Biocompatibility of Novel Ferromagnetic, Nanoprobes Coupled to Bone Marrow Derived Multipotential Mesenchymal Stromal/Stem Cells for Cell Tracking in Cardiac Cell Therapy

2013 ◽  
Vol 29 (10) ◽  
pp. S346-S347
Author(s):  
C. Sid-Otmane ◽  
W. Zhou ◽  
X.F. Zhang ◽  
T. Veres ◽  
H.Q. Ly
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Therapy ◽  
Cell Tracking ◽  
Cardiac Cell ◽  
Cardiac Cell Therapy ◽  
Stromal Stem Cells

Abstract 3420: Potential of Human Mesenchymal Stem Cells to Differentiate into Cardiomyocytes Depends on Donor Age and Donor Tissue

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
John Van Tuyn ◽  
Daniel A Pijnappels ◽  
Marlieke L Haeck ◽  
Shoshan Knaän-Shanzer ◽  
Sicco Scherjon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Cord Blood ◽  
Umbilical Cord Blood ◽  
Human Mesenchymal Stem Cells ◽  
Cardiac Cell ◽  
Donor Age ◽  
Cardiac Cell Therapy

Treatment of damaged myocardium using autologous adult stem cells is under intense investigation as this may regenerate lost myocardium, thereby improving cardiac function. Initial cardiac cell therapy trials using various adult stem cell types have shown beneficial effects. However in contrast to earlier animal studies no true, quantitative reconstitution of myocardium has been observed. We hypothesized that the cardiomyogenic potential of stem cells depends on donor age and cell source. We used an in vitro differentiation protocol that employs co-culture of enhanced green fluorescent protein-labeled human mesenchymal stem cells (hMSCs) with neonatal rat cardiomyocytes (nrCMCs) for 13 days, without additional chemical or mutagenic stimulation. hMSCs were either derived from adult patients, or from human fetal tissue (gestational age 18 –22 weeks) or post natally collected umbilical cord blood. Differentiation of hMSCs was assessed by the presence of a properly developed sarcomere using immunological labeling for cardiac troponin I, myosin heavy chain and actinin. Functionality of newly differentiated cardiomyocytes was assessed by the presence of a functional calcium transient in conjunction with myocyte contraction. Mesenchymal stem cells isolated from clinically relevant sources (bone marrow, fat, and synovium) of adult patients are unable to adopt a cardiomyocyte phenotype upon co-incubation with nrCMCs. In contrast, MSCs from fetal donor material all give rise to functional cardiomyocytes, although with varying efficiency (umbilical cord blood: 6.8%±3.0, bone marrow: 0.4%±1.4, liver: 1.8%±0.8, lung: 1.4%±1.3, amnion: 9.3%±5.0 [mean±SD, n=3]). Using short hairpin RNA-mediated knockdown of connexin 43 (Cx43) gene expression, we found that sarcomeric development (organization) depends on the presence of Cx43 in the differentiating stem cells. Both donor age and the tissue source of human MSCs critically influence their cardiomyogenic differentiation potential. These results suggest that these non-embryonic, fetal cells may potentially be clinically useful for cardiac cell therapy.

Placenta-derived mesenchymal stem cells for allogenic cardiac cell therapy

2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
R Roy ◽  
M Kukucka ◽  
D Messroghli ◽  
A Brodarac ◽  
M Becher ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Cardiac Cell ◽  
Cardiac Cell Therapy

scholarly journals The Presence of Apoptotic Bone Marrow Cells Impairs the Efficacy of Cardiac Cell Therapy

2011 ◽  
Vol 20 (7) ◽  
pp. 1087-1097 ◽  
Author(s):  
Frederic Mouquet ◽  
Gilles Lemesle ◽  
Cedric Delhaye ◽  
Clement Charbonnel ◽  
Alexandre Ung ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Therapy ◽  
Bone Marrow Cells ◽  
Cardiac Cell ◽  
Cardiac Cell Therapy ◽  
Marrow Cells

Predifferentiated Adult Stem Cells and Matrices for Cardiac Cell Therapy

2010 ◽  
Vol 18 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Cristiano Spadaccio ◽  
Emmanuel Chachques ◽  
Massimo Chello ◽  
Elvio Covino ◽  
Juan C Chachques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Therapy ◽  
Adult Stem Cells ◽  
Cardiac Cell ◽  
Cardiac Cell Therapy
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