scholarly journals Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels beyond the Optical Diffraction Limit

2012 ◽  
Vol 19 (7) ◽  
pp. 902-912 ◽  
Author(s):  
Alison E. Ondrus ◽  
Hsiao-lu D. Lee ◽  
Shigeki Iwanaga ◽  
William H. Parsons ◽  
Brian M. Andresen ◽  
...  
Keyword(s):  
Ion Channels ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Diffraction Limit ◽  
Live Cell ◽  
Optical Diffraction ◽  
Sodium Ion ◽  
Voltage Gated ◽  
Sodium Ion Channels

scholarly journals Synthesis and Evaluation of Novel α-Aminoamides Containing Benzoheterocyclic Moiety for the Treatment of Pain

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1716
Author(s):  
Kun Tong ◽  
Ruotian Zhang ◽  
Fengzhi Ren ◽  
Tao Zhang ◽  
Junlin He ◽  
...  
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Formalin Test ◽  
Sodium Ion ◽  
Inhibitory Potency ◽  
Voltage Gated ◽  
Patch Clamp Electrophysiology ◽  
Sodium Ion Channels

Novel α-aminoamide derivatives containing different benzoheterocyclics moiety were synthesized and evaluated as voltage-gated sodium ion channels blocks the treatment of pain. Compounds 6a, 6e, and 6f containing the benzofuran group displayed more potent in vivo analgesic activity than ralfinamide in both the formalin test and the writhing assay. Interestingly, they also exhibited potent in vitro anti-Nav1.7 and anti-Nav1.8 activity in the patch-clamp electrophysiology assay. Therefore, compounds 6a, 6e, and 6f, which have inhibitory potency for two pain-related Nav targets, could serve as new leads for the development of analgesic medicines.

scholarly journals Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics

2015 ◽  
Vol 26 (9) ◽  
pp. 1743-1751 ◽  
Author(s):  
Shinichi Hayashi ◽  
Yasushi Okada
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Rotating Disk ◽  
Confocal Microscope ◽  
Diffraction Limit ◽  
Live Cell ◽  
Wide Field ◽  
Structured Illumination ◽  
Stripe Pattern ◽  
Spinning Disk

Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumination microscope (SIM) and achieves a spatial resolution of 120 nm, double that of the diffraction limit of wide-field fluorescence microscopy. However, the SDSRM is 10 times faster than a conventional SIM because SR signals are recovered by optical demodulation through the stripe pattern of the disk. Therefore a single SR image requires only a single averaged image through the rotating disk. On the basis of this theory, we modified a commercial spinning disk confocal microscope. The improved resolution around 120 nm was confirmed with biological samples. The rapid dynamics of micro­tubules, mitochondria, lysosomes, and endosomes were observed with temporal resolutions of 30–100 frames/s. Because our method requires only small optical modifications, it will enable an easy upgrade from an existing spinning disk confocal to a SR microscope for live-cell imaging.

scholarly journals Optical diffraction tomography for high resolution live cell imaging

2009 ◽  
Vol 17 (1) ◽  
pp. 266 ◽  
Author(s):  
Yongjin Sung ◽  
Wonshik Choi ◽  
Christopher Fang-Yen ◽  
Kamran Badizadegan ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

scholarly journals Guanidinium Toxins and Their Interactions with Voltage-Gated Sodium Ion Channels

Marine Drugs ◽  
2017 ◽  
Vol 15 (10) ◽  
pp. 303 ◽  
Author(s):  
Lorena Durán-Riveroll ◽  
Allan Cembella
Keyword(s):  
Ion Channels ◽  
Sodium Ion ◽  
Voltage Gated ◽  
Sodium Ion Channels

Veratridine: A Janus-Faced Modulator of Voltage-Gated Sodium Ion Channels

2020 ◽  
Vol 11 (3) ◽  
pp. 418-426
Author(s):  
Robert A. Craig ◽  
Catherine E. Garrison ◽  
Phuong T. Nguyen ◽  
Vladimir Yarov-Yarovoy ◽  
J. Du Bois
Keyword(s):  
Ion Channels ◽  
Sodium Ion ◽  
Voltage Gated ◽  
Sodium Ion Channels

scholarly journals Optical diffraction tomography for high resolution live cell imaging

2009 ◽  
Author(s):  
Yongjin Sung ◽  
Wonshik Choi ◽  
Christopher Fang-Yen ◽  
Kamran Badizadegan ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Optical Diffraction Tomography

Diversity of Expression of the Sensory Neuron-Specific TTX-Resistant Voltage-Gated Sodium Ion Channels SNS and SNS2

2000 ◽  
Vol 15 (4) ◽  
pp. 331-342 ◽  
Author(s):  
Fumimasa Amaya ◽  
Isabelle Decosterd ◽  
Tarek A. Samad ◽  
Christopher Plumpton ◽  
Simon Tate ◽  
...  
Keyword(s):  
Ion Channels ◽  
Sensory Neuron ◽  
Sodium Ion ◽  
Voltage Gated ◽  
Sodium Ion Channels
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