379 Human Leukocyte Antigen-G Inhibits Human Coronary Artery Smooth Muscle Cell Proliferation

2012 ◽  
Vol 28 (5) ◽  
pp. S240-S241
Author(s):  
A.G. Mociornita ◽  
L. Tumiati ◽  
L. Grosman-Rimon ◽  
H.J. Ross ◽  
R. Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Human Leukocyte Antigen ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Human Leukocyte ◽  
Human Coronary Artery ◽  
Leukocyte Antigen ◽  
Human Leukocyte Antigen G

scholarly journals Adenosine Attenuates Human Coronary Artery Smooth Muscle Cell Proliferation by Inhibiting Multiple Signaling Pathways That Converge on Cyclin D

Hypertension ◽  
2015 ◽  
Vol 66 (6) ◽  
pp. 1207-1219 ◽  
Author(s):  
Raghvendra K. Dubey ◽  
Jürgen Fingerle ◽  
Delbert G. Gillespie ◽  
Zaichuan Mi ◽  
Marinella Rosselli ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Smooth Muscle Cell ◽  
Signaling Pathways ◽  
Muscle Cell ◽  
Smooth Muscle Cell Proliferation ◽  
Cyclin D ◽  
Human Coronary Artery ◽  
Multiple Signaling

scholarly journals Bindarit Inhibits Human Coronary Artery Smooth Muscle Cell Proliferation, Migration and Phenotypic Switching

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47464 ◽  
Author(s):  
Marcella Maddaluno ◽  
Gianluca Grassia ◽  
Maria Vittoria Di Lauro ◽  
Antonio Parisi ◽  
Francesco Maione ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Coronary Artery ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Smooth Muscle Cell Proliferation ◽  
Phenotypic Switching ◽  
Human Coronary Artery

Abstract 451: Oxygen Regulation of Human Coronary Artery Smooth Muscle Cell Proliferation and Migration

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Mingming Yang ◽  
Tomoko Kamishima ◽  
Caroline Dart ◽  
John M Quayle
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Coronary Artery ◽  
Cell Viability ◽  
Smooth Muscle Cell ◽  
Human Coronary Artery ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

Introduction: Intimal thickening of blood vessels, a hallmark of several vascular diseases including atherosclerosis and a potential point of therapeutic intervention, is caused by vascular smooth muscle cell proliferation and migration. It has been suggested that oxygen availability in vessels not only regulates behavior of smooth muscle cells but also serves as a trigger that may lead to pathological responses. In this study we determined whether hypoxia elicits proliferative and migratory responses in Human Coronary Artery SMCs (HCASMCs). Methods: Proliferation of HCASMCs was assessed using a 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SMCs were plated in 96-well plates (n=5), serum starved, and then placed under hypoxic or normoxic conditions for 2, 4 and 6 days (2D/4D/6D) before MTT was added to each well. Absorbance at the wavelength 570 nm was read on an ELISA plate reader, and percent change in cell viability was determined and normalized to control (cell viability under normoxia). Cell migration was characterized by scratch-wound assay. SMCs were seeded in 6 well plates overnight (n=3), then a ‘scratch’ on the cell monolayer was created for each well before putting into different oxygen levels for 4 hours, 12 hours and 24 hours. Images were captured at the beginning and at intervals during cell migration to close the scratch, and the degree of migration was determined by comparing the images. Results: Compared to normoxic condition, cell number changed to 118.1%±1.3% in 5% O 2 (p<0.05) and 98.2%%±1.9% in 1% O 2 after 2D; to 151.9% ±8.5% in 5% O 2 (p<0.001) and 119.4%±5.0% in 1% O 2 (p<0.05) after 4D; and to 163.0%±4.3% in 5% O 2 (p<0.001) and 120.3%±2.2% in 1% O 2 (p<0.05) after 6D. In the cell migration assay, the difference in migration rate between different groups after 4 hours was not obvious, but there was a significant difference after 12 hours (29.3%±1.3% closure in normoxia vs 39.8%±1.9% in 5% O 2 vs 40.9%±3.5% in 1% O 2 , p<0.05) and 24 hours (71.5%±4.4% in normoxia vs 87.2%±2.2% in 5% O 2 vs 87.5%±3.1% in 1% O 2 , p<0.05). Conclusion: Our studies reveal that hypoxia induces both proliferation and migration of HCASMCs.

Effect of the Endothelin Family of Peptides on Human Coronary Artery Smooth-Muscle Cell Migration

1998 ◽  
Vol 31 ◽  
pp. S84-S89 ◽  
Author(s):  
Masakazu Kohno ◽  
Koji Yokokawa ◽  
Kenichi Yasunari ◽  
Hiroaki Kano ◽  
Mieko Minami ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Migration ◽  
Coronary Artery ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Human Coronary Artery ◽  
Smooth Muscle Cell Migration
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