scholarly journals A Role for the Insulin Receptor in the Suppression of Dengue Virus and Zika Virus in Wolbachia-Infected Mosquito Cells

Cell Reports ◽  
2019 ◽  
Vol 26 (3) ◽  
pp. 529-535.e3 ◽  
Author(s):  
Gholamreza Haqshenas ◽  
Gerard Terradas ◽  
Prasad N. Paradkar ◽  
Jean-Bernard Duchemin ◽  
Elizabeth A. McGraw ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Insulin Receptor ◽  
Zika Virus ◽  
Infected Mosquito ◽  
Mosquito Cells

scholarly journals Ultrastructural Characterization and Three-Dimensional Architecture of Replication Sites in Dengue Virus-Infected Mosquito Cells

2014 ◽  
Vol 88 (9) ◽  
pp. 4687-4697 ◽  
Author(s):  
J. Junjhon ◽  
J. G. Pennington ◽  
T. J. Edwards ◽  
R. Perera ◽  
J. Lanman ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Three Dimensional ◽  
Infected Mosquito ◽  
Mosquito Cells ◽  
Ultrastructural Characterization ◽  
Replication Sites

scholarly journals Secretion of Nonstructural Protein 1 of Dengue Virus from Infected Mosquito Cells: Facts and Speculations

2018 ◽  
Vol 92 (14) ◽  
Author(s):  
Ana C. Alcalá ◽  
Laura A. Palomares ◽  
Juan E. Ludert
Keyword(s):  
Dengue Virus ◽  
Nonstructural Protein ◽  
Infected Mosquito ◽  
Nonstructural Protein 1 ◽  
Mosquito Cells

ABSTRACTDengue virus nonstructural protein 1 (NS1) is a multifunctional glycoprotein. For decades, the notion in the field was that NS1 is secreted exclusively from vertebrate cells and not from mosquito cells. However, recent evidence shows that mosquito cells also secrete NS1 efficiently. In this review, we discuss the evidence for secretion of NS1 of dengue virus, and of other flaviviruses, from mosquito cells, differences between NS1 secreted from mosquito and NS1 secreted from vertebrate cells, and possible roles of soluble NS1 in the insect flavivirus vector.

scholarly journals The dengue virus non-structural protein 1 (NS1) is secreted from infected mosquito cells via a non-classical caveolin-1-dependent pathway

2017 ◽  
Vol 98 (8) ◽  
pp. 2088-2099 ◽  
Author(s):  
Ana C. Alcalá ◽  
Raiza Hernández-Bravo ◽  
Fernando Medina ◽  
David S. Coll ◽  
Jose L. Zambrano ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Infected Mosquito ◽  
Structural Protein ◽  
Caveolin 1 ◽  
Mosquito Cells ◽  
Dependent Pathway

scholarly journals The dengue virus non-structural protein 1 (NS1) is secreted efficiently from infected mosquito cells

Virology ◽  
2016 ◽  
Vol 488 ◽  
pp. 278-287 ◽  
Author(s):  
Ana C. Alcalá ◽  
Fernando Medina ◽  
Arturo González-Robles ◽  
Lizbeth Salazar-Villatoro ◽  
Rogelio J. Fragoso-Soriano ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Infected Mosquito ◽  
Structural Protein ◽  
Mosquito Cells

scholarly journals Life as a Vector of Dengue Virus: The Antioxidant Strategy of Mosquito Cells to Survive Viral Infection

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 395
Author(s):  
Chih-Chieh Cheng ◽  
Eny Sofiyatun ◽  
Wei-June Chen ◽  
Lian-Chen Wang
Keyword(s):  
Dengue Virus ◽  
Viral Infection ◽  
Denv Infection ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Infected Mosquito ◽  
Mosquito Vector ◽  
Evolutionary Pathway ◽  
Antioxidant Genes ◽  
Mosquito Cells

