Cloning, expression and activity optimization of trehalose synthase from Thermus thermophilus HB27

2015 ◽  
Vol 135 ◽  
pp. 323-329 ◽  
Author(s):  
Yan Li ◽  
Xinxiao Sun ◽  
Yue Feng ◽  
Qipeng Yuan
Keyword(s):  
Thermus Thermophilus ◽  
Trehalose Synthase ◽  
Activity Optimization ◽  
Expression And Activity

scholarly journals Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1087 ◽  
Author(s):  
Jing Sun ◽  
Shizeng Wang ◽  
Wenna Li ◽  
Ruimin Li ◽  
Sheng Chen ◽  
...  
Keyword(s):  
Thermus Thermophilus ◽  
Trehalose Synthase

scholarly journals Osmotic Adaptation of Thermus thermophilus RQ-1: Lesson from a Mutant Deficient in Synthesis of Trehalose

2003 ◽  
Vol 185 (20) ◽  
pp. 5943-5952 ◽  
Author(s):  
Zélia Silva ◽  
Susana Alarico ◽  
Ana Nobre ◽  
Reinhold Horlacher ◽  
Joey Marugg ◽  
...  
Keyword(s):  
Salt Stress ◽  
Yeast Extract ◽  
Thermus Thermophilus ◽  
Gene Replacement ◽  
Defined Medium ◽  
Growth Media ◽  
Trehalose Synthase ◽  
Osmotic Adaptation ◽  
Dna Fragment

ABSTRACT Strains of Thermus thermophilus accumulate primarily trehalose and smaller amounts of mannosylglycerate in response to salt stress in yeast extract-containing media (O. C. Nunes, C. M. Manaia, M. S. da Costa, and H. Santos, Appl. Environ. Microbiol. 61:2351-2357, 1995). A 2.4-kbp DNA fragment from T. thermophilus strain RQ-1 carrying otsA (encoding trehalose-phosphate synthase [TPS]), otsB (encoding trehalose-phosphate phosphatase [TPP]), and a short sequence of the 5′ end of treS (trehalose synthase [TreS]) was cloned from a gene library. The sequences of the three genes (including treS) were amplified by PCR and sequenced, revealing that the genes were structurally linked. To understand the role of trehalose during salt stress in T. thermophilus RQ-1, we constructed a mutant, designated RQ-1M6, in which TPS (otsA) and TPP (otsB) genes were disrupted by gene replacement. Mutant RQ-1M6 accumulated trehalose and mannosylglycerate in a medium containing yeast extract and NaCl. However, growth in a defined medium (without yeast extract, known to contain trehalose) containing NaCl led to the accumulation of mannosylglycerate but not trehalose. The deletion of otsA and otsB reduced the ability to grow in defined salt-containing medium, with the maximum salinity being 5% NaCl for RQ-1 and 3% NaCl for RQ-1M6. The lower salt tolerance observed in the mutant was relieved by the addition of trehalose to the growth media. In contrast to trehalose, the addition of glycine betaine, mannosylglycerate, maltose, and glucose to the growth medium did not allow the mutant to grow at higher salinities. The results presented here provide crucial evidence for the importance of the TPS/TPP pathway for the synthesis and accumulation of trehalose and the decisive contribution of this disaccharide to osmotic adaptation in T. thermophilus RQ-1.

1060: Tadalafil does not Up-Regulate the Expression and Activity of Phosphodiesterase 5 (PDE-5) in the Penis

2005 ◽  
Vol 173 (4S) ◽  
pp. 287-288
Author(s):  
Dolores Vemet ◽  
Thomas R. Magee ◽  
Ansha Qian ◽  
Gaby Nolazco ◽  
Jacob Rajfer ◽  
...  
Keyword(s):  
Phosphodiesterase 5 ◽  
Expression And Activity

RAGE modulates neuronal glyoxalase-1 transcription, expression and activity

2006 ◽  
Vol 1 (S 1) ◽  
Author(s):  
SB Stoyanov ◽  
GM Haag ◽  
I Konrade ◽  
IK Lukic ◽  
P Humpert ◽  
...  
Keyword(s):  
Glyoxalase 1 ◽  
Transcription Expression ◽  
Expression And Activity

Diminished Ca2+Signaling, CaV1 Channel Expression, and Activity in Pancreatic Beta Cells Expressing Mutated DISC1 (Disrupted in Schizophrenia 1)

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2163-P
Author(s):  
PING LU ◽  
ROHIT B. SHARMA ◽  
LAURA C. ALONSO ◽  
RONGHUA ZHUGE ◽  
ANN R. RITTENHOUSE ◽  
...  
Keyword(s):  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Channel Expression ◽  
Expression And Activity
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