Separation of polyethylene glycols and their amino-substituted derivatives by high-performance gel filtration chromatography at low ionic strength with refractive index detection

2004 ◽  
Vol 1046 (1-2) ◽  
pp. 121-126 ◽  
Author(s):  
Min Liu ◽  
Cao Xie ◽  
Wen Xu ◽  
Weiyue Lu
Keyword(s):  
Refractive Index ◽  
Ionic Strength ◽  
High Performance ◽  
Gel Filtration ◽  
Polyethylene Glycols ◽  
Gel Filtration Chromatography ◽  
Low Ionic Strength ◽  
Refractive Index Detection

scholarly journals Analysis of serum lipoproteins by high performance gel filtration chromatography.

1996 ◽  
Vol 45 (1) ◽  
pp. 103-106 ◽  
Author(s):  
Takashi KITAMURA ◽  
Seiji ITO ◽  
Yoshio KATO ◽  
Keiko SASAMOTO ◽  
Mitsuyo OKAZAKI
Keyword(s):  
High Performance ◽  
Gel Filtration ◽  
Serum Lipoproteins ◽  
Gel Filtration Chromatography

Aminoacyl-tRNA synthetases of Halobacterium cutirubrum: preliminary fractionation studies

1970 ◽  
Vol 48 (8) ◽  
pp. 944-946 ◽  
Author(s):  
E. Griffiths
Keyword(s):  
Ionic Strength ◽  
Gel Filtration ◽  
Halophilic Bacterium ◽  
Trna Synthetase ◽  
Partial Purification ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases ◽  
Fractionation Procedure ◽  
The Stability ◽  
Low Ionic Strength

The stability, in solutions of low ionic strength, of aminoacyl-tRNA synthetases from the extremely halophilic bacterium Halobacterium cutirubrum was studied as a preliminary to their fractionation. The enzymes differed considerably in their sensitivity to such solutions. Conditions were found where reactivation from the salt-free and inactive state could be achieved. Removal of both K+ and Mg2+ together generally resulted in better stability than the removal of K+ alone. A low temperature (4°) was also important for stability in buffers of low ionic strength. In some cases the L-amino acid substrates afforded protection against inactivation in the salt-free state. Gel filtration in low ionic strength medium was found to work well as a fractionation procedure; a partial purification of phenylalanyl-tRNA synthetase was effected in this way. The use of other conventional protein fractionation procedures is now possible.

Fractionation of Starch Amylopectin and Amylose by High Performance Gel Filtration Chromatography

1992 ◽  
Vol 44 (2) ◽  
pp. 53-55 ◽  
Author(s):  
J. F. Kennedy ◽  
Z. S. Rivera ◽  
L. L. Lloyd ◽  
F. P. Warner
Keyword(s):  
High Performance ◽  
Gel Filtration ◽  
Gel Filtration Chromatography

scholarly journals Optimization of the Treatment of Wheat Samples for the Determination of Phytic Acid by HPLC with Refractive Index Detection

2009 ◽  
Vol 92 (3) ◽  
pp. 873-878 ◽  
Author(s):  
Rosa Amaro ◽  
Miguel Murillo ◽  
Zurima Gonzlez ◽  
András Escalona ◽  
Lus Hernández
Keyword(s):  
Refractive Index ◽  
High Performance Liquid Chromatography ◽  
Phytic Acid ◽  
High Performance ◽  
Substantial Improvement ◽  
Significant Loss ◽  
Phytic Acid Content ◽  
Extraction Step ◽  
Refractive Index Detection

Abstract The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCl did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91, which is a substantial improvement with respect to values reported by others using this methodology.

Sign in / Sign up

Export Citation Format

Share Document