scholarly journals Separation of transition and heavy metals using stationary phase gradients and thin layer chromatography

2016 ◽  
Vol 1446 ◽  
pp. 141-148 ◽  
Author(s):  
Stacy L. Stegall ◽  
Kayesh M. Ashraf ◽  
Julie R. Moye ◽  
Daniel A. Higgins ◽  
Maryanne M. Collinson
Keyword(s):  
Heavy Metals ◽  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Transition And Heavy Metals ◽  
Layer Chromatography

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

scholarly journals A New Zinc-based Metal Organic Framework as a Stationary Phase for Thin Layer Chromatography

2020 ◽  
pp. 107-117
Author(s):  
Mouhammad Abu Rasheed ◽  
Ahmad Alshaghel ◽  
Amir Alhaj Sakur
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Surface Area ◽  
High Surface ◽  
Ultra Sound ◽  
Diffuse Reflection Spectroscopy ◽  
Scanning Calorimetry ◽  
Metal Organic ◽  
Layer Chromatography

Metal organic frameworks (MOFs) are a variety of micro-porous materials which have high surface area, and permanent porosity making them possible options as chromatographic stationary phases. Herein we reported the synthesis and characterization of a new MOF structure and its utilization as a stationary phase for thin layer chromatography (TLC). [Zn(BMAB).DMF]n is a zinc-based MOF with an organic linker consists of chemically distinct binding groups which is 4-{[(1h-1,2,3-benzotriazol-1-yl)methyl]amino}benzoic acid (BMAB). This MOF was synthesized using ultra sound assisted reaction process, then activated via solvent exchange protocol to preserve its porous structure. FT-IR, UV-diffuse reflection spectroscopy (UV-DRS) and differential scanning calorimetry (DSC) were performed to characterize the synthesized MOF. Integrated data from "loss on desolvation" and atomic absorption spectrophotometry (AAS) measurements were used to define the chemical composition of the synthesized material. A specific surface area of 122.9 m2/g was determined for the activated MOF using methylene blue langmuir isotherm method. TLC plates were prepared from the activated form of the structure to investigate its chromatographic characteristics by utilizing it to separate a model mixture of benzidine and o-tolidine using n-propanol: Chloroform: Acetonitrile (50:30:20, v/v/v) as a mobile phase. The retardation factors (Rf), separation factor, and resolution (Rs) were determined via densitometric method at 310 nm to be 0.45 and 0.63 (α=2.08, Rs=1.61) for o-tolidine and benzidine; respectively. The plate was then visualized using iodine chamber method to confirm a successful separation.

scholarly journals PRODUCTION AND PARTIAL CHARACTERIZATION OF PIGMENTS PRODUCED BY KOCURIA SP BRI 36: INFLUENCE OF HEAVY METALS

2017 ◽  
Vol 9 (10) ◽  
pp. 137 ◽  
Author(s):  
Anuradha Mulik ◽  
Priyanka Kumbhar ◽  
Rama Bhadekar
Keyword(s):  
Heavy Metals ◽  
Antimicrobial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pigment Production ◽  
Orange Pigment ◽  
Maximum Stability ◽  
Tlc Analysis ◽  
Physical And Chemical ◽  
Layer Chromatography

Objective: To study the production of pigments by Kocuria sp. BRI 36, their characteristics and influence of heavy metals on pigments.Methods: The effects of various physical and chemical parameters on pigments production by Kocuria sp. BRI 36 were examined. Pigments were extracted and partially characterised by Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR). The effects of heavy metals such as Pb2+, Cd 2+, Ni2+ and Cr3+ were studied on pigment production. Antimicrobial activity and stability studies of crude pigment were also conducted.Results: Kocuria sp. BRI 36 isolated from cold oceanic region maximally produced red-orange pigment in presence of glucose (5% w/v) and protease peptone (0.2% w/v) at pH 7.5, 10±1 °C. Thin layer chromatography (TLC) analysis revealed the occurrence of three different compounds in the crude pigment belonging to carotenoid and xanthophyll group. Metals like Ni2+ and Cr3+ adversely affected pigment production while Pb2+and Cd2+enhanced the yield. The significant features of Kocuria sp. BRI 36 pigment are i) antimicrobial activity against Gram-positive and Gram-negative bacteria, ii) maximum stability at pH 7.5 and 10±1 °C and iii) ~38% color loss at 50±1 °C in 5 h.Conclusion: Our results suggest application potential of Kocuria sp. BRI 36 pigments in various biotechnological fields.

scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

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