scholarly journals Immunogenic Stimulus for Germline Precursors of Antibodies that Engage the Influenza Hemagglutinin Receptor-Binding Site

Cell Reports ◽  
2015 ◽  
Vol 13 (12) ◽  
pp. 2842-2850 ◽  
Author(s):  
Aaron G. Schmidt ◽  
Khoi T. Do ◽  
Kevin R. McCarthy ◽  
Thomas B. Kepler ◽  
Hua-Xin Liao ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
Receptor Binding Site ◽  
Influenza Hemagglutinin

scholarly journals Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin

2017 ◽  
Vol 21 (6) ◽  
pp. 742-753.e8 ◽  
Author(s):  
Nicholas C. Wu ◽  
Jia Xie ◽  
Tianqing Zheng ◽  
Corwin M. Nycholat ◽  
Geramie Grande ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
Receptor Binding Site ◽  
Influenza Hemagglutinin

scholarly journals Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin

2017 ◽  
Vol 22 (2) ◽  
pp. 247-248 ◽  
Author(s):  
Nicholas C. Wu ◽  
Jia Xie ◽  
Tianqing Zheng ◽  
Corwin M. Nycholat ◽  
Geramie Grande ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
Receptor Binding Site ◽  
Influenza Hemagglutinin

scholarly journals A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin

2013 ◽  
Vol 20 (3) ◽  
pp. 363-370 ◽  
Author(s):  
Rui Xu ◽  
Jens C Krause ◽  
Ryan McBride ◽  
James C Paulson ◽  
James E Crowe ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
Receptor Binding Site ◽  
Antibody Recognition ◽  
Influenza Hemagglutinin

scholarly journals A mutation in the receptor binding site enhances infectivity of 2009 H1N1 influenza hemagglutinin pseudotypes without changing antigenicity

Virology ◽  
2010 ◽  
Vol 407 (2) ◽  
pp. 374-380 ◽  
Author(s):  
Wei Wang ◽  
Juan A. Castelán-Vega ◽  
Alicia Jiménez-Alberto ◽  
Russell Vassell ◽  
Zhiping Ye ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
H1n1 Influenza ◽  
Receptor Binding Site ◽  
Influenza Hemagglutinin ◽  
2009 H1n1

scholarly journals A complex epistatic network limits the mutational reversibility in the influenza hemagglutinin receptor-binding site

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Nicholas C. Wu ◽  
Andrew J. Thompson ◽  
Jia Xie ◽  
Chih-Wei Lin ◽  
Corwin M. Nycholat ◽  
...  
Keyword(s):  
Binding Site ◽  
Receptor Binding ◽  
Receptor Binding Site ◽  
Influenza Hemagglutinin

scholarly journals Potent sialic acid inhibitors that target influenza A virus hemagglutinin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yu-Jen Chang ◽  
Cheng-Yun Yeh ◽  
Ju-Chien Cheng ◽  
Yu-Qi Huang ◽  
Kai-Cheng Hsu ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Antiviral Activity ◽  
Binding Site ◽  
Influenza A Virus ◽  
Receptor Binding ◽  
Influenza A ◽  
The United States ◽  
Ligand Complex ◽  
Receptor Binding Site ◽  
Computational Strategy

AbstractEradicating influenza A virus (IAV) is difficult, due to its genetic drift and reassortment ability. As the infectious cycle is initiated by the influenza glycoprotein, hemagglutinin (HA), which mediates the binding of virions to terminal sialic acids moieties, HA is a tempting target of anti-influenza inhibitors. However, the complexity of the HA structure has prevented delineation of the structural characterization of the HA protein–ligand complex. Our computational strategy efficiently analyzed > 200,000 records of compounds held in the United States National Cancer Institute (NCI) database and identified potential HA inhibitors, by modeling the sialic acid (SA) receptor binding site (RBS) for the HA structure. Our modeling revealed that compound NSC85561 showed significant antiviral activity against the IAV H1N1 strain with EC50 values ranging from 2.31 to 2.53 µM and negligible cytotoxicity (CC50 > 700 µM). Using the NSC85561 compound as the template to generate 12 derivatives, robust bioassay results revealed the strongest antiviral efficacies with NSC47715 and NSC7223. Virtual screening clearly identified three SA receptor binding site inhibitors that were successfully validated in experimental data. Thus, our computational strategy has identified SA receptor binding site inhibitors against HA that show IAV-associated antiviral activity.

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