Dengue fever is a mosquito-borne viral disease of increasing global importance. The disease has caused heavy burdens due to frequent outbreaks in tropical and subtropical areas of the world. The dengue virus (DENV) is generally transmitted between human hosts via the bite of a mosquito vector, primarily Aedes aegypti and Ae. albopictus as a minor species. It is known that the virus needs to alternately infect mosquito and human cells. DENV-induced cell death is relevant to the pathogenesis in humans as infected cells undergo apoptosis. In contrast, mosquito cells mostly survive the infection; this allows infected mosquitoes to remain healthy enough to serve as an efficient vector in nature. Overexpression of antioxidant genes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutaredoxin (Grx), thioredoxin (Trx), and protein disulfide isomerase (PDI) have been detected in DENV2-infected mosquito cells. Additional antioxidants, including GST, eukaryotic translation initiation factor 5A (eIF5a), and p53 isoform 2 (p53-2), and perhaps some others, are also involved in creating an intracellular environment suitable for cell replication and viral infection. Antiapoptotic effects involving inhibitor of apoptosis (IAP) upregulation and subsequent elevation of caspase-9 and caspase-3 activities also play crucial roles in the ability of mosquito cells to survive DENV infection. This article focused on the effects of intracellular responses in mosquito cells to infection primarily by DENVs. It may provide more information to better understand virus/cell interactions that can possibly elucidate the evolutionary pathway that led to the mosquito becoming a vector.

scholarly journals Monitoring Mitochondrial Function in Aedes albopictus C6/36 Cell Line during Dengue Virus Infection

Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 934
Author(s):  
María E. Santana-Román ◽  
Paola Maycotte ◽  
Salvador Uribe-Carvajal ◽  
Cristina Uribe-Alvarez ◽  
Nayeli Alvarado-Medina ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Cell Line ◽  
Aedes Albopictus ◽  
Mitochondrial Function ◽  
Mitochondrial Fission ◽  
Hemorrhagic Fever ◽  
Denv Infection ◽  
Infected Mosquito ◽  
Mosquito Cells ◽  
Induced Changes

Aedes aegypti and Aedes albopictus mosquitoes are responsible for dengue virus (DENV) transmission in tropical and subtropical areas worldwide, where an estimated 3 billion people live at risk of DENV exposure. DENV-infected individuals show symptoms ranging from sub-clinical or mild to hemorrhagic fever. Infected mosquitoes do not show detectable signs of disease, even though the virus maintains a lifelong persistent infection. The interactions between viruses and host mitochondria are crucial for virus replication and pathogenicity. DENV infection in vertebrate cells modulates mitochondrial function and dynamics to facilitate viral proliferation. Here, we describe that DENV also regulates mitochondrial function and morphology in infected C6/36 mosquito cells (derived from Aedes albopictus). Our results showed that DENV infection increased ROS (reactive oxygen species) production, modulated mitochondrial transmembrane potential and induced changes in mitochondrial respiration. Furthermore, we offer the first evidence that DENV causes translocation of mitofusins to mitochondria in the C6/36 mosquito cell line. Another protein Drp-1 (Dynamin-related protein 1) did not localize to mitochondria in DENV-infected cells. This observation therefore ruled out the possibility that the abovementioned alterations in mitochondrial function are associated with mitochondrial fission. In summary, this report provides some key insights into the virus–mitochondria crosstalk in DENV infected mosquito cells.

Enoxacin shows a broad-spectrum antiviral activity against diverse viruses by enhancing antiviral RNAi in insects

2021 ◽  
Author(s):  
Bao Lyu ◽  
Chang Wang ◽  
Yuanyuan Bie ◽  
Jing Kong ◽  
An Wang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Viral Replication ◽  
Broad Spectrum ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Fruit Flies ◽  
Dependent Manner ◽  
Mosquito Cells ◽  
Infected Cells ◽  
Rnai Response

