scholarly journals Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic

Cell Reports ◽  
2019 ◽  
Vol 27 (8) ◽  
pp. 2459-2467.e3 ◽  
Author(s):  
Brittany Jack ◽  
David M. Mueller ◽  
Ann C. Fee ◽  
Ashley L. Tetlow ◽  
Prachee Avasthi
Keyword(s):  
Transition Zone ◽  
Actin Genes

scholarly journals Partially redundant actin genes in Chlamydomonas control flagellum-directed traffic and transition zone organization

2017 ◽  
Author(s):  
Brittany Jack ◽  
David M. Mueller ◽  
Ann C. Fee ◽  
Ashley Tetlow ◽  
Prachee Avasthi
Keyword(s):  
Transition Zone ◽  
Mammalian Cells ◽  
Intraflagellar Transport ◽  
Actin Genes ◽  
Transmission Electron ◽  
Unicellular Green Alga ◽  
Absolute Requirement ◽  
Flagellar Assembly ◽  
Flagellar Proteins ◽  
Flagellar Biogenesis

ABSTRACTFlagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of mammalian cells and provide an excellent model to study ciliogenesis. These biflagellated cells have two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1). Previously, we described a role for actin in the regulation of flagella-building intraflagellar transport machinery. Here, we probe how actin redundancy contributes to this process using a nap1 mutant Chlamydomonas strain. Disruption of a single actin allows normal or slower incorporation but complete flagellar assembly. However, when we disrupt both actins using Latrunculin B (LatB) treatment on the nap1 mutant background, we find flagellar growth from newly synthesized limiting flagellar proteins is actin-dependent. Upon total actin disruption during flagellar assembly, transmission electron microscopy identified an accumulation of Golgi-adjacent vesicles, suggesting impaired vesicular trafficking may be the mechanism by which actin supports flagellar growth from new flagellar proteins. We also find there is a mislocalization of a key transition zone gating and ciliopathy protein, NPHP-4. Extended (2 hour) treatment with LatB, a condition under which NAP1 is upregulated, restores NPHP-4 localization. This suggests NAP1 can perform the functions of conventional actin at the transition zone. Our experiments demonstrate that each stage of flagellar biogenesis requires redundant actin function to varying degrees, with an absolute requirement for these actins in transport of Golgi-adjacent vesicles and flagellar incorporation of newly synthesized proteins.

Theoretical modeling of the regular olivine intergrowths in the mimetic paramorphosis after ringwoodite and wadsleyite

2018 ◽  
pp. 3-12
Author(s):  
B. B. Shkursky
Keyword(s):  
Transition Zone ◽  
Theoretical Modeling ◽  
Mantle Transition Zone ◽  
Misorientation Angles

Theoretical modeling of regular olivine grains misorientations in mimetic paramorphoses after ringwoodite and wadsleyite, the formation of which during the ascension of matter from the Mantle Transition Zone is expected, has been carried out. The coordinates of the misorientation axes and the misorientation angles, characterizing 10 operations of alignment in the pair intergrowths of olivine grains, eight of which are twins, are calculated. Possible conditions for the formation of mimetic paramorphoses predicted here, and the chances of their persistence are discussed. The calculated orientations are compared with the known twinning laws of olivine.

Mathematical Model of the Effective Properties of a Fiber Reinforced Composite with a Linearly Graded Transition Zone

2010 ◽  
Vol 38 (4) ◽  
pp. 286-307
Author(s):  
Carey F. Childers
Keyword(s):  
Mathematical Model ◽  
Transition Zone ◽  
Effective Properties ◽  
Local Stress ◽  
Stress And Strain ◽  
Fiber Reinforced ◽  
Strain Fields ◽  
Shear Moduli ◽  
Fiber Reinforced Composite ◽  
Reinforced Composite

Abstract Tires are fabricated using single ply fiber reinforced composite materials, which consist of a set of aligned stiff fibers of steel material embedded in a softer matrix of rubber material. The main goal is to develop a mathematical model to determine the local stress and strain fields for this isotropic fiber and matrix separated by a linearly graded transition zone. This model will then yield expressions for the internal stress and strain fields surrounding a single fiber. The fields will be obtained when radial, axial, and shear loads are applied. The composite is then homogenized to determine its effective mechanical properties—elastic moduli, Poisson ratios, and shear moduli. The model allows for analysis of how composites interact in order to design composites which gain full advantage of their properties.

Lithostratigraphy of the Kweekvlei Formation (Witteberg Group), Cape Supergroup

2017 ◽  
Vol 120 (3) ◽  
pp. 421-432 ◽  
Author(s):  
C. Browning ◽  
M. Reid
Keyword(s):  
South Africa ◽  
Transition Zone ◽  
Distal Part ◽  
Vascular Plant ◽  
Trace Fossil ◽  
Plant Debris ◽  
Lower Carboniferous ◽  
Fossil Assemblage ◽  
Low Diversity ◽  
Storm Wave

AbstractThe Lower Carboniferous, probably Tournaisian, Kweekvlei Formation is part of the Witteberg Group (Cape Supergroup) of South Africa. Together with the overlying Floriskraal Formation, it forms an upward-coarsening succession within the Lake Mentz Subgroup. Sedimentary features of the Kweekvlei Formation suggest deposition in a storm-wave dominated marine setting, within the storm-influenced, distal part of an offshore transition zone environment. This predominantly argillaceous formation preserves a low diversity trace fossil assemblage. Reworked vascular plant debris (including the problematic genus Praeramunculus sp.) and a shark spine have been reported for the Kweekvlei Formation. There are no known stratigraphic equivalents in South Africa.

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