Determination of epichlorohydrin by sulfite derivatization and ion chromatography: characterization of the sulfite derivatives by ion chromatography–mass spectrometry

2004 ◽  
Vol 1034 (1-2) ◽  
pp. 243-247 ◽  
Author(s):  
Maria Concetta Bruzzoniti ◽  
Samo Andrensek ◽  
Milko Novic ◽  
Daniela Perrachon ◽  
Corrado Sarzanini
Keyword(s):  
Mass Spectrometry ◽  
Ion Chromatography ◽  
Chromatography Mass Spectrometry

Liquid chromatography mass spectrometry based characterization of epitope configurations

2020 ◽  
Vol 12 (45) ◽  
pp. 5476-5484
Author(s):  
Maren Christin Stillesby Levernæs ◽  
Arelí Urtubia Moe ◽  
Sigurd Leinæs Bøe ◽  
Elisabeth Paus ◽  
Léon Reubsaet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Liquid Chromatography Mass Spectrometry ◽  
Three Dimensional Structure ◽  
Chromatography Mass Spectrometry ◽  
Pre Treatment

Here we evaluate a quick and easy tool for determination of epitope configuration using immunocapture and liquid chromatography mass spectrometry (LC-MS) subsequent to pre-treatment of the target protein to disrupt its three-dimensional structure.

scholarly journals Determination of alkyl amines in atmospheric aerosol particles: a comparison of gas chromatography-mass spectrometry and ion chromatography approaches

2014 ◽  
Vol 7 (3) ◽  
pp. 2127-2152 ◽  
Author(s):  
R.-J. Huang ◽  
W.-B. Li ◽  
Y.-R. Wang ◽  
Q. Y. Wang ◽  
K.-F. Ho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ion Chromatography ◽  
Aerosol Particles ◽  
Gas Chromatography Mass Spectrometry ◽  
Limits Of Detection ◽  
Alkyl Amines ◽  
Chromatography Mass Spectrometry ◽  
Analytical Approaches

Abstract. In recent years low molecular weight alkyl amines have been recognized to play an important role in particle formation and growth in the lower atmosphere. However, major uncertainties are associated with their atmospheric processes, sources and sinks, mostly due to the lack of ambient measurements and the difficulties in accurate quantification of alkyl amines at trace level. In this study, we present the evaluation and optimization of two analytical approaches, i.e., gas chromatography-mass spectrometry (GC-MS) and ion chromatography (IC), for the determination of alkyl amines in aerosol particles. Alkyl amines were converted to carbamates through derivatization with isobutyl chloroformate for GC-MS determination. A set of parameters affecting the analytical performances of the GC-MS approach, including reagent amount, reaction time and pH value, was evaluated and optimized. The accuracy is 84.3–99.1%, and the limits of detection obtained are 1.8–3.9 pg. For the IC approach, a solid phase extraction (SPE) column was used to separate alkyl amines from interfering cations before IC analysis. 1–2% (v/v) of acetone (or 2–4% (v/v) of acetonitrile) was added to the eluent to improve the separation of alkyl amines on the IC column. The limits of detection obtained are 2.1–15.9 ng and the accuracy is 55.1–103.4%. The lower accuracy can be attributed to evaporation losses of amines during the sample concentration procedure. Measurements of ambient aerosol particle samples collected in Hong Kong show that the GC-MS approach is superior to the IC approach for the quantification of primary and secondary alkyl amines due to its lower detection limits and higher accuracy.

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