scholarly journals Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling

Cell Reports ◽  
2018 ◽  
Vol 25 (3) ◽  
pp. 598-610.e5 ◽  
Author(s):  
Chengkang Zhang ◽  
Hyung Joo Lee ◽  
Anura Shrivastava ◽  
Ruipeng Wang ◽  
Travis J. McQuiston ◽  
...  
Keyword(s):  
Stem Cells ◽  
Myosin Ii ◽  
Epithelial Stem Cells ◽  
Pharmacological Inhibition ◽  
In Vitro Expansion

Effects Of Hypoxia in Long-Term In Vitro Expansion of Human Bone Marrow Derived Mesenchymal Stem Cells

2017 ◽  
Vol 118 (10) ◽  
pp. 3072-3079 ◽  
Author(s):  
Annelise Pezzi ◽  
Bruna Amorin ◽  
Álvaro Laureano ◽  
Vanessa Valim ◽  
Alice Dahmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vitro Expansion

scholarly journals Long-term In vitro Expansion Alters the Biology of Adult Mesenchymal Stem Cells

2008 ◽  
Vol 68 (11) ◽  
pp. 4229-4238 ◽  
Author(s):  
Reza Izadpanah ◽  
Deepak Kaushal ◽  
Christopher Kriedt ◽  
Fern Tsien ◽  
Bindiya Patel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adult Mesenchymal Stem Cells ◽  
In Vitro Expansion

scholarly journals Partial Maintenance and Long-Term Expansion of Murine Skin Epithelial Stem Cells by Wnt-3a In Vitro

2015 ◽  
Vol 135 (6) ◽  
pp. 1598-1608 ◽  
Author(s):  
Yukiteru Ouji ◽  
Shigeaki Ishizaka ◽  
Fukumi Nakamura-Uchiyama ◽  
Daisuke Okuzaki ◽  
Masahide Yoshikawa
Keyword(s):  
Stem Cells ◽  
Epithelial Stem Cells

scholarly journals In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection

2015 ◽  
Vol 148 (1) ◽  
pp. 126-136.e6 ◽  
Author(s):  
Sina Bartfeld ◽  
Tülay Bayram ◽  
Marc van de Wetering ◽  
Meritxell Huch ◽  
Harry Begthel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bacterial Infection ◽  
Epithelial Stem Cells ◽  
In Vitro Expansion

scholarly journals Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Makoto Takeo ◽  
Kyosuke Asakawa ◽  
Koh-ei Toyoshima ◽  
Miho Ogawa ◽  
JingJing Tong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicles ◽  
Regenerative Therapy ◽  
Functional Heterogeneity ◽  
Epithelial Stem Cells ◽  
Hair Regeneration ◽  
Bulge Region

AbstractIn mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin β5 (Itgβ5)-triple-positive (CD34+/CD49f+/Itgβ5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy.

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