scholarly journals Plexin B2 Is a Regulator of Monocyte Apoptotic Cell Disassembly

Cell Reports ◽  
2019 ◽  
Vol 29 (7) ◽  
pp. 1821-1831.e3 ◽  
Author(s):  
Georgia K. Atkin-Smith ◽  
Mark A. Miles ◽  
Rochelle Tixeira ◽  
Fung T. Lay ◽  
Mubing Duan ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Apoptotic Cell Disassembly

scholarly journals Bracovirus sneaks into apoptotic bodies transmitting immunosuppressive signaling driven by integration-mediated eIF5A hypusination

2021 ◽  
Author(s):  
Kaijun Luo
Keyword(s):  
Extracellular Vesicles ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Apoptotic Cell ◽  
Recipient Cell ◽  
Nucleocytoplasmic Transport ◽  
Host Cells ◽  
Repair System ◽  
Apoptotic Cell Disassembly ◽  
Cellular Apoptosis

Abstract The typical characteristic of polydnavirus (PDV) infection is persistent immunosuppression, governed by the viral integration and expression of virulence genes. Recently, how Microplitis bicoloratus bracovirus (MbBV) activates caspase-3 to cleave Innexins, gap junction proteins, has been highlighted, further promoting apoptotic cell disassembly and apoptotic body (AB) formation. However, little is known about how inserted viral fragments interact with host cells to drive apoptosis and the role MbBV-mediated extracellular vesicles play in immune suppression. Herein, we show that ABs transmitted immunosuppressive signaling, causing recipient cell apoptosis and dismigration. Overall, viral host integrated motif sites insertion damaged host genome stimulating eIF5A nucleocytoplasmic transport, activating the eIF5A-hypusination translation pathway. Functionally, translated apoptosis-related host proteins, such as P53, CypD, CypJ, and CypA, drive a broken dsDNA cellular apoptosis. Furthermore, translated viral proteins, vank86, 92, and 101, known to complex with transcription factor Dip3, positively regulated DHYS and DOHH transcription maintaining the activation of the eIF5A-hypusination translation pathway. Mechanistically, MbBV-mediated extracellular vesicles contained inserted viral fragments that re-integrated into recipients, potentially via the homologous recombinant repair system. Meanwhile, this stimulation regulated activated caspase-3 level via pI3K/Akt 308 and 473 dephosphorylation to promote recipients cell apoptosis; pI3K/Akt 473 phosphorylation inhibited caspase-3 activation leading to recipient cell dismigration. In conclusion, our results suggest that integration-mediated eIF5A hypusination drives extracellular vesicles for continues immunosuppressive.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

Author response for "Soluble CD93 is an apoptotic cell opsonin recognized by α x β 2"

2018 ◽  
Author(s):  
Jack W. D. Blackburn ◽  
Darius H. C. Lau ◽  
Elaine Y. Liu ◽  
Jessica Ellins ◽  
Angela M. Vrieze ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Author Response

KIOM-79 prevents apoptotic cell death and AGEs accumulation in the retina of diabetic db/db mice

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
YS Kim ◽  
EJ Sohn ◽  
HY Lee ◽  
CS Kim ◽  
YM Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death
Sign in / Sign up

Export Citation Format

Share Document