scholarly journals Single-Cell Chromatin Analysis of Mammary Gland Development Reveals Cell-State Transcriptional Regulators and Lineage Relationships

Cell Reports ◽  
2019 ◽  
Vol 29 (2) ◽  
pp. 495-510.e6 ◽  
Author(s):  
Chi-Yeh Chung ◽  
Zhibo Ma ◽  
Christopher Dravis ◽  
Sebastian Preissl ◽  
Olivier Poirion ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Single Cell ◽  
Mammary Gland Development ◽  
Transcriptional Regulators ◽  
Cell State ◽  
Gland Development

scholarly journals Single-Cell Transcriptomes Distinguish Stem Cell State Changes and Lineage Specification Programs in Early Mammary Gland Development

Cell Reports ◽  
2018 ◽  
Vol 24 (6) ◽  
pp. 1653-1666.e7 ◽  
Author(s):  
Rajshekhar R. Giraddi ◽  
Chi-Yeh Chung ◽  
Richard E. Heinz ◽  
Ozlen Balcioglu ◽  
Mark Novotny ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mammary Gland ◽  
Single Cell ◽  
Mammary Gland Development ◽  
Lineage Specification ◽  
Cell State ◽  
State Changes ◽  
Gland Development ◽  
Stem Cell State

scholarly journals Single-cell chromatin accessibility analysis of mammary gland development reveals cell state transcriptional regulators and cellular lineage relationships

2019 ◽  
Author(s):  
Chi-Yeh Chung ◽  
Zhibo Ma ◽  
Christopher Dravis ◽  
Sebastian Preissl ◽  
Olivier Poirion ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Single Cell ◽  
Web Application ◽  
Mammary Gland Development ◽  
Transcriptional Regulators ◽  
Chromatin Accessibility ◽  
Mammary Development ◽  
Normal Mammary Gland ◽  
Chromatin Profiling ◽  
Gland Development

SummaryIt has only recently become possible to obtain single-cell level resolution of the epigenetic changes that occur during organ development. We reasoned that precision single-cell chromatin accessibility mapping of mammary gland development could provide needed insight into the epigenetic reprogramming and transcriptional regulators involved in normal mammary gland development. Here, we provide the first single-cell resource of chromatin accessibility for murine mammary development from the peak of fetal mammary stem cell (fMaSC) functional activity in late embryogenesis to the differentiation of adult basal and luminal cells. We find that the chromatin landscape within individual cells predicts both gene accessibility and transcription factor activity, and we present a web application as a scientific resource for facilitating future analyses. Strikingly, these single-cell chromatin profiling data reveal that fMaSCs can be separated into basal-like and luminal-like lineages, providing evidence of early lineage segregation prior to birth. Such distinctions were not evident in analyses of single-cell transcriptomic data.

scholarly journals Epigenetic and Transcriptomic Profiling of Mammary Gland Development and Tumor Models Disclose Regulators of Cell State Plasticity

Cancer Cell ◽  
2018 ◽  
Vol 34 (3) ◽  
pp. 466-482.e6 ◽  
Author(s):  
Christopher Dravis ◽  
Chi-Yeh Chung ◽  
Nikki K. Lytle ◽  
Jaslem Herrera-Valdez ◽  
Gidsela Luna ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Tumor Models ◽  
Transcriptomic Profiling ◽  
Cell State ◽  
Gland Development
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