Su.10. Rapid Restoration of Anti-Islet Autoimmunity Following Transplantation of Islets with Native Insulin Sequence Versus Insulin Gene Knockout Islets with Mutated B16:a Insulin

2006 ◽  
Vol 119 ◽  
pp. S162-S163
Author(s):  
Maki Nakayama ◽  
Joshua Beilke ◽  
Jean Jasinski ◽  
Dongmei Miao ◽  
Masakazu Kobayashi ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Insulin Gene ◽  
Islet Autoimmunity ◽  
Sequence Versus ◽  
Rapid Restoration ◽  
Native Insulin

Immunization profiles and progression of islet autoimmunity in children at type 1 diabetes risk

2012 ◽  
Vol 7 (S 01) ◽  
Author(s):  
R Chmiel ◽  
S Krause ◽  
A Knopff ◽  
C Matzke ◽  
D Höfelmann ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Diabetes Risk ◽  
Islet Autoimmunity

Association between DNA-sequences flanking the insulin-gene and atherosclerosis

1985 ◽  
Vol 110 (4_Suppl) ◽  
pp. S35-S41 ◽  
Author(s):  
Jørn Nerup ◽  
Thomas Mandrup-Poulsen ◽  
David Owerbach ◽  
Klaus Johansen ◽  
Jette Ingerslev ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Insulin Gene

Production of prion gene knockout cow to prevent spontaneous bovine spongiform encephalopathy

2014 ◽  
Author(s):  
Noboru Manabe ◽  
Ichiro Onoyama ◽  
Junyou Li ◽  
Yutaka Sendai ◽  
Yoshito Aoyagi
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Gene Knockout ◽  
Spongiform Encephalopathy ◽  
Prion Gene

Analysis of ∼700 Human Tissue Samples Identifies microRNAs That Are Associated with, Predictive of, and Necessary for Insulin Gene Transcription

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2160-P
Author(s):  
ANAND HARDIKAR ◽  
WILSON WONG ◽  
MUGDHA JOGLEKAR ◽  
LOUISE T. DALGAARD ◽  
ALICIA JENKINS ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Human Tissue ◽  
Insulin Gene ◽  
Tissue Samples ◽  
Insulin Gene Transcription

107-OR: Novel Genetic Risk Factors Influence Islet Autoimmunity Progression

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 107-OR
Author(s):  
SUNA ONENGUT-GUMUSCU ◽  
UMA DEVI PAILA ◽  
WEI-MIN CHEN ◽  
AAKROSH RATAN ◽  
ZHENNAN ZHU ◽  
...  
Keyword(s):  
Risk Factors ◽  
Genetic Risk ◽  
Genetic Risk Factors ◽  
Islet Autoimmunity

Lack of association of the polymorphic locus in the 5'-flanking region of the human insulin gene and diabetes in American blacks

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 433-439 ◽  
Author(s):  
S. Elbein ◽  
P. Rotwein ◽  
M. A. Permutt ◽  
G. I. Bell ◽  
N. Sanz ◽  
...  
Keyword(s):  
Human Insulin ◽  
Polymorphic Locus ◽  
Insulin Gene ◽  
Human Insulin Gene ◽  
Flanking Region ◽  
American Blacks

Treatment with streptococcal preparation (OK-432) suppresses anti-islet autoimmunity and prevents diabetes in BB rats

Diabetes ◽  
1988 ◽  
Vol 37 (9) ◽  
pp. 1188-1194 ◽  
Author(s):  
J. Satoh ◽  
S. Shintani ◽  
K. Oya ◽  
S. Tanaka ◽  
T. Nobunaga ◽  
...  
Keyword(s):  
Islet Autoimmunity ◽  
Bb Rats ◽  
Streptococcal Preparation

Differentiating glucose toxicity from glucose desensitization: a new message from the insulin gene

Diabetes ◽  
1994 ◽  
Vol 43 (9) ◽  
pp. 1085-1089 ◽  
Author(s):  
R. P. Robertson ◽  
L. K. Olson ◽  
H. J. Zhang
Keyword(s):  
Insulin Gene ◽  
Glucose Toxicity

Cloning and Expression of Recombinant Human Insulin Gene in Pichia pastoris

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Pichia Pastoris ◽  
Human Insulin ◽  
Expression Vector ◽  
Expression System ◽  
Insulin Gene ◽  
Cloning And Expression ◽  
Pcr Analysis ◽  
Major Band ◽  
Human Insulin Gene ◽  
Pichia Pastoris Expression System

The main goal of the current study was cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand, by using two sets of specific primers contain EcoR1 and Notl restriction sites. The amplified preproinsulin gene fragment was double digested with EcoRI and Not 1 restriction enzymes, then inserted into pPIC9K expression vector. The new pPIC9K-hpi constructive expression vector was transformed by the heat-shock method into the E.coli DH5α competent cells. pPic9k –hpi, which was propagated in the positive transformant E. coli cells, was isolated from cells and then linearised by restriction enzyme SalI, then transformed into Pichia pastoris GS115 using electroporation method. Genomic DNA of His+ transformants cell was extracted and used as a template for PCR analysis. The results showed, that the pPic9k – hpi was successfully integrated into the P. pastoris genome, for selected His+ transformants clones on the anticipated band at 330 bp, which is corresponded to the theoretical molecular size of the human insulin gene. To follow the insulin expression in transformans, Tricine–SDS gel electrophoresis and Western blot analysis were conducted. The results showed a successful expression of recombinant protein was detected by the presence of a single major band with about (5.8 KDa) on the gel. These bands correspond well with the size of human insulin with the theoretical molecular weight (5.8 KDa).

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