scholarly journals A Pilot Study of Fecal Serine-Protease Activity: A Pathophysiologic Factor in Diarrhea-Predominant Irritable Bowel Syndrome

2007 ◽  
Vol 5 (5) ◽  
pp. 550-555 ◽  
Author(s):  
Richárd Róka ◽  
András Rosztóczy ◽  
Mathilde Leveque ◽  
Ferenc Izbéki ◽  
Ferenc Nagy ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Pilot Study ◽  
Serine Protease ◽  
Protease Activity ◽  
Serine Protease Activity ◽  
Irritable Bowel

A novel mechanism for imatinib mesylate–induced cell death of BCR-ABL–positive human leukemic cells: caspase-independent, necrosis-like programmed cell death mediated by serine protease activity

Blood ◽  
2004 ◽  
Vol 103 (6) ◽  
pp. 2299-2307 ◽  
Author(s):  
Masayuki Okada ◽  
Souichi Adachi ◽  
Tsuyoshi Imai ◽  
Ken-ichiro Watanabe ◽  
Shin-ya Toyokuni ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Serine Protease ◽  
Imatinib Mesylate ◽  
Protease Activity ◽  
Kinase Inhibitor ◽  
Leukemic Cells ◽  
Endonuclease G ◽  
Serine Protease Activity ◽  
Human Leukemic Cells

Abstract Caspase-independent programmed cell death can exhibit either an apoptosis-like or a necrosis-like morphology. The ABL kinase inhibitor, imatinib mesylate, has been reported to induce apoptosis of BCR-ABL–positive cells in a caspase-dependent fashion. We investigated whether caspases alone were the mediators of imatinib mesylate–induced cell death. In contrast to previous reports, we found that a broad caspase inhibitor, zVAD-fmk, failed to prevent the death of imatinib mesylate–treated BCR-ABL–positive human leukemic cells. Moreover, zVAD-fmk–preincubated, imatinib mesylate–treated cells exhibited a necrosis-like morphology characterized by cellular pyknosis, cytoplasmic vacuolization, and the absence of nuclear signs of apoptosis. These cells manifested a loss of the mitochondrial transmembrane potential, indicating the mitochondrial involvement in this caspase-independent necrosis. We excluded the participation of several mitochondrial factors possibly involved in caspase-independent cell death such as apoptosis-inducing factor, endonuclease G, and reactive oxygen species. However, we observed the mitochondrial release of the serine protease Omi/HtrA2 into the cytosol of the cells treated with imatinib mesylate or zVAD-fmk plus imatinib mesylate. Furthermore, serine protease inhibitors prevented the caspase-independent necrosis. Taken together, our results suggest that imatinib mesylate induces a caspase-independent, necrosis-like programmed cell death mediated by the serine protease activity of Omi/HtrA2.

Acute myocardial infarction elevates serine protease activity in saliva of patients with periodontitis

2011 ◽  
Vol 47 (3) ◽  
pp. 345-353 ◽  
Author(s):  
P. Mäntylä ◽  
E. Buduneli ◽  
G. Emingil ◽  
T. Tervahartiala ◽  
P. J. Pussinen ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Serine Protease ◽  
Protease Activity ◽  
Serine Protease Activity

Human Monocytes Possess a Serine Protease Activity Capable of Degrading HIV-1 Reverse Transcriptase in Vitro

2001 ◽  
Vol 285 (4) ◽  
pp. 863-872 ◽  
Author(s):  
Marie-Thérèse Château ◽  
Véronique Robert-Hebmann ◽  
Christian Devaux ◽  
Jean-Bernard Lazaro ◽  
Bruno Canard ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Serine Protease ◽  
Protease Activity ◽  
Human Monocytes ◽  
Serine Protease Activity ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document