USE OF CARDIAC MAGNETIC RESONANCE TO IDENTIFY MITRAL VALVE FIBROTIC CHANGES AFTER MYOCARDIAL INFARCTION: VALIDATION IN A LARGE ANIMAL MODEL

2017 ◽  
Vol 33 (10) ◽  
pp. S193
Author(s):  
M. Bouchard ◽  
O. Marsit ◽  
M. Clavel ◽  
M. Clisson ◽  
M. Drolet ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mitral Valve ◽  
Large Animal Model ◽  
Large Animal

scholarly journals TCT-825 In-Vivo Long Term Evaluation of a Novel Mitral Valve Regurgitation Therapy: Experience in a Preclinical Large Animal Model

2013 ◽  
Vol 62 (18) ◽  
pp. B249
Author(s):  
Athanasios Peppas ◽  
Jon Wilson ◽  
Yanping Cheng ◽  
Christopher Seguin ◽  
Masahiko Shibuya ◽  
...  
Keyword(s):  
Animal Model ◽  
Mitral Valve ◽  
Mitral Valve Regurgitation ◽  
Large Animal Model ◽  
Large Animal ◽  
Valve Regurgitation ◽  
Term Evaluation

scholarly journals A Nonthoracotomy Myocardial Infarction Model in an Ovine Using Autologous Platelets

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Tyler Spata ◽  
Daniel Bobek ◽  
Bryan A. Whitson ◽  
Sampath Parthasarathy ◽  
Peter J. Mohler ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Myocardial Infarct ◽  
Border Zone ◽  
Large Animal Model ◽  
Large Animal ◽  
Ovine Model ◽  
Intravascular Thrombosis ◽  
Serial Serum ◽  
Male Sheep

Objective. There is a paucity of a biological large animal model of myocardial infarction (MI). We hypothesized that, using autologous-aggregated platelets, we could create an ovine model that was reproducible and more closely mimicked the pathophysiology of MI.Methods. Mepacrine stained autologous platelets from male sheep (n=7) were used to create a myocardial infarction via catheter injection into the mid-left anterior descending (LAD) coronary artery. Serial daily serum troponin measurements were taken and tissue harvested on post-embolization day three. Immunofluorescence microscopy was used to detect the mepacrine-stained platelet-induced thrombus, and histology performed to identify three distinct myocardial (infarct, peri-ischemic “border zone,” and remote) zones.Results. Serial serum troponin levels (μg/mL) measured0.0±0.0at baseline and peaked at297.4±58.0on post-embolization day 1, followed by153.0±38.8on day 2 and76.7±19.8on day 3. Staining confirmed distinct myocardial regions of inflammation and fibrosis as well as mepacrine-stained platelets as the cause of intravascular thrombosis.Conclusion. We report a reproducible, unique model of a biological myocardial infarction in a large animal model. This technique can be used to study acute, regional myocardial changes following a thrombotic injury.

In vivo 19F MR inflammation imaging after myocardial infarction in a large animal model at 3 T

2018 ◽  
Vol 32 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Maik Rothe ◽  
Annika Jahn ◽  
Kilian Weiss ◽  
Jong-Hee Hwang ◽  
Julia Szendroedi ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Large Animal Model ◽  
Large Animal ◽  
Inflammation Imaging

Abstract 114: Allogeneic Cardiosphere-Derived Cells Are Safe and Effective in a Pig Model of Myocardial Infarction

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Rachel R Smith ◽  
Michelle Kreke ◽  
Konstantinos Malliaras ◽  
Hideaki Kanazawa ◽  
Christene A Huang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Safety Concern ◽  
Small Animal ◽  
Immunological Response ◽  
Balloon Occlusion ◽  
Large Animal Model ◽  
Large Animal ◽  
Translational Study ◽  
Post Infusion

