftazidime, and ticarcillin-clavulanate were tested in vitro against methicillin-resistant Staphylococcus aureus. Results: The zones of inhibition for the Cerament Bone Void Filler antibiotic discs plated against Staphylococcus aureus obtained were 33% to 222% greater than the minimum zones of inhibition breakpoints for bacteria susceptibility as defined by the standard set by the Clinical and Laboratory Standards Institute. Cerament Bone Void Filler discs with the antibiotics plated against Pseudomonas aeruginosa produced zones of inhibition of 93% to 200% greater than the minimum zones of inhibition breakpoints for bacteria susceptibility as defined by the standard set by the Clinical and Laboratory Standards Institute. Conclusions: The calcium sulfate and hydroxyapatite Cerament Bone Void Filler was an excellent carrier vehicle for multiple antibiotics creating in vitro significant zones of inhibition, thus demonstrating susceptibility against Staphylococcus aureus and Pseudomonas aeruginosa, which holds tremendous promise in treating osteomyeilits. (J Am Podiatr Med Assoc 101(2): 146–152, 2011)

scholarly journals Comparison of Borate Bioactive Glass and Calcium Sulfate as Implants for the Local Delivery of Teicoplanin in the Treatment of Methicillin-Resistant Staphylococcus aureus-Induced Osteomyelitis in a Rabbit Model

2015 ◽  
Vol 59 (12) ◽  
pp. 7571-7580 ◽  
Author(s):  
Wei-Tao Jia ◽  
Qiang Fu ◽  
Wen-Hai Huang ◽  
Chang-Qing Zhang ◽  
Mohamed N. Rahaman
Keyword(s):  
Staphylococcus Aureus ◽  
Bioactive Glass ◽  
Calcium Sulfate ◽  
Rabbit Model ◽  
Local Delivery ◽  
Methicillin Resistant ◽  
Content Type ◽  
Borate Bioactive Glass ◽  
Phosphate Buffered Saline

ABSTRACTThere is growing interest in biomaterials that can cure bone infection and also regenerate bone. In this study, two groups of implants composed of 10% (wt/wt) teicoplanin (TEC)-loaded borate bioactive glass (designated TBG) or calcium sulfate (TCS) were created and evaluated for their ability to release TECin vitroand to cure methicillin-resistantStaphylococcus aureus(MRSA)-induced osteomyelitis in a rabbit model. When immersed in phosphate-buffered saline (PBS), both groups of implants provided a sustained release of TEC at a therapeutic level for up to 3 to 4 weeks while they were gradually degraded and converted to hydroxyapatite. The TBG implants showed a longer duration of TEC release and better retention of strength as a function of immersion time in PBS. Infected rabbit tibiae were treated by debridement, followed by implantation of TBG or TCS pellets or intravenous injection with TEC, or were left untreated. Evaluation at 6 weeks postimplantation showed that the animals implanted with TBG or TCS pellets had significantly lower radiological and histological scores, lower rates of MRSA-positive cultures, and lower bacterial loads than those preoperatively and those of animals treated intravenously. The level of bone regeneration was also higher in the defects treated with the TBG pellets. The results showed that local TEC delivery was more effective than intravenous administration for the treatment of MRSA-induced osteomyelitis. Borate glass has the advantages of better mechanical strength, more desirable kinetics of release of TEC, and a higher osteogenic capacity and thus could be an effective alternative to calcium sulfate for local delivery of TEC.

Assessment of α-calcium sulfate hemihydrate/nanocellulose composite bone graft material for bone healing in a rabbit femoral condyle model

2021 ◽  
Vol 11 (9) ◽  
pp. 1497-1504
Author(s):  
Jinlong Liu ◽  
Yicai Zhang ◽  
Lin Qiu ◽  
Yujuan Zhang ◽  
Bin Gao
Keyword(s):  
Bone Graft ◽  
Calcium Sulfate ◽  
Biological Activities ◽  
Femoral Condyle ◽  
Three Dimensional ◽  
Graft Material ◽  
Bone Graft Material ◽  
Calcium Sulfate Hemihydrate ◽  
Composite Bone

