ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients

2006 ◽  
Vol 120 (3) ◽  
pp. 342-348 ◽  
Author(s):  
Monika Lindemann ◽  
Oliver Witzke ◽  
Peter Lütkes ◽  
Melanie Fiedler ◽  
Ernst Kreuzfelder ◽  
...  
Keyword(s):  
Influenza Vaccination ◽  
Kidney Transplant ◽  
Cellular Immunity ◽  
Elispot Assay ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Sensitive Tool

scholarly journals Establishment of an ELISpot Assay to Detect Cellular Immunity against S. pneumoniae in Vaccinated Kidney Transplant Recipients

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1438
Author(s):  
Anja Gäckler ◽  
Nils Mülling ◽  
Kim Völk ◽  
Benjamin Wilde ◽  
Ute Eisenberger ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Cellular Immunity ◽  
Elispot Assay ◽  
Pneumococcal Infection ◽  
Organ Transplant ◽  
Pneumococcal Vaccination ◽  
Cellular Responses ◽  
Interferon Γ ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients

In organ transplant recipients, the rate of invasive pneumococcal diseases is 25 times greater than in the general population. Vaccination against S. pneumoniae is recommended in this cohort because it reduces the incidence of this severe form of pneumococcal infection. Previous studies indicate that transplant recipients can produce specific antibodies after pneumococcal vaccination. However, it remains unclear if vaccination also induces specific cellular immunity. In the current study on 38 kidney transplant recipients, we established an interferon-γ ELISpot assay that can detect serotype-specific cellular responses against S. pneumoniae. The results indicate that sequential vaccination with the conjugated vaccine Prevenar 13 and the polysaccharide vaccine Pneumovax 23 led to an increase of serotype-specific cellular immunity. We observed the strongest responses against the serotypes 9N and 14, which are both components of Pneumovax 23. Cellular responses against S. pneumoniae correlated positively with specific IgG antibodies (r = 0.32, p = 0.12). In conclusion, this is the first report indicating that kidney transplant recipients can mount specific cellular responses after pneumococcal vaccination. The ELISpot we established will allow for further investigations. These could help to define, for example, factors influencing specific cellular immunity in immunocompromised cohorts or the duration of cellular immunity after vaccination.

scholarly journals Enzyme Linked Immunosorbent Spot (ELISPOT) Assay for Interferon-Gamma Independently Predicts Renal Function in Kidney Transplant Recipients

2003 ◽  
Vol 3 (7) ◽  
pp. 878-884 ◽  
Author(s):  
Donald E. Hricik ◽  
Victoria Rodriguez ◽  
Jocelyn Riley ◽  
Katherine Bryan ◽  
Magdalena Tary-Lehmann ◽  
...  
Keyword(s):  
Renal Function ◽  
Kidney Transplant ◽  
Interferon Gamma ◽  
Elispot Assay ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients

scholarly journals SP719IMPAIRED CELLULAR IMMUNITY PREDISPOSES KIDNEY TRANSPLANT RECIPIENTS TO THE DEVELOPMENT OF CANCER

2017 ◽  
Vol 32 (suppl_3) ◽  
pp. iii381-iii381
Author(s):  
Natalie Otto ◽  
Thomas Schachtner ◽  
Maik Stein ◽  
Petra Reinke
Keyword(s):  
Kidney Transplant ◽  
Cellular Immunity ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients
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