GLYOXYLASE 1 OVER EXPRESSION ATTENUATES THE ADVERSE EFFECTS OF CHRONIC HYPERGLYCEMIA ON THE THERAPEUTIC REGENERATION BY EXPLANT DERIVED CARDIAC STEM CELLS

2016 ◽  
Vol 32 (10) ◽  
pp. S292
Author(s):  
M. Villanueva ◽  
D.R. Davis
Keyword(s):  
Stem Cells ◽  
Adverse Effects ◽  
Cardiac Stem Cells ◽  
Over Expression ◽  
Chronic Hyperglycemia

OVER-EXPRESSION OF SDF1α ENHANCES CARDIAC REPAIR BY CARDIAC STEM CELLS

2014 ◽  
Vol 30 (10) ◽  
pp. S185
Author(s):  
E.L. Tilokee ◽  
R. Jackson ◽  
A. Mayfield ◽  
N. Latham ◽  
B. Ye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Repair ◽  
Cardiac Stem Cells ◽  
Over Expression

Abstract 15747: Over-Expression of Insulin-Like Growth Factor-1 by Human Cardiac Stem Cells Enhances Long-Term Retention of Transplanted Cells While Promoting Salvage of Injured Myocardium

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Robyn Jackson ◽  
Everad L Tilokee ◽  
Nicholas Latham ◽  
Bin Ye ◽  
Munir Boodhwani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Annexin V ◽  
Cardiac Repair ◽  
Population Doubling ◽  
Cardiac Stem Cells ◽  
Insulin Like Growth Factor ◽  
Over Expression ◽  
Myocardial Apoptosis

Background: Insulin-like growth factor (IGF-1) is a potent pro-survival cytokine that is not robustly expressed by human cardiac stem cells (CSCs). Previously, we have shown that paracrine engineering of CSCs with IGF-1 improves cell-mediated cardiac repair. Here, we explore the mechanisms underlying IGF-1 enhanced cardiac repair by CSCs. Methods/Results: Sub-culture of isolated c-Kit+, CD90+ and lineage negative cells (c-Kit-/CD90-) demonstrated that the natural low level production of IGF-1 by CSCs (149±16 pg/ml*mg) is secreted by all 3 sub-populations. After culture in hypoxic reduced serum media, lentiviral mediated over-expression of IGF-1 enhanced proliferation (population doubling time: 1.4±1.7 vs.-0.9±1.2 and -1.9±2.4 days, respectively; p≤0.01), expression of pro-survival transcripts (AKT, ERK and MAPK pathways) and pro-survival proteins (Bcl-2, Bcl-x, HIF-1a; p≤0.01) while decreasing expression of apoptotic markers (3.5±0.9 and 3.7±0.9 fold less annexin V; p≤0.01) as compared to GFP- and non-transduced CSCs. The high expression of the IGF-1 (79±3%) or the insulin receptor (61±5%) on CSCs suggests that autocrine pro-survival pre-conditioning underlies these effects. Direct and indirect co-culture of CSCs with neonatal rat ventricular cardiomyocytes (NRVMs) within hypoxic conditions demonstrated that IGF-1 promoted indirect myocardial repair by increasing NRVM viability and pro-survival signaling (Bcl2+; p≤0.01) while reducing apoptosis (annexin V+; p≤0.05) as compared GFP- or non-transduced CSCs. Transplant of CSCs genetically engineered to over-express IGF-1 into immunodeficient mice one week after infarction boosted IGF-1 content within infarcted tissue by 2.9±0.2 fold (p=0.004) and long-term engraftment (+4 weeks human alu content increased by 9.1±4 fold; p=0.05) while reducing myocardial apoptosis (3.4±0.3 and 2.5±0.5 fold reduction expression of Bax and p53, respectively; p<0.05) and long-term myocardial scarring (+ 4 weeks 2.2±0.4 fold less; p=0.01) as compared to GFP-transduced CSCs. Conclusions: Transplantation of IGF-1 enriched CSCs enhances cardiac repair by boosting transplant cell survival and reducing myocardial apoptosis to improve myocardial function and salvage of damaged myocardium.

Influence of MWNTs/PMMA Thin Film on Cellular Behavior of Cardiac Stem Cells From Brown Adipose

2013 ◽  
Vol 40 (10) ◽  
pp. 1070
Author(s):  
Cui-Mi DUAN ◽  
Hong-Yu SUN ◽  
Ye YUAN ◽  
Zhi-Qiang LIU ◽  
Rong-Yu TANG ◽  
...  
Keyword(s):  
Thin Film ◽  
Stem Cells ◽  
Cardiac Stem Cells ◽  
Cellular Behavior ◽  
Brown Adipose

High Glucose Affects the Cytotoxic Potential of Rapamycin, Metformin and Hydrogen Peroxide in Cultured Human Mesenchymal Stem Cells

2019 ◽  
Vol 19 (9) ◽  
pp. 688-698 ◽  
Author(s):  
Azam Roohi ◽  
Mahin Nikougoftar ◽  
Hamed Montazeri ◽  
Shadisadat Navabi ◽  
Fazel Shokri ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
High Glucose ◽  
Cell Cycle Distribution ◽  
Efficient Treatment ◽  
Chronic Hyperglycemia ◽  
Placental Mesenchymal Stem Cells

Background: Oxidative stress and chronic hyperglycemia are two major side effects of type 2 diabetes affecting all cell types including mesenchymal stem cells (MSCs). As a cell therapy choice, understanding the behavior of MSCs will provide crucial information for efficient treatment. Methods: Placental mesenchymal stem cells were treated with various concentrations of glucose, metformin, rapamycin, and hydrogen peroxide to monitor their viability and cell cycle distribution. Cellular viability was examined via the MTT assay. Cell cycle distribution was studied by propidium iodide staining and apoptosis was determined using Annexin Vpropidium iodide staining and flow cytometry. Involvement of potential signaling pathways was evaluated by Western blotting for activation of Akt, P70S6K, and AMPK. Results: The results indicated that high glucose augmented cell viability and reduced metformin toxic potential. However, the hydrogen peroxide and rapamycin toxicities were exacerbated. Conclusion: Our findings suggest that high glucose concentration has a major effect on placental mesenchymal stem cell viability in the presence of rapamycin, metformin and hydrogen peroxide in culture.

High-Speed Tracking of Murine Cardiac Stem Cells by Harmonic Nanodoublers

Small ◽  
2012 ◽  
Vol 8 (17) ◽  
pp. 2752-2756 ◽  
Author(s):  
Thibaud Magouroux ◽  
Jerome Extermann ◽  
Pernilla Hoffmann ◽  
Yannick Mugnier ◽  
Ronan Le Dantec ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Speed ◽  
Cardiac Stem Cells ◽  
Speed Tracking
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