The material properties of nanocellulose (NC) can effectively enhance the structural stability of composite materials. However, the research related to NC/α-calcium sulfate hemihydrate (CSH) composites is largely lacking. In this paper, we explore the combination of these two materials and determine their elaborate biological activities in vivo. Using α-CSH as the matrix, the composite bone graft materials were produced according to different proportions of NC. Then the mechanical strength of the composite bone graft was measured, and the results were analyzed by X-ray diffraction and scanning electron microscopy (SEM). To conduct the material in vivo evaluation, 0% (CN0) and 0.75% (CN0.75) NC/α-CSH composite bone graft materials were implanted into a femoral condyle defect model. The results indicated that NC could significantly enhance the mechanical properties of α-CSH. The SEM analysis indicated that the NC shuttled between the crystal gaps and formed a three-dimensional network structure, which was firmly combined with the crystal structure. Meanwhile, the CN0.75 scaffold remained at 12 weeks postoperation, which provided a long-term framework for new bone formation. Overall, our findings demonstrate that, with a 0.75% NC/α-CSH composite demonstrating good potential as a bone graft material for clinical bone grafting.

scholarly journals Reconstruction of anterior frontal wall and contours defects using bone substitute

2021 ◽  
pp. 34
Author(s):  
Keyword(s):  
Bone Substitute ◽  
Calcium Sulfate ◽  
Donor Site ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Bone Void Filler ◽  
Custom Made ◽  
Cranial Implant ◽  
Bone Void ◽  
Orbital Area

Aim: The aim of this article is to report on the safety and long-term efficacy of Cerament® BoneVoid Filler bone substitute for repairing craniofacial bone defects. Post-traumatic cranioplasty is a complex and challenging procedure for all maxillo-craniofacial surgeons and neurosurgeons, especially when repairing large areas. The standard criterion for repairing small cranial defects is the use autogenous bone from the iliac crest or split calvarial grafts. Autogenous grafts may result in donor-site morbidity, increased surgical time, reabsorption, blood loss, and longer recovery time . Alloplastic materials used for bone repair, such as methyl methacrylate, hydroxyapatite, titanium, or porous polyethylene, are expected to have optimal properties, including easy adaptation, biocompatibility, ingrowth of new tissue, stability of shape, and low rate of reabsorption. A cranial implant should be easily shaped and positioned, allowing easy tissue growth. In very wide cranium defects the new technology is a custom made cranial implant constructed three-dimensionally with different types of materials. However, this procedure is very expensive with various infection rates depending on the kind of material used and on the chemicophysical composition of the implant. Methods: The authors report the case of a 50-year-old man with a severe deformity of the forehead-supra orbital area as a result of a previous complex fronto-facial trauma treated in an emergency Unit. Secondary correction and reconstruction of the residual deformities were performed by using Cerament® Bone Void Filler, an alloplastic biphasic material, composed of 40% hydroxyapatite, 60% calcium sulfate and the radio-contrast agent iohexol. The unique ratio of hydroxyapatite and calcium sulfate is designed to enable Cerament to resorb at the same rate that bone forms. Calcium sulfate acts as a resorbable carrier for hydroxyapatite which is highly osteoconductive, promoting bone ingrowth.It seems to be a promising bone graft substitute in the management of bony irregularities in the fronto-orbital area. Conclusion: The patient was first hospitalized as the result of a serious craniofacial trauma. One year after the first emergency cranio-orbital reconstructive operation, a marked deformity of the frontal region appeared with a “grid effect” due to the inadequate plate-bony fixation of the fractures applied during the first bony recomposition and because it was not as rigid as it should have been . A secondary surgery for deformity correction was performed. The hardware was totally removed and the bony deformity smoothed, reshaped, covered and filled using Cerament® Bone Void Filler, a biomaterial. The patient recovered with a satisfactory cranium-forehead shape, no complications, and complete disappearance of a frowning look of the fronto-orbital region. Recently, increased use of bone substitutes in the reconstruction of bone defects has been fuelled by donor site complications associated with autologous bone harvesting. Cerament® BoneVoid Filler is a biphasic and injectable bone substitute that has a highly compressive strength and the ability to promote cancellous bone healing

Chapter 14—Calcium Sulfate-Based Bone Void Substitutes

2009 ◽  
pp. 260-260-11 ◽  
Author(s):  
CT Laurencin ◽  
M Agrawal ◽  
DS Katti ◽  
WO Haggard ◽  
KC Richelsoph ◽  
...  
Keyword(s):  
Calcium Sulfate ◽  
Bone Void

