scholarly journals STAT3 Regulates Self-Renewal of Adult Muscle Satellite Cells during Injury-Induced Muscle Regeneration

Cell Reports ◽  
2016 ◽  
Vol 16 (8) ◽  
pp. 2102-2115 ◽  
Author(s):  
Han Zhu ◽  
Fang Xiao ◽  
Gang Wang ◽  
Xiuqing Wei ◽  
Lei Jiang ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Muscle Regeneration ◽  
Self Renewal ◽  
Muscle Satellite Cells ◽  
Adult Muscle

scholarly journals MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression

2018 ◽  
Vol 46 (6) ◽  
pp. 2271-2283 ◽  
Author(s):  
Lianjie Hou ◽  
Jian Xu ◽  
Yiren Jiao ◽  
Huaqin Li ◽  
Zhicheng Pan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Western Blot ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Target Gene ◽  
Muscle Development ◽  
Muscle Satellite Cells ◽  
Qrt Pcr

Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to the skeletal muscle are common. Lifelong maintenance of skeletal muscle function largely depends on preserving the regenerative capacity of muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play an important role in muscle regeneration after injury. Therefore, understanding of the mechanisms associated with muscle development during muscle regeneration is essential for devising the alternative treatments for muscle injury in the future. Methods: Edu staining, qRT-PCR and western blot were used to evaluate the miR-27b effects on pig muscle satellite cells (PSCs) proliferation and differentiation in vitro. Then, we used bioinformatics analysis and dual-luciferase reporter assay to predict and confirm the miR-27b target gene. Finally, we elucidate the target gene function on muscle development in vitro and in vivo through Edu staining, qRT-PCR, western blot, H&E staining and morphological observation. Result: miR-27b inhibits PSCs proliferation and promotes PSCs differentiation. And the miR-27b target gene, MDFI, promotes PSCs proliferation and inhibits PSCs differentiation in vitro. Furthermore, interfering MDFI expression promotes mice muscle regeneration after injury. Conclusion: our results conclude that miR-27b promotes PSCs myogenesis by targeting MDFI. These results expand our understanding of muscle development mechanism in which miRNAs and genes work collaboratively in regulating skeletal muscle development. Furthermore, this finding has implications for obtaining the alternative treatments for patients with the muscle injury.

scholarly journals Muscle satellite cells are functionally impaired in myasthenia gravis: consequences on muscle regeneration

2017 ◽  
Vol 134 (6) ◽  
pp. 869-888 ◽  
Author(s):  
Mohamed Attia ◽  
Marie Maurer ◽  
Marieke Robinet ◽  
Fabien Le Grand ◽  
Elie Fadel ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Muscle Satellite Cells ◽  
Functionally Impaired

Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells

2006 ◽  
Vol 8 (7) ◽  
pp. 677-682 ◽  
Author(s):  
Vasily Shinin ◽  
Barbara Gayraud-Morel ◽  
Danielle Gomès ◽  
Shahragim Tajbakhsh
Keyword(s):  
Satellite Cells ◽  
Asymmetric Division ◽  
Muscle Satellite Cells ◽  
Adult Muscle ◽  
Dna Strands ◽  
Template Dna

Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle

Development ◽  
2012 ◽  
Vol 139 (7) ◽  
pp. e707-e707
Author(s):  
Y. Ono ◽  
S. Masuda ◽  
H.-s. Nam ◽  
R. Benezra ◽  
Y. Miyagoe-Suzuki ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Self Renewal ◽  
Adult Muscle

scholarly journals In Vivo Study of Key Transcription Factors in Muscle Satellite Cells by CRISPR/Cas9/AAV9-sgRNA Mediated Genome Editing

2019 ◽  
Author(s):  
Liangqiang He ◽  
Yingzhe Ding ◽  
Yu Zhao ◽  
Karl K. So ◽  
Xianlu L. Peng ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Genome Editing ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Molecular Mechanisms ◽  
Early Stage ◽  
Muscle Stem Cells ◽  
Muscle Satellite Cells ◽  
Regulatory Functions

ABSTRACTSkeletal muscle satellite cells (SCs) are adult muscle stem cells responsible for injury induced muscle regeneration. Despite advances in the knowledge of molecular mechanisms regulating SC lineage progression, our understanding of key transcription factors (TFs) and their regulatory functions in SCs in particularly the quiescent and early activation stages remains incomplete due to the lack of efficient method to screen and investigate the stage-specific key TFs. In this study, we succeeded in defining a distinct list of key TFs in early stages of SC fate transition using the paradigm of super enhancers (SEs). Particularly, leveraging the Cre-dependent Cas9 knockin mice and AAV9 mediated sgRNAs delivery, we generated a facile muscle specific genome editing system which allows gene depletion in SCs in vivo. Using MyoD locus as a proof of concept, we demonstrated that this CRISPR/Cas9/AAV9-sgRNA system can efficiently introduce mutagenesis at target locus and recapture the phenotypes reported in knockout mice. Further application of the system on key TFs, Myc, Bcl6 and Pknox2, revealed their distinct functions in the early stage of SC activation and damage induced muscle regeneration. Altogether our findings have proven the CRISPR/Cas9/AAV9-sgRNA system as a robust way for in vivo genome editing and elucidation of key factors governing SC activities.

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