scholarly journals Identification of powdery mildew resistance loci in wheat by integrating genome-wide association study (GWAS) and linkage mapping

2019 ◽  
Vol 7 (3) ◽  
pp. 294-306 ◽  
Author(s):  
Genqiao Li ◽  
Xiangyang Xu ◽  
Chengcheng Tan ◽  
Brett F. Carver ◽  
Guihua Bai ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Association Study ◽  
Linkage Mapping ◽  
Powdery Mildew Resistance ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Mildew Resistance ◽  
Genome Wide

scholarly journals Linkage Mapping and Genome-Wide Association Study Reveals Conservative QTLs and Candidate Genes for Fusarium Rot Resistance in Maize

2020 ◽  
Author(s):  
Yabin Wu ◽  
Zijian Zhou ◽  
Chaopei Dong ◽  
Jiafa Chen ◽  
Junqiang Ding ◽  
...  
Keyword(s):  
Association Study ◽  
Candidate Genes ◽  
Phenotypic Variation ◽  
Linkage Mapping ◽  
Genome Wide Association Study ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Genome Wide Association ◽  
Ear Rot ◽  
Genome Wide

Abstract Background: Fusarium ear rot (FER) caused by Fusarium verticillioides is a major disease of maize that reduces grain yield and quality globally. However, there have been few reports of major loci for FER were verified and cloned. Result: To gain a comprehensive understanding of the genetic basis of natural variation in FER resistance, a recombinant inbred lines (RIL) population and one panel of inbred lines were used to map quantitative trait loci (QTL) for resistance. As a result, a total of 10 QTL were identified by linkage mapping under four environments, which were located on six chromosomes and explained 1.0%-7.1% of the phenotypic variation. Epistatic mapping detected four pairs of QTL that showed significant epistasis effects, explaining 2.1%-3.0% of the phenotypic variation. Additionally, 18 single nucleotide polymorphisms (SNPs) were identified across the whole genome by genome-wide association study (GWAS) under five environments. Compared linkage and association mapping revealed five common intervals located on chromosomes 3, 4, and 5 associated with FER resistance, four of which were verified in different near-isogenic lines (NILs) populations. GWAS identified three candidate genes in these consistent intervals, which belonged to the Glutaredoxin protein family , actin-depolymerizing factors (ADFs), and AMP-binding proteins. In addition, two verified FER QTL regions were found consistent with Fusarium cob rot (FCR) and Fusarium seed rot (FSR). Conclusions: These results revealed that multi pathways were involved in FER resistance, which was a complex trait that was controlled by multiple genes with minor effects, and provided important QTL and genes, which could be used in molecular breeding for resistance.

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