Calcium Sulfate Bone Void Filler: A Review and a Look Ahead

2000 ◽  
Vol 11 (4) ◽  
pp. 327-333 ◽  
Author(s):  
William S. Pietrzak ◽  
Robert Ronk
Keyword(s):  
Calcium Sulfate ◽  
Look Ahead ◽  
Bone Void Filler ◽  
Bone Void

The Treatment of Chronic Osteomyelitis with a Biodegradable Antibiotic-Impregnated Implant

2002 ◽  
Vol 10 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Steven Gitelis ◽  
Gregory T. Brebach
Keyword(s):  
Dead Space ◽  
Calcium Sulfate ◽  
Bone Repair ◽  
Chronic Osteomyelitis ◽  
Attractive Alternative ◽  
Local Antibiotics ◽  
Biodegradable Implant ◽  
Bone Void Filler ◽  
Custom Made ◽  
Bone Void

The use of local antibiotics from a biodegradable implant for chronic osteomyelitis is an attractive alternative. The implant delivers high tissue levels, obliterates dead space, aids bone repair and does not need to be removed. The purpose of this paper is to review our early clinical experience with custom-made calcium sulfate (Osteoset bone void filler) antibiotic-impregnated implants.

scholarly journals Evaluation of Antibiotic-Releasing Triphasic Bone Void Filler In-Vitro

2018 ◽  
Vol 9 (4) ◽  
pp. 55 ◽  
Author(s):  
Michael Harris ◽  
Hamza Ahmed ◽  
Leslie Pace ◽  
Jon Minter ◽  
Michael Neel ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Minimal Effect ◽  
Time Points ◽  
Dissolution Study ◽  
Infection Treatment ◽  
Bone Void Filler ◽  
Bone Void

Bone void fillers (BVFs) containing calcium sulfate, tricalcium phosphate (TCP), and hydroxyapatite can be loaded with antibiotics for infection treatment or prevention under surgeon-directed use. The aim of this study was to characterize the handling and elution properties of a triphasic BVF loaded with common antibiotics. BVF was mixed with vancomycin and/or tobramycin to form pellets, and the set time was recorded. A partial refreshment elution study was conducted with time points at 4, 8, and 24 h, as well as 2, 7, 14, 28, and 42 days. Effects on dissolution were evaluated in a 14-day dissolution study. Set time increased to over 1 h for groups containing tobramycin, although vancomycin had a minimal effect. Pellets continued to elute antibiotics throughout the 42-day elution study, suggesting efficacy for the treatment or prevention of orthopedic infections. BVF containing vancomycin or tobramycin showed similar dissolution at 14 days compared to BVF without antibiotics; however, BVF containing both antibiotics showed significantly more dissolution.

scholarly journals Complete Killing of Agar Lawn Biofilms by Systematic Spacing of Antibiotic-Loaded Calcium Sulfate Beads

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4052 ◽  
Author(s):  
Devendra H. Dusane ◽  
Jacob R. Brooks ◽  
Devin Sindeldecker ◽  
Casey W. Peters ◽  
Anthony Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Calcium Sulfate ◽  
Chronic Infections ◽  
Replica Plating ◽  
Joint Infections ◽  
Single Bead ◽  
Causative Agents ◽  
Biofilm Bacteria ◽  
Adequate Coverage ◽  
And Staphylococcus Aureus

Background: Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA) are the major causative agents of acute and chronic infections. Antibiotic-loaded calcium sulfate beads (ALCSB) are used in the management of musculoskeletal infections such as periprosthetic joint infections (PJI). Methods: To determine whether the number and spatial distribution of ALCSB are important factors to totally eradicate biofilms, ALCSBs containing vancomycin and tobramycin were placed on 24 h agar lawn biofilms as a single bead in the center, or as 16 beads placed as four clusters of four, a ring around the edge and as a group in the center or 19 beads evenly across the plate. Bioluminescence was used to assess spatial metabolic activity in real time. Replica plating was used to assess viability. Results: For both strains antibiotics released from the beads completely killed biofilm bacteria in a zone immediately adjacent to each bead. However, for PA extended incubation revealed the emergence of resistant colony phenotypes between the zone of eradication and the background lawn. The rate of biofilm clearing was greater when the beads were distributed evenly over the plate. Conclusions: Both number and distribution pattern of ALCSB are important to ensure adequate coverage of antibiotics required to eradicate biofilms.

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