scholarly journals Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint

Cell Reports ◽  
2018 ◽  
Vol 25 (9) ◽  
pp. 2317-2328.e5 ◽  
Author(s):  
Thomas Wild ◽  
Magda Budzowska ◽  
Susanne Hellmuth ◽  
Susana Eibes ◽  
Gopal Karemore ◽  
...  
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells

scholarly journals BUB1 Is Essential for the Viability of Human Cells in which the Spindle Assembly Checkpoint Is Compromised

Cell Reports ◽  
2018 ◽  
Vol 22 (6) ◽  
pp. 1424-1438 ◽  
Author(s):  
Jonne A. Raaijmakers ◽  
Roy G.H.P. van Heesbeen ◽  
Vincent A. Blomen ◽  
Louise M.E. Janssen ◽  
Ferdy van Diemen ◽  
...  
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells

scholarly journals The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity

Cell Reports ◽  
2016 ◽  
Vol 14 (8) ◽  
pp. 1829-1840 ◽  
Author(s):  
Thomas Wild ◽  
Marie Sofie Yoo Larsen ◽  
Takeo Narita ◽  
Julie Schou ◽  
Jakob Nilsson ◽  
...  
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells

scholarly journals Plk1 and Mps1 Cooperatively Regulate the Spindle Assembly Checkpoint in Human Cells

Cell Reports ◽  
2015 ◽  
Vol 12 (1) ◽  
pp. 66-78 ◽  
Author(s):  
Conrad von Schubert ◽  
Fabien Cubizolles ◽  
Jasmine M. Bracher ◽  
Tale Sliedrecht ◽  
Geert J.P.L. Kops ◽  
...  
Keyword(s):  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells

scholarly journals Functional and Structural Characterization of Bub3·BubR1 Interactions Required for Spindle Assembly Checkpoint Signaling in Human Cells

2016 ◽  
Vol 291 (21) ◽  
pp. 11252-11267 ◽  
Author(s):  
Florian Prinz ◽  
Vera Puetter ◽  
Simon J. Holton ◽  
Dorothee Andres ◽  
Christian M. Stegmann ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells ◽  
Checkpoint Signaling

scholarly journals The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes

2008 ◽  
Vol 183 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Christopher B. O'Connell ◽  
Jadranka Lončarek ◽  
Polla Hergert ◽  
Antonis Kourtidis ◽  
Douglas S. Conklin ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Human Cells

The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension.

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