scholarly journals Systematic Perturbation of Cytoskeletal Function Reveals a Linear Scaling Relationship between Cell Geometry and Fitness

Cell Reports ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. 1528-1537 ◽  
Author(s):  
Russell D. Monds ◽  
Timothy K. Lee ◽  
Alexandre Colavin ◽  
Tristan Ursell ◽  
Selwyn Quan ◽  
...  
Keyword(s):  
Linear Scaling ◽  
Cell Geometry ◽  
Scaling Relationship ◽  
Cytoskeletal Function ◽  
Systematic Perturbation

scholarly journals Functional Exploration of Heterotrimeric Kinesin-II in IFT and Ciliary Length Control in Chlamydomonas

2020 ◽  
Author(s):  
Shufen Li ◽  
Wei Chen ◽  
Kirsty Y Wan ◽  
Hui Tao ◽  
Xin Liang ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Injection Rate ◽  
Linear Scaling ◽  
Motor Speed ◽  
Fold Reduction ◽  
Scaling Relationship ◽  
Non Linear ◽  
Assembly Activity

SUMMARYHeterotrimeric organization of kinesin-II is essential for its function in anterograde IFT in ciliogenesis. However, the molecular basis of forming this complex for its function is not well understood. In addition, the anterograde IFT velocity varies significantly in different organisms, but how motor speed affects ciliary length is not clear. We show that Chlamydomonas kinesin-II (CrKinesin-II) involves distinct mechanisms from mammals and C. elegans in its assembly to necessitate its function in IFT. Furthermore, chimeric CrKinesin-II with human kinesin-II motor domains functioned in vitro and in vivo, leading to a ~2.8-fold reduced anterograde IFT velocity and a similar fold reduction in IFT injection rate that supposedly correlates with ciliary assembly activity. However, the ciliary length was only mildly reduced (~15%). Modelling analyses suggest that such a non-linear scaling relationship between IFT velocity and ciliary length can be accounted for by limitation of the motors and/or its ciliary cargoes, e.g. tubulin.

Phase slip scaling relationship for the 59 K and 143 K charge-density-waves in NbSe3

1999 ◽  
Vol 09 (PR10) ◽  
pp. Pr10-129-Pr10-132 ◽  
Author(s):  
J. P. McCarten ◽  
T. C. Jones ◽  
X. Wu ◽  
J. H. Miller ◽  
I. Pirtle ◽  
...  
Keyword(s):  
Charge Density ◽  
Charge Density Waves ◽  
Phase Slip ◽  
Density Waves ◽  
Scaling Relationship

Novel Products in the CO2-Laser Induced Reaction of Trichloroethylene

1995 ◽  
Vol 60 (1) ◽  
pp. 104-114 ◽  
Author(s):  
Boyd L. Earl ◽  
Richard L. Titus
Keyword(s):  
Irradiation Time ◽  
Product Distribution ◽  
Incident Power ◽  
Cell Geometry ◽  
Reaction Conditions ◽  
Peak Areas ◽  
Induced Decomposition ◽  
Nmr Methods ◽  
Induced Reaction ◽  
Novel Products

Previous reports on the thermal or CO2-laser induced decomposition of trichloroethylene have identified only one condensible product, hexachlorobenzene (in addition to HCl and mono- and dichloroacetylene). We have found that trichloroethylene vapor exposed to cw irradiation on the P(24) line of the (001 - 100) band of the CO2 laser at incident power levels from 8 - 17 W produces numerous products, of which the 13 major ones have been identified using IR, GC/MS, GC/FTIR, and NMR methods. All of these products have 4, 6, or 8 carbons, are highly unsaturated, and are completely chlorinated or contain a single hydrogen. C4HCl5 and C6Cl6 isomers (three of each) account for S 55% to 85% of total products (based on peak areas in the total ion chromatograms in GC/MS runs), depending on reaction conditions. In addition to characterizing the products, we discuss the dependence of the product distribution on laser power, irradiation time, and cell geometry, and we outline a possible mechanism.

Sign in / Sign up

Export Citation Format

Share Document