Allogeneic cardiosphere-derived cells (CDCs) have proven safe and effective in a small animal model of myocardial infarction (MI), and have been shown to act primarily via paracrine mechanisms to stimulate endogenous regeneration. The present translational study tested allogeneic CDCs in a large animal model (mini-pigs). To establish a robust allogeneic model, all pigs were swine leukocyte antigen (SLA) typed by PCR. A male donor and female recipients with full SLA I, II mismatch were used. Pigs underwent balloon occlusion of the LAD for 2.5 hours, followed by reperfusion. Two weeks later, 12.5 million CDCs (n=8) or vehicle (n=6) were infused. Animals were sacrificed 2 weeks or 2 months post-infusion to assess the cellular (histology using a clinical rejection scale) and humoral (donor-specific antibodies, complement-dependent cytotoxicity) immune responses, as well as cardiac function (left ventriculography, hemodynamics, morphometry). Numerous in-life assessments for safety were performed and CDC engraftment was assessed by FISH for Y chromosome. All immunological assays indicated an undetectable response to CDCs. Cardiac enzymes and systemic inflammation showed no differences between groups. There were no systemic histological findings related to CDCs. CDCs did not permanently engraft, with <0.1% persisting 2 weeks post-infusion and none evident 2 months post. Despite evanescent engraftment, functional benefits were seen following infusion of CDCs. Ejection fraction (EF) and infarct size (IS) were significantly improved in CDC-treated animals compared to vehicle-treated 2 weeks post-infusion (EF: 48.6±2.1% vs 38.2±2.6%, p<0.05; IS: 9±2 vs 14±2%, p<0.05). At 2 months, EF still trended higher in CDC-treated animals (42±5 vs 32±10%, p=0.09), and IS still trended lower (14±4 vs 16±7%, p=0.60). Pressure-volume relationships revealed a trend for enhanced contractility in CDC-treated animals (Emax: 3.2±2.2 vs 1.0±1.1 mmHg/mL, p=0.26). The magnitude of the functional benefits was similar to that seen in a prior pig study using autologous CDCs. Overall, results of the present study demonstrate that allogeneic CDCs are largely equivalent to autologous in terms of efficacy, and elicit no detectable immunological response or safety concern.

Testing of Intra-Myocardial Cell Therapy in a Large Animal Model of Myocardial Infarction

2009 ◽  
Vol 18 ◽  
pp. S190
Author(s):  
Anthony J. White ◽  
Shuo-Tsan Lee ◽  
Satoshi Matsushita ◽  
Eduardo Marbán ◽  
Raj Makkar
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Cell Therapy ◽  
Myocardial Cell ◽  
Large Animal Model ◽  
Large Animal

Canine model of convection-enhanced delivery of liposomes containing CPT-11 monitored with real-time magnetic resonance imaging

2008 ◽  
Vol 108 (5) ◽  
pp. 989-998 ◽  
Author(s):  
Peter J. Dickinson ◽  
Richard A. Lecouteur ◽  
Robert J. Higgins ◽  
John R. Bringas ◽  
Byron Roberts ◽  
...  
Keyword(s):  
Animal Model ◽  
Magnetic Resonance ◽  
Mr Imaging ◽  
Real Time ◽  
Canine Model ◽  
Large Animal Model ◽  
Large Animal ◽  
Convection Enhanced Delivery ◽  
Liposomal Nanoparticles ◽  
Volumes Of Distribution

Object Many factors relating to the safety and efficacy of convection-enhanced delivery (CED) into intracranial tumors are poorly understood. To investigate these factors further and establish a more clinically relevant large animal model, with the potential to investigate CED in large, spontaneous tumors, the authors developed a magnetic resonance (MR) imaging–compatible system for CED of liposomal nanoparticles into the canine brain, incorporating real-time MR imaging. Additionally any possible toxicity of liposomes containing Gd and the chemotherapeutic agent irinotecan (CPT-11) was assessed following direct intraparenchymal delivery. Methods Four healthy laboratory dogs were infused with liposomes containing Gd, rhodamine, or CPT-11. Convection-enhanced delivery was monitored in real time by sequential MR imaging, and the volumes of distribution were calculated from MR images and histological sections. Assessment of any toxicity was based on clinical and histopathological evaluation. Convection-enhanced delivery resulted in robust volumes of distribution in both gray and white matter, and real-time MR imaging allowed accurate calculation of volumes and pathways of distribution. Results Infusion variability was greatest in the gray matter, and was associated with leakage into ventricular or subarachnoid spaces. Complications were minimal and included mild transient proprioceptive deficits, focal hemorrhage in 1 dog, and focal, mild perivascular, nonsuppurative encephalitis in 1 dog. Conclusions Convection-enhanced delivery of liposomal Gd/CPT-11 is associated with minimal adverse effects in a large animal model, and further assessment for use in clinical patients is warranted. Future studies investigating real-time monitored CED in spontaneous gliomas in canines are feasible and will provide a unique, clinically relevant large animal translational model for testing this and other therapeutic strategies.

CHALLENGING VOLTAGE MAPPING FOR SCAR DECHANNELING IN A LARGE ANIMAL MODEL OF MYOCARDIAL INFARCTION

2018 ◽  
Vol 71 (11) ◽  
pp. A304
Author(s):  
Tobias Plenge ◽  
Johannes Terporten ◽  
Alexandra Maul ◽  
Arian Sultan ◽  
Jakob Lueker ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Animal Model ◽  
Large Animal Model ◽  
Large Animal ◽  
Voltage Mapping
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