scholarly journals Both Chromosome Decondensation and Condensation Are Dependent on DNA Replication in C. elegans Embryos

Cell Reports ◽  
2015 ◽  
Vol 12 (3) ◽  
pp. 405-417 ◽  
Author(s):  
Remi Sonneville ◽  
Gillian Craig ◽  
Karim Labib ◽  
Anton Gartner ◽  
J. Julian Blow
Keyword(s):  
Dna Replication ◽  
C Elegans ◽  
Chromosome Decondensation

scholarly journals Loss of histone H3.3 results in DNA replication defects and altered origin dynamics in C. elegans

2020 ◽  
Vol 30 (12) ◽  
pp. 1740-1751
Author(s):  
Maude Strobino ◽  
Joanna M. Wenda ◽  
Laura Padayachy ◽  
Florian A. Steiner
Keyword(s):  
Dna Replication ◽  
C Elegans ◽  
Histone H3.3

scholarly journals Cell-Type Specific Responses to DNA Replication Stress in Early C. elegans Embryos

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164601 ◽  
Author(s):  
Holly Stevens ◽  
Ashley B. Williams ◽  
W. Matthew Michael
Keyword(s):  
Dna Replication ◽  
Replication Stress ◽  
Cell Type ◽  
C Elegans ◽  
Dna Replication Stress ◽  
Cell Type Specific

scholarly journals Functional redundancy of two C. elegans homologs of the histone chaperone Asf1 in germline DNA replication

2009 ◽  
Vol 329 (1) ◽  
pp. 64-79 ◽  
Author(s):  
Iwen F. Grigsby ◽  
Eric M. Rutledge ◽  
Christine A. Morton ◽  
Fern P. Finger
Keyword(s):  
Dna Replication ◽  
Functional Redundancy ◽  
Histone Chaperone ◽  
C Elegans

scholarly journals Staphylococcus aureus Virulence Factors Identified by Using a High-Throughput Caenorhabditis elegans-Killing Model

2005 ◽  
Vol 73 (2) ◽  
pp. 872-877 ◽  
Author(s):  
Jakob Begun ◽  
Costi D. Sifri ◽  
Samuel Goldman ◽  
Stephen B. Calderwood ◽  
Frederick M. Ausubel
Keyword(s):  
Staphylococcus Aureus ◽  
Caenorhabditis Elegans ◽  
Dna Replication ◽  
Virulence Factors ◽  
High Throughput ◽  
Human Pathogen ◽  
Renal Abscess ◽  
C Elegans ◽  
Transposon Insertion ◽  
Strain Nctc

ABSTRACT Staphylococcus aureus is an important human pathogen that is also able to kill the model nematode Caenorhabditis elegans. We constructed a 2,950-member Tn917 transposon insertion library in S. aureus strain NCTC 8325. Twenty-one of these insertions exhibited attenuated C. elegans killing, and of these, 12 contained insertions in different genes or chromosomal locations. Ten of these 12 insertions showed attenuated killing phenotypes when transduced into two different S. aureus strains, and 5 of the 10 mutants correspond to genes that have not been previously identified in signature-tagged mutagenesis studies. These latter five mutants were tested in a murine renal abscess model, and one mutant harboring an insertion in nagD exhibited attenuated virulence. Interestingly, Tn917 was shown to have a very strong bias for insertions near the terminus of DNA replication.

scholarly journals Spatiotemporal coupling and decoupling of gene transcription with DNA replication origins during embryogenesis in C. elegans

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Ehsan Pourkarimi ◽  
James M Bellush ◽  
Iestyn Whitehouse
Keyword(s):  
Dna Replication ◽  
Developmental Stages ◽  
Embryonic Cell ◽  
Genome Replication ◽  
Replication Origins ◽  
C Elegans ◽  
Cellular Changes ◽  
Zygotic Transcription ◽  
Close Proximity ◽  
Dna Replication Origins

The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans, we show that replication origins are marked with specific histone modifications that define gene enhancers. We demonstrate that the level of enhancer associated modifications scale with the efficiency at which the origin is utilized. By mapping replication origins at different developmental stages, we show that the positions and activity of origins is largely invariant through embryogenesis. Contrary to expectation, we find that replication origins are specified prior to the broad onset of zygotic transcription, yet when transcription initiates it does so in close proximity to the pre-defined replication origins. Transcription and DNA replication origins are correlated, but the association breaks down when embryonic cell division ceases. Collectively, our data indicate that replication origins are fundamental organizers and regulators of gene activity through embryonic development.

scholarly journals C. elegans MCM-4 is a general DNA replication and checkpoint component with an epidermis-specific requirement for growth and viability

2011 ◽  
Vol 350 (2) ◽  
pp. 358-369 ◽  
Author(s):  
Jerome Korzelius ◽  
Inge The ◽  
Suzan Ruijtenberg ◽  
Vincent Portegijs ◽  
Huihong Xu ◽  
...  
Keyword(s):  
Dna Replication ◽  
Specific Requirement ◽  
C Elegans
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