scholarly journals A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production

Cell Reports ◽  
2019 ◽  
Vol 29 (10) ◽  
pp. 2970-2978.e6 ◽  
Author(s):  
Anastasia Hyrina ◽  
Christopher Jones ◽  
Darlene Chen ◽  
Scott Clarkson ◽  
Nadire Cochran ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Host Factor ◽  
Antigen Production ◽  
Genome Wide ◽  
A Genome ◽  
Crispr Screen

scholarly journals A genome-wide CRISPR screen identifies ZCCHC14 as a host factor required for hepatitis B surface antigen production

2019 ◽  
Author(s):  
Anastasia Hyrina ◽  
Christopher Jones ◽  
Darlene Chen ◽  
Scott Clarkson ◽  
Nadire Cochran ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Genome Wide ◽  
A Genome ◽  
Chronic Hepatitis B Virus ◽  
B Virus ◽  
Crispr Screen ◽  
Novel Host ◽  
Circulating Levels

GRAPHICAL ABSTRACTSUMMARYA hallmark of chronic hepatitis B virus (CHB) infection is the presence of high circulating levels of non-infectious small lipid HBV surface antigen (HBsAg) vesicles. Although rare, sustained HBsAg loss is the idealized endpoint of any CHB therapy. A novel small molecule RG7834 has been previously reported to inhibit HBsAg expression by targeting terminal nucleotidyltransferase protein 4A and 4B (TENT4A and TENT4B). In this study, we describe a genome-wide CRISPR screen to identify other potential novel host factors required for HBsAg expression and to gain further insights into the mechanism of RG7834. We report more than 60 genes involved in regulating HBsAg and identified novel factors involved in RG7834 activity, including a zinc finger CCHC-type containing 14 (ZCCHC14) protein. We show that ZCCHC14, together with TENT4A/B, stabilizes HBsAg expression through HBV RNA tailing, providing a potential new therapeutic target to achieve functional cure in CHB patients.

scholarly journals Alisporivir Inhibition of Hepatocyte Cyclophilins Reduces HBV Replication and Hepatitis B Surface Antigen Production

2015 ◽  
Vol 148 (2) ◽  
pp. 403-414.e7 ◽  
Author(s):  
Sandra Phillips ◽  
Shilpa Chokshi ◽  
Udayan Chatterji ◽  
Antonio Riva ◽  
Michael Bobardt ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigen Production ◽  
Hbv Replication

Excessive Hepatitis-B Surface Antigen Production After Corticosteroids and the Development of Immunoblastic Lymphoma

1993 ◽  
Vol 10 (3) ◽  
pp. 241-244 ◽  
Author(s):  
Eyal Ras ◽  
Joseph Michaeli ◽  
Eliezer Rosenmann ◽  
Mordecal M. Popovtzer ◽  
Aaron Polliack ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigen Production ◽  
Immunoblastic Lymphoma
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