Patterns of Relative Telomere Length is Associated With hTERT Gene Expression in the Tissue of Patients With Breast Cancer

2019 ◽  
Vol 19 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Karuvaje Thriveni ◽  
Anisha Raju ◽  
Rekha V. Kumar ◽  
Swamyvelu Krishnamurthy ◽  
Ramesh Chaluvarayaswamy
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Telomere Length ◽  
Htert Gene ◽  
Relative Telomere Length

scholarly journals Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Aaron L. Slusher ◽  
Tiffany M. Zúñiga ◽  
Edmund O. Acevedo
Keyword(s):  
Gene Expression ◽  
Young Adults ◽  
Telomere Length ◽  
Immune Cell ◽  
Ex Vivo ◽  
Middle Aged ◽  
Human Telomerase Reverse Transcriptase ◽  
Negatively Associated ◽  
Htert Gene ◽  
Age Related

Age-related elevations in proinflammatory cytokines, known as inflamm-aging, are associated with shorter immune cell telomere lengths. Purpose. This study examined the relationship of plasma PTX3 concentrations, a biomarker of appropriate immune function, with telomere length in 15 middle-aged (40-64 years) and 15 young adults (20-31 years). In addition, PBMCs were isolated from middle-aged and young adults to examine their capacity to express a key mechanistic component of telomere length maintenance, human telomerase reverse transcriptase (hTERT), following ex vivo cellular stimulation. Methods. Plasma PTX3 and inflammatory cytokines (i.e., IL-6, IL-10, TGF-β, and TNF-α), PBMC telomere lengths, and PBMC hTERT gene expression and inflammatory protein secretion following exposure to LPS, PTX3, and PTX3+LPS were measured. Results. Aging was accompanied by the accumulation of centrally located visceral adipose tissue, without changes in body weight and BMI, and alterations in the systemic inflammatory milieu (decreased plasma PTX3 and TGF-β; increased TNF-α (p≤0.050)). In addition, shorter telomere lengths in middle-aged compared to young adults (p=0.011) were negatively associated with age, body fat percentages, and plasma TNF-α (r=−0.404, p=0.027; r=−0.427, p=0.019; and r=−0.323, p=0.041, respectively). Finally, the capacity of PBMCs to increase hTERT gene expression following ex vivo stimulation was impaired in middle-aged compared to young adults (p=0.033) and negatively associated with telomere lengths (r=0.353, p=0.028). Conclusions. Proinflammation and the impaired hTERT gene expression capacity of PBMCs may contribute to age-related telomere attrition and disease.

Relative telomere length and gene expression of shelterin complex proteins among vinyl chloride monomer‐exposed workers in China

2019 ◽  
Vol 60 (4) ◽  
pp. 361-367 ◽  
Author(s):  
Guo‐Qiao Zheng ◽  
Guang‐Hui Zhang ◽  
Han‐Tian Wu ◽  
Yu‐Ting Tu ◽  
Wei Tian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Telomere Length ◽  
Vinyl Chloride ◽  
Vinyl Chloride Monomer ◽  
Shelterin Complex ◽  
Relative Telomere Length ◽  
Exposed Workers

scholarly journals Nano-encapsulated metformin-curcumin in PLGA/PEG inhibits synergistically growth and hTERT gene expression in human breast cancer cells

2017 ◽  
Vol 46 (5) ◽  
pp. 917-925 ◽  
Author(s):  
Raana Farajzadeh ◽  
Younes Pilehvar-Soltanahmadi ◽  
Mehdi Dadashpour ◽  
Shahrzad Javidfar ◽  
Javid Lotfi-Attari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Htert Gene

scholarly journals Design and development of nanostructured co delivery of artemisinin and chrysin for targeting hTERT gene expression in breast cancer cell line: possible clinical application in cancer treatment

2021 ◽  
Author(s):  
Leila Khoshravan Azar ◽  
Mehdi Dadashpour ◽  
Akram Firouzi-Amandi ◽  
Nosratollah Zarghami
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Plga Nanoparticles ◽  
Preventive Strategy ◽  
Htert Gene

