OR.77. Decreased Il-13 Production by Lamina Propria Mononuclear Cells (LPMCS) Accompanies Ulcerative Colitis (UC) Remission and Clinical Response Induced By Interferon-β Treatment

2006 ◽  
Vol 119 ◽  
pp. S33-S34
Author(s):  
Peter Mannon ◽  
Ivan Fuss ◽  
Catherine Groden ◽  
Julia Friend ◽  
Zhiqiong Yang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Clinical Response ◽  
Lamina Propria ◽  
Mononuclear Cells ◽  
Interferon Β ◽  
Lamina Propria Mononuclear Cells

Increased production of soluble TLR2 by lamina propria mononuclear cells from ulcerative colitis patients

Immunobiology ◽  
2012 ◽  
Vol 217 (6) ◽  
pp. 634-642 ◽  
Author(s):  
Enzo Candia ◽  
David Díaz-Jiménez ◽  
Patricia Langjahr ◽  
Lucía E. Núñez ◽  
Marjorie de la Fuente ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Lamina Propria ◽  
Mononuclear Cells ◽  
Lamina Propria Mononuclear Cells

scholarly journals Lamina Propria Mononuclear Cells Express and Respond to Interleukin-2 Differently in Crohn's Disease and Ulcerative Colitis.

1996 ◽  
Vol 35 (9) ◽  
pp. 679-685 ◽  
Author(s):  
Masataka SHINODA ◽  
Jun-ichi HARUTA ◽  
Mitsune TANIMOTO ◽  
Takafumi ANDO ◽  
Takehiko HOSOKAWA ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Lamina Propria ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Lamina Propria Mononuclear Cells

Zinc Deficiency Activates the IL-23/Th17 Axis to Aggravate Experimental Colitis in Mice

2019 ◽  
Vol 14 (6) ◽  
pp. 856-866 ◽  
Author(s):  
Yasuki Higashimura ◽  
Tomohisa Takagi ◽  
Yuji Naito ◽  
Kazuhiko Uchiyama ◽  
Katsura Mizushima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lamina Propria ◽  
Zinc Deficiency ◽  
Mononuclear Cells ◽  
Interferon Regulatory Factor ◽  
Nuclear Accumulation ◽  
Regulatory Factor ◽  
Colonic Inflammation ◽  
Interferon Regulatory Factor 5 ◽  
Lamina Propria Mononuclear Cells

Abstract Background and Aims Patients with inflammatory bowel disease [IBD], especially Crohn’s disease, often develop zinc deficiency. However, the precise mechanisms by which zinc deficiency affects IBD pathology, particularly intestinal macrophage function, remain unclear. We studied the effects of zinc deficiency on the development and progression of colitis in mice. Methods To induce colitis, mice were treated with 2,4,6-trinitrobenzene sulphonic acid. Rag1−/− mice were then given injections of naïve CD4+CD62L+ T cells. The respective degrees of mucosal injury of mice that had received a zinc chelator (TPEN; N,N,N′,N′-tetrakis [2-pyridylmethyl]ethylenediamine) and of control mice were subsequently compared. Colonic lamina propria mononuclear cells were isolated by enzymatic digestion and were examined using flow cytometry. To generate mouse bone marrow-derived macrophages [BMDMs], bone marrow cells were stimulated with mouse macrophage-colony stimulating factor. Results Zinc deficiency aggravates colonic inflammation through the activation of type 17 helper T [Th17] cells in mice. Flow cytometric analysis revealed that zinc deficiency significantly increases the proportion of pro-inflammatory [M1] macrophages in colonic lamina propria mononuclear cells obtained from inflamed colon. Interferon-γ plus lipopolysaccharide-mediated M1 skewing alters the expression of zinc transporters in BMDMs and thereby decreases the intracellular free zinc. TPEN treatment mimicking the effects of the M1 skewing up-regulates IL-23p19 expression, which is strongly related to Th17 development. Furthermore, the nuclear accumulation of interferon-regulatory factor 5 is closely involved in IL-23p19 induction in zinc-deficient macrophages. Conclusions Zinc deficiency aggravates colonic inflammation through activation of the IL-23/Th17 axis. This activation is controlled by subcellular distribution of interferon-regulatory factor 5.

Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue

2007 ◽  
Vol 2 (10) ◽  
pp. 2307-2311 ◽  
Author(s):  
Benno Weigmann ◽  
Ingrid Tubbe ◽  
Daniel Seidel ◽  
Alex Nicolaev ◽  
Christoph Becker ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Mononuclear Cells ◽  
Colonic Tissue ◽  
Lamina Propria Mononuclear Cells
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