Variants of CARD15/NOD2 Associated with Crohn's Disease and Blau Syndrome Differ from Wild Type NOD2 in Regulating Cytokine Secretion Induced by TLR2 and TLR4 Agonists in a Transduced Macrophage Cell Line

2007 ◽  
Vol 123 ◽  
pp. S135-S136
Author(s):  
Michael Davey ◽  
Zili Zhang ◽  
Tammy Martin ◽  
Holly Rosenzweig ◽  
Steven Planck ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Cell Line ◽  
Cytokine Secretion ◽  
Blau Syndrome ◽  
Wild Type ◽  
Macrophage Cell Line ◽  
Macrophage Cell

Low-level iron-dependent mutants ofListeria monocytogenesand their virulence in macrophages

2003 ◽  
Vol 49 (2) ◽  
pp. 78-84 ◽  
Author(s):  
Philippe Andre ◽  
Stéphanie Oberle ◽  
Véronique Specklin ◽  
Yves Lombard ◽  
Dominique Jean-Marie Vidon
Keyword(s):  
Listeria Monocytogenes ◽  
Cell Line ◽  
Parental Strain ◽  
Wild Strain ◽  
Wild Type ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Macrophage Cells ◽  
J774 Macrophage ◽  
Iron Requirement

Listeria monocytogenes is an opportunistic intracellular pathogen capable of growth within phagocytic cells that requires iron for growth and virulence expression. In the presence of an appropriate concentration of tropolone, an iron-chelating agent, growth of L. monocytogenes is completely inhibited. However, this inhibition can be relieved by addition of dopamine, norepinephrine, or ferric citrate. By selection on streptonigrin medium supplemented with tropolone and norepinephrine, we have obtained two spontaneous mutants, Lm-8 and Lm-15, with the same iron dependence but lower iron dependence than the wild-type Lm-B38. The association between iron requirement and virulence of the two mutants and the wild type was studied in the J774 macrophage cell line. One hour after phagocytosis by the J774 macrophage cell line, the two mutants and the parental strain displayed no difference in the number of phagocytosed bacteria. Twenty-four hours after phagocytosis, the number of bacteria within the surviving macrophages was identical for the wild strain and the two clones. However, only 40% of macrophage cells infected with Lm-8 and 90% of those infected with Lm-15 were alive after 24 h in comparison with macrophage cells infected with the parental strain Lm-B38. These data demonstrate that there is no direct correlation between iron requirement and virulence of L. monocytogenes in the J774 macrophage cell line.Key words: Listeria monocytogenes, iron, virulence, macrophages.

scholarly journals Proprotein Convertase 1/3 (PC1/3) in the Rat Alveolar Macrophage Cell Line NR8383: Localization, Trafficking and Effects on Cytokine Secretion

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61557 ◽  
Author(s):  
Hugo Gagnon ◽  
Sarah Refaie ◽  
Sandra Gagnon ◽  
Roxane Desjardins ◽  
Michel Salzet ◽  
...  
Keyword(s):  
Alveolar Macrophage ◽  
Cell Line ◽  
Cytokine Secretion ◽  
Macrophage Cell Line ◽  
Proprotein Convertase ◽  
Macrophage Cell

Immune Enhancing Effect of Medicinal Herb Extracts on a RAW 264.7 Macrophage Cell Line

2012 ◽  
Vol 41 (11) ◽  
pp. 1521-1527 ◽  
Author(s):  
A-Reum Yu ◽  
Ho-Young Park ◽  
In-Wook Choi ◽  
Yong-Kon Park ◽  
Hee-Do Hong ◽  
...  
Keyword(s):  
Cell Line ◽  
Medicinal Herb ◽  
Raw 264.7 ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Enhancing Effect ◽  
Raw 264.7 Macrophage ◽  
Herb Extracts
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