Novel atlas of fiber directions built from ex-vivo diffusion tensor images of porcine hearts

2020 ◽  
Vol 187 ◽  
pp. 105200
Author(s):  
Mia Mojica ◽  
Mihaela Pop ◽  
Maxime Sermesant ◽  
Mehran Ebrahimi
Keyword(s):  
Diffusion Tensor ◽  
Ex Vivo ◽  
Diffusion Tensor Images

scholarly journals Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Antara Chatterjee ◽  
Rojan Saghian ◽  
Anna Dorogin ◽  
Lindsay S. Cahill ◽  
John G. Sled ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Diffusion Tensor ◽  
Ex Vivo ◽  
Muscle Cells ◽  
Pregnant Mouse ◽  
Collagen Fibers ◽  
Muscular Layer ◽  
Tissue Organization ◽  
Picrosirius Red

AbstractThe cervix is responsible for maintaining pregnancy, and its timely remodeling is essential for the proper delivery of a baby. Cervical insufficiency, or “weakness”, may lead to preterm birth, which causes infant morbidities and mortalities worldwide. We used a mouse model of pregnancy and term labor, to examine the cervical structure by histology (Masson Trichome and Picrosirius Red staining), immunohistochemistry (Hyaluronic Acid Binding Protein/HABP), and ex-vivo MRI (T2-weighted and diffusion tensor imaging), focusing on two regions of the cervix (i.e., endocervix and ectocervix). Our results show that mouse endocervix has a higher proportion of smooth muscle cells and collagen fibers per area, with more compact tissue structure, than the ectocervix. With advanced gestation, endocervical changes, indicative of impending delivery, are manifested in fewer smooth muscle cells, expansion of the extracellular space, and lower presence of collagen fibers. MRI detected three distinctive zones in pregnant mouse endocervix: (1) inner collagenous layer, (2) middle circular muscular layer, and (3) outer longitudinal muscular layer. Diffusion MRI images detected changes in tissue organization as gestation progressed suggesting the potential application of this technique to non-invasively monitor cervical changes that precede the onset of labor in women at risk for preterm delivery.

scholarly journals Direct segmentation of the major white matter tracts in diffusion tensor images

NeuroImage ◽  
2011 ◽  
Vol 58 (2) ◽  
pp. 458-468 ◽  
Author(s):  
Pierre-Louis Bazin ◽  
Chuyang Ye ◽  
John A. Bogovic ◽  
Navid Shiee ◽  
Daniel S. Reich ◽  
...  
Keyword(s):  
White Matter ◽  
Diffusion Tensor ◽  
White Matter Tracts ◽  
Diffusion Tensor Images

scholarly journals Bayesian regularization of diffusion tensor images

Biostatistics ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 784-799 ◽  
Author(s):  
J. Frandsen ◽  
A. Hobolth ◽  
L. Ostergaard ◽  
P. Vestergaard-Poulsen ◽  
E. B. Vedel Jensen
Keyword(s):  
Diffusion Tensor ◽  
Bayesian Regularization ◽  
Diffusion Tensor Images

Repeated intravenous infusion of mesenchymal stem cells for enhanced functional recovery in a rat model of chronic cerebral ischemia

2021 ◽  
pp. 1-10
Author(s):  
Mitsuhiro Takemura ◽  
Masanori Sasaki ◽  
Yuko Kataoka-Sasaki ◽  
Ryo Kiyose ◽  
Hiroshi Nagahama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Diffusion Tensor Imaging ◽  
Diffusion Tensor ◽  
Ex Vivo ◽  
Chronic Phase ◽  
Vehicle Group ◽  
Single Administration ◽  
Chronic Cerebral Ischemia ◽  
Neural Connections

OBJECTIVE Stroke is a major cause of long-term disability, and there are few effective treatments that improve function in patients during the chronic phase of stroke. Previous research has shown that single systemic infusion of mesenchymal stem cells (MSCs) improves motor function in acute and chronic cerebral ischemia models in rats. A possible mechanism that could explain such an event includes the enhanced neural connections between cerebral hemispheres that contribute to therapeutic effects. In the present study, repeated infusions (3 times at weekly intervals) of MSCs were administered in a rat model of chronic stroke to determine if multiple dosing facilitated plasticity in neural connections. METHODS The authors induced middle cerebral artery occlusion (MCAO) in rats and, 8 weeks thereafter, used them as a chronic stroke model. The rats with MCAO were randomized and intravenously infused with vehicle only (vehicle group); with MSCs at week 8 (single administration: MSC-1 group); or with MSCs at weeks 8, 9, and 10 (3 times, repeated administration: MSC-3 group) via femoral veins. Ischemic lesion volume and behavioral performance were examined. Fifteen weeks after induction of MCAO, the thickness of the corpus callosum (CC) was determined using Nissl staining. Immunohistochemical analysis of the CC was performed using anti-neurofilament antibody. Interhemispheric connections through the CC were assessed ex vivo by diffusion tensor imaging. RESULTS Motor recovery was better in the MSC-3 group than in the MSC-1 group. In each group, there was no change in the ischemic volume before and after infusion. However, both thickness and optical density of neurofilament staining in the CC were greater in the MSC-3 group, followed by the MSC-1 group, and then the vehicle group. The increased thickness and optical density of neurofilament in the CC correlated with motor function at 15 weeks following induction of MCAO. Preserved neural tracts that ran through interhemispheric connections via the CC were also more extensive in the MSC-3 group, followed by the MSC-1 group and then the vehicle group, as observed ex vivo using diffusion tensor imaging. CONCLUSIONS These results indicate that repeated systemic administration of MSCs over 3 weeks resulted in greater functional improvement as compared to single administration and/or vehicle infusion. In addition, administration of MSCs is associated with promotion of interhemispheric connectivity through the CC in the chronic phase of cerebral infarction.

scholarly journals Acute ex vivo changes in brain white matter diffusion tensor metrics

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0223211
Author(s):  
Matthew R. Walker ◽  
Jidan Zhong ◽  
Adam C. Waspe ◽  
Thomas Looi ◽  
Karolina Piorkowska ◽  
...  
Keyword(s):  
White Matter ◽  
Diffusion Tensor ◽  
Ex Vivo ◽  
Brain White Matter
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