scholarly journals Propionic Acid Promotes the Virulent Phenotype of Crohn’s Disease-Associated Adherent-Invasive Escherichia coli

Cell Reports ◽  
2020 ◽  
Vol 30 (7) ◽  
pp. 2297-2305.e5 ◽  
Author(s):  
Michael J. Ormsby ◽  
Síle A. Johnson ◽  
Nuria Carpena ◽  
Lynsey M. Meikle ◽  
Robert J. Goldstone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Propionic Acid ◽  
Virulent Phenotype

scholarly journals Propionic acid promotes the virulent phenotype of Crohn’s disease-associated adherent-invasive Escherichia coli

2018 ◽  
Author(s):  
Michael J. Ormsby ◽  
Síle A. Johnson ◽  
Lynsey M. Meikle ◽  
Robert J. Goldstone ◽  
Anne McIntosh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Propionic Acid ◽  
Biofilm Formation ◽  
Food Production ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Phenotypic Changes

AbstractThe short chain fatty acid propionic acid (PA) is a bacteria-derived human intestinal antimicrobial and immune modulator used widely in Western food production and agriculture. Here we examine the effect of PA on the pathogenicity of the Crohn’s disease-associated microbe, adherent-invasive Escherichia coli (AIEC). Passage of AIEC through a murine model, where the low intestinal PA levels were increased to replicate those of the human intestine, led to the recovery of AIEC post-infection that had significantly increased virulence. These phenotypic changes, including increased adhesion to intestinal epithelial cells and biofilm formation, could be replicated in AIEC in vitro through exposure to PA alone. This in vitro exposure of AIEC to PA fundamentally changed AIEC virulence, with strains exposed to PA in vitro subsequently persisting at 20-fold higher levels in a murine model compared to non-exposed strains. RNA-sequencing identified the transcriptional changes in AIEC in response to PA with upregulation of genes involved in biofilm formation, stress responses, metabolism, membrane integrity and alternative carbon source utilisation. These PA induced changes in virulence could be replicated in a number of E. coli isolates from Crohn’s disease patients. Finally, removal of the PA selective pressure was sufficient to reverse these phenotypic changes. Our data indicate that exposure of AIEC to PA evolves bacteria that are both resistant to this natural human intestinal antimicrobial and increasingly virulent in its presence.ImportanceExposure to propionic acid, an intestinal short chain fatty acid and commonly used antimicrobial in Western food production, induces significant virulence associated phenotypic changes in adherent-invasive Escherichia coli (AIEC).

Sa536 GENOME DIVERSITY OF INTESTINAL ESCHERICHIA COLI STRAINS FROM PATIENTS WITH CROHN'S DISEASE AND HEALTHY INDIVIDUALS

2021 ◽  
Vol 160 (6) ◽  
pp. S-539
Author(s):  
Maria Siniagina ◽  
Maria Markelova ◽  
Alexander Laikov ◽  
Dilyara Khusnutdinova ◽  
Eugenia A. Boulygina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Healthy Individuals ◽  
Genome Diversity

scholarly journals Differentiation of Crohn’s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force

Biology ◽  
2016 ◽  
Vol 5 (2) ◽  
pp. 14 ◽  
Author(s):  
Sabine Szunerits ◽  
Oleksandr Zagorodko ◽  
Virginie Cogez ◽  
Tetiana Dumych ◽  
Thibaut Chalopin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Shear Force ◽  
Pathogenic Escherichia Coli

Mo1770 GipA Factor Supports Crohn's Disease-Associated Escherichia coli Growth in Peyer's Patches by Providing Resistance to Bactericidal Stress Encountered in Macrophages

2015 ◽  
Vol 148 (4) ◽  
pp. S-707
Author(s):  
Emilie Vazeille ◽  
Benoit Chassaing ◽  
Anthony Buisson ◽  
Anaëlle Dubois ◽  
Amélie De Vallée ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Coli Growth

scholarly journals Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Timothy R. Elliott ◽  
Neil B. Rayment ◽  
Barry N. Hudspith ◽  
Rebecca E. Hands ◽  
Kirstin Taylor ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Lamina Propria ◽  
Macrophage Phenotypes

PROTEOMIC PROFILING OF ESCHERICHIA COLI, ISOLATED FROM CROHN’S DISEASE PATIENTS

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
D.S. Matyushkina ◽  
O.V. Pobeguts ◽  
O.N. Bukato ◽  
D.V. Rakitina ◽  
Yu.P. Baikova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Proteomic Profiling
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