scholarly journals Targeted mRNA Decay by RNA Binding Protein AUF1 Regulates Adult Muscle Stem Cell Fate, Promoting Skeletal Muscle Integrity

Cell Reports ◽  
2016 ◽  
Vol 16 (5) ◽  
pp. 1379-1390 ◽  
Author(s):  
Devon M. Chenette ◽  
Adam B. Cadwallader ◽  
Tiffany L. Antwine ◽  
Lauren C. Larkin ◽  
Jinhua Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Cell Fate ◽  
Mrna Decay ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Muscle Stem Cell ◽  
Stem Cell Fate ◽  
Adult Muscle

scholarly journals Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs

2019 ◽  
Vol 48 (4) ◽  
pp. 1843-1871 ◽  
Author(s):  
René M Arvola ◽  
Chung-Te Chang ◽  
Joseph P Buytendorp ◽  
Yevgen Levdansky ◽  
Eugene Valkov ◽  
...  
Keyword(s):  
Stem Cell ◽  
Catalytic Activity ◽  
Protein Expression ◽  
Cell Fate ◽  
Rna Binding ◽  
Rna Binding Protein ◽  
Stem Cell Fate ◽  
Target Mrnas ◽  
N Terminus ◽  
Decapping Enzyme

Abstract Pumilio is an RNA-binding protein that represses a network of mRNAs to control embryogenesis, stem cell fate, fertility and neurological functions in Drosophila. We sought to identify the mechanism of Pumilio-mediated repression and find that it accelerates degradation of target mRNAs, mediated by three N-terminal Repression Domains (RDs), which are unique to Pumilio orthologs. We show that the repressive activities of the Pumilio RDs depend on specific subunits of the Ccr4–Not (CNOT) deadenylase complex. Depletion of Pop2, Not1, Not2, or Not3 subunits alleviates Pumilio RD-mediated repression of protein expression and mRNA decay, whereas depletion of other CNOT components had little or no effect. Moreover, the catalytic activity of Pop2 deadenylase is important for Pumilio RD activity. Further, we show that the Pumilio RDs directly bind to the CNOT complex. We also report that the decapping enzyme, Dcp2, participates in repression by the N-terminus of Pumilio. These results support a model wherein Pumilio utilizes CNOT deadenylase and decapping complexes to accelerate destruction of target mRNAs. Because the N-terminal RDs are conserved in mammalian Pumilio orthologs, the results of this work broadly enhance our understanding of Pumilio function and roles in diseases including cancer, neurodegeneration and epilepsy.

scholarly journals Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs

2019 ◽  
Author(s):  
René M. Arvola ◽  
Chung-Te Chang ◽  
Joseph P. Buytendorp ◽  
Yevgen Levdansky ◽  
Eugene Valkov ◽  
...  
Keyword(s):  
Stem Cell ◽  
Catalytic Activity ◽  
Protein Expression ◽  
Cell Fate ◽  
Rna Binding ◽  
Rna Binding Protein ◽  
Stem Cell Fate ◽  
Target Mrnas ◽  
N Terminus ◽  
Decapping Enzyme

ABSTRACTPumilio is an RNA-binding protein that represses a network of mRNAs to control embryogenesis, stem cell fate, fertility, and neurological functions in Drosophila. We sought to identify the mechanism of Pumilio-mediated repression and find that it accelerates degradation of target mRNAs, mediated by three N-terminal Repression Domains (RDs), which are unique to Pumilio orthologs. We show that the repressive activities of the Pumilio RDs depend on specific subunits of the Ccr4-Not (CNOT) deadenylase complex. Depletion of Pop2, Not1, Not2, or Not3 subunits alleviates Pumilio RD-mediated repression of protein expression and mRNA decay, whereas depletion of other CNOT components had little or no effect. Moreover, the catalytic activity of Pop2 deadenylase is important for Pumilio RD activity. Further, we show that the Pumilio RDs directly bind to the CNOT complex. We also report that the decapping enzyme, Dcp2, participates in repression by the N-terminus of Pumilio. These results support a model wherein Pumilio utilizes CNOT deadenylase and decapping complexes to accelerate destruction of target mRNAs. Because the N-terminal RDs are conserved in mammalian Pumilio orthologs, the results of this work broadly enhance our understanding of Pumilio function and roles in diseases including cancer, neurodegeneration, and epilepsy.

scholarly journals p38α MAPK Regulates Adult Muscle Stem Cell Fate by Restricting Progenitor Proliferation During Postnatal Growth and Repair

Stem Cells ◽  
2013 ◽  
Vol 31 (8) ◽  
pp. 1597-1610 ◽  
Author(s):  
Patrick Brien ◽  
Dhamayanthi Pugazhendhi ◽  
Samuel Woodhouse ◽  
David Oxley ◽  
Jennifer M. Pell
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Postnatal Growth ◽  
Muscle Stem Cell ◽  
Stem Cell Fate ◽  
Adult Muscle ◽  
P38α Mapk

scholarly journals The RNA‐binding protein Imp2 regulates oxidative phosporylation that is key to glioblastoma cancer stem cell maintenance

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Michalina Janiszewska ◽  
Mario-Luca Suva ◽  
Riekelt H. Houtkooper ◽  
Virginie Clement-Schatlo ◽  
Ivan Stamenkovic
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Stem Cell Maintenance ◽  
Cell Maintenance

scholarly journals RNA binding protein RBM3 promotes a cancer stem cell phenotype with multidrug resistance

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Anand Venugopal ◽  
Deep Kwatra ◽  
Shane Stecklein ◽  
Satish Ramalingam ◽  
Dharmalingam Subramaniam ◽  
...  
Keyword(s):  
Stem Cell ◽  
Multidrug Resistance ◽  
Cancer Stem Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Cell Phenotype ◽  
Cancer Stem Cell Phenotype ◽  
Stem Cell Phenotype
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