RNA interference (RNAi) functions as the major host antiviral defense in insects, while less is understood about how to utilize antiviral RNAi in controlling viral infection in insects. Enoxacin belongs to the family of synthetic antibacterial compounds based on a fluoroquinolone skeleton that has been previously found to enhance RNAi in mammalian cells. In this study, we showed that enoxacin efficiently inhibited viral replication of Drosophila C virus (DCV) and Cricket paralysis virus (CrPV) in cultured Drosophila cells. Enoxacin promoted the loading of Dicer-2-processed virus-derived siRNA into the RNA-induced silencing complex, thereby enhancing antiviral RNAi response in infected cells. Moreover, enoxacin treatment elicited an RNAi-dependent in vivo protective efficacy against DCV or CrPV challenge in adult fruit flies. In addition, enoxacin also inhibited replication of flaviviruses, including Dengue virus and Zika virus, in Aedes mosquito cells in an RNAi-dependent manner. Together, our findings demonstrated that enoxacin can enhance RNAi in insects, and enhancing RNAi by enoxacin is an effective antiviral strategy against diverse viruses in insects, which may be exploited as a broad-spectrum antiviral agent to control vector transmission of arboviruses or viral diseases in insect farming. Importance RNAi has been widely recognized as one of the most broadly acting and robust antiviral mechanism in insects. However, the application of antiviral RNAi in controlling viral infections in insects is less understood. Enoxacin is a fluoroquinolone compound that has been previously found to enhance RNAi in mammalian cells, while its RNAi-enhancing activity has not been assessed in insects. Herein, we showed that enoxacin treatment inhibited viral replication of DCV and CrPV in Drosophila cells and in adult fruit flies. Enoxacin promoted the loading of Dicer-generated virus-derived siRNA into Ago2-incorporated RNA-induced silencing complex, and in turn strengthened the antiviral RNAi response in the infected cells. Moreover, enoxacin also displayed effective RNAi-dependent antiviral effects against flaviviruses, such as Dengue virus and Zika virus, in mosquito cells. This study is the first to demonstrate that enhancing RNAi by enoxacin elicits potent antiviral efficacies against diverse viruses in insects.

scholarly journals Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1105
Author(s):  
Lie Cheng ◽  
Wei-Liang Liu ◽  
Hsing-Han Li ◽  
Matthew P. Su ◽  
Shih-Cheng Wu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Mosquito Control ◽  
Detection Threshold ◽  
Disease Outbreaks ◽  
Rapid Test ◽  
Infected Mosquito ◽  
Mosquito Cells ◽  
Crucial Component ◽  
Sensitivity Tests ◽  
Triton X

Dengue virus (DENV), the pathogen that causes dengue fever, is mainly transmitted by Aedes aegypti. Surveillance of infected mosquitoes is a major component of integrated mosquito control methods for reducing the risk of vector-born disease outbreaks. However, a specialized rapid test for DENV detection in mosquitoes is not currently available. Utilizing immunoblotting, we found that the secretion of NS1 from both a DENV-infected mosquito cell line and mosquito bodies was below the detection threshold. However, when Triton X-100 was used to lyse infected mosquitoes, intracellular NS1 was released, and could then be effectively detected by the NS1 rapid test. The distribution of DENV NS1 in intrathoracically infected mosquitoes was different from that of orally infected mosquitoes. Next, we performed sensitivity tests by bisecting mosquitoes longitudinally; one half of each mosquito was subjected to the NS1 rapid test while the other half was used for qPCR confirmation. This modified test had a sensitivity of nearly 90% from five days post-infection onwards, while DENV had escaped from the midgut barrier. This adapted test offers a valuable, easy-to-use tool for mosquito surveillance, which is a crucial component of DENV disease control.

scholarly journals Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells

2012 ◽  
Vol 8 (3) ◽  
pp. e1002584 ◽  
Author(s):  
Rushika Perera ◽  
Catherine Riley ◽  
Giorgis Isaac ◽  
Amber S. Hopf-Jannasch ◽  
Ronald J. Moore ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Infected Mosquito ◽  
Lipid Homeostasis ◽  
Dengue Virus Infection ◽  
Mosquito Cells
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