Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection

2014 ◽  
Vol 1337 ◽  
pp. 67-74 ◽  
Author(s):  
Luís Raiado-Pereira ◽  
Jonathan De La Vega ◽  
D. Miguel F. Prazeres ◽  
Marília Mateus
Keyword(s):  
Mammalian Cells ◽  
Deoxyribonucleic Acid ◽  
Membrane Chromatography

Recovery from Sub-lethal X-ray Damage of Mammalian Cells during Inhibition of Synthesis of Deoxyribonucleic Acid

Nature ◽  
1964 ◽  
Vol 204 (4958) ◽  
pp. 598-599 ◽  
Author(s):  
J. H. KIM ◽  
M. L. EIDINOFF ◽  
J. S. LAUGHLIN
Keyword(s):  
Mammalian Cells ◽  
Deoxyribonucleic Acid ◽  
X Ray

USE OF I125-LABELED 5-IODO-2′-DEOXYURIDINE FOR THE MEASUREMENT OF DNA SYNTHESIS IN MAMMALIAN CELLS IN VITRO

1963 ◽  
Vol 41 (11) ◽  
pp. 2343-2351 ◽  
Author(s):  
S. Mak ◽  
J. E. Till
Keyword(s):  
Dna Synthesis ◽  
Mammalian Cells ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
X Rays ◽  
Beta Particles ◽  
Deoxyribonucleic Acid Synthesis ◽  
Gamma Emitter

The use of isotopically labeled 5-iodo-2′-deoxyuridine (I125UdR) for determination of the rate of deoxyribonucleic acid synthesis in mammalian cells in vitro has been investigated. The results obtained indicate that for this purpose I125UdR is a suitable substitute for the more commonly used DNA precursor, tritium-labeled thymidine (H3TdR). I125UdR appears to be incorporated specifically into the DNA of cells in culture, and has been demonstrated to compete with H3TdR, although the Km for H3TdR was smaller than that of I125UdR by a factor of approximately 4. The amount of label incorporated into DNA of cells increased linearly with time. When the rate of DNA synthesis was reduced by exposure of the cells to various doses of X-rays, the ratio of I125UdR incorporation to H3TdR incorporation into DNA of cells was found to be a constant, which supports the view that uptake of the analogue provides as reliable an indication of effects upon the rate of DNA synthesis as does that of H3TdR. The chief advantage of I125UdR over H3TdR is that I125 is a gamma emitter, so that the difficulties encountered in detection of the low energy beta particles from H3 may be avoided.

Studies on the biosynthesis of deoxyribonucleic acid by extracts of mammalian cells III. Net synthesis of polynucleotides

1960 ◽  
Vol 37 (2) ◽  
pp. 243-250 ◽  
Author(s):  
R.M.S. Smellie ◽  
E.D. Gray ◽  
H.M. Keir ◽  
J. Richards ◽  
D. Bell ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Deoxyribonucleic Acid
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