Abstract Background: Breast cancer is one of the most significant causes of female cancer death worldwide. To explore the possibility of a novel chemo-preventive strategy for improving breast cancer treatment, the anticancer effects of a combination two natural compounds, Artemisinin (Art) and Chrysin (Chr), against T47D breast cancer cells were investigated.Methods: For this purpose, Art and Chr were co-encapsulated in PEGylated PLGA nanoparticles (NPs) and evaluated for their therapeutic efficacy. The morphology and dynamic light scattering (DLS) analyses were carried out to optimize the Nano formulations. Drug release study was performed using the dialysis method and then the cytotoxic and inhibitory effect of individual and combined drugs on the expression level of hTERT in the T47D breast cell line was evaluated using MTT assay and qPCR, respectively. Results: The results showed that pure drugs and formulations exhibited dose-dependent cytotoxicity against T47D cells and especially, Art/Chr–PLGA/PEG NPs had a more synergistic anti-proliferative effect and significantly arrested the growth of cancer cells than the other groups. Real-time PCR results revealed that Art, Chr and combination of Art–Chr in pure and encapsulated forms inhibited hTERT gene expression. Conclusions: It was found that Art–Chr–PLGA/PEG NPs relative to pure combination could further decline hTERT expression in all concentrations. Our study demonstrated that Art–Chr–PLGA/PEG NPs based combinational therapy holds promising potential for the treatment of breast cancer.

scholarly journals A Prospective Study of Relative Telomere Length and Postmenopausal Breast Cancer Risk

2009 ◽  
Vol 18 (4) ◽  
pp. 1152-1156 ◽  
Author(s):  
Immaculata De Vivo ◽  
Jennifer Prescott ◽  
Jason Y.Y. Wong ◽  
Peter Kraft ◽  
Susan E. Hankinson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Prospective Study ◽  
Telomere Length ◽  
Postmenopausal Breast Cancer ◽  
Postmenopausal Breast Cancer Risk ◽  
Relative Telomere Length ◽  
A Prospective Study

scholarly journals Stabilization of Telomere Length and Karyotypic Stability Are Directly Correlated with the Level of hTERT Gene Expression in Primary Fibroblasts

2002 ◽  
Vol 277 (41) ◽  
pp. 38531-38539 ◽  
Author(s):  
Wei Cui ◽  
Samena Aslam ◽  
Judy Fletcher ◽  
Diana Wylie ◽  
Michael Clinton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Telomere Length ◽  
Htert Gene ◽  
Primary Fibroblasts

Amino-bisphosphonates decrease hTERT gene expression in breast cancer in vitro

2007 ◽  
Vol 19 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Luca Dalle Carbonare ◽  
Maria T. Valenti ◽  
Francesco Bertoldo ◽  
Arianna Fracalossi ◽  
Elena Balducci ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Htert Gene

scholarly journals Interleukin-6, -8, and TGF-β Secreted from Mesenchymal Stem Cells Show Functional Role in Reduction of Telomerase Activity of Leukemia Cell Via Wnt5a/β-Catenin and P53 Pathways

2020 ◽  
Vol 10 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Ezzatollah Fathi ◽  
Behnaz Valipour ◽  
Zohreh Sanaat ◽  
Hojjatollah Nozad Charoudeh ◽  
Raheleh Farahzadi
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Telomere Length ◽  
Real Time Pcr ◽  
Leukemia Cell ◽  
K562 Cells ◽  
Telomerase Activity ◽  
Htert Gene ◽  
Gene And Protein Expression

Purpose: The effect of mesenchymal stem cells (MSCs) on the immortality features of malignant cells, such as hematologic cancerous cells, are controversial, and the associated mechanisms are yet to be well understood. The aim of the present study was to investigate the in vitro effect of bone marrow-derived MSCs (BMSCs) on the chronic myeloid leukemia cell line K562 through telomere length measurements, telomerase activity assessments, and hTERT gene expression. The possible signaling pathways involved in this process, including Wnt-5a/β-catenin and P53, were also evaluated. Methods: Two cell populations (BMSCs and K562 cell line) were co-cultured on transwell plates for 7 days. Next, K562 cells were collected and subjected to quantitative real-time PCR, PCR-ELISA TRAP assay, and the ELISA sandwich technique for telomere length, hTERT gene expression, telomerase activity assay, and cytokine measurement, respectively. Also, the involvement of the mentioned signaling pathways in this process was reported by real-time PCR and Western blotting through gene and protein expression, respectively. Results: The results showed that BMSCs caused significant decreases in telomere length, telomerase activity, and the mRNA level of hTERT as a regulator of telomerase activity. The significant presence of interleukin (IL)-6, IL-8, and transforming growth factor beta (TGF-β) was obvious in the co-cultured media. Also, BMSCs significantly decreased and increased the gene and protein expression of β-catenin and P53, respectively. Conclusion: It was concluded that the mentioned effects of IL-6, IL-8, and TGF-β cytokines secreted from MSCs on K562 cells as therapeutic agents were applied by Wnt-5a/β-catenin and P53 pathways

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