scholarly journals DNA Copy-Number Control through Inhibition of Replication Fork Progression

Cell Reports ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. 841-849 ◽  
Author(s):  
Jared T. Nordman ◽  
Elena N. Kozhevnikova ◽  
C. Peter Verrijzer ◽  
Alexey V. Pindyurin ◽  
Evgeniya N. Andreyeva ◽  
...  
Keyword(s):  
Copy Number ◽  
Replication Fork ◽  
Dna Copy Number ◽  
Copy Number Control ◽  
Replication Fork Progression

scholarly journals Author response: Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

2018 ◽  
Author(s):  
Alexander Munden ◽  
Zhan Rong ◽  
Amanda Sun ◽  
Rama Gangula ◽  
Simon Mallal ◽  
...  
Keyword(s):  
Copy Number ◽  
Replication Fork ◽  
Author Response ◽  
Dna Copy Number ◽  
Replication Fork Progression

scholarly journals Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

2018 ◽  
Author(s):  
Alexander Munden ◽  
Zhan Rong ◽  
Rama Gangula ◽  
Simon Mallal ◽  
Jared T. Nordman
Keyword(s):  
Copy Number ◽  
Replication Fork ◽  
Genome Stability ◽  
Replication Timing ◽  
Physical Interaction ◽  
Dependent Manner ◽  
Replication Forks ◽  
Dna Copy Number ◽  
Replication Fork Progression ◽  
Maintain Genome Stability

ABSTRACTControl of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the genome to promote DNA underreplication. While dissecting the function of SUUR’s SNF2 domain, we identified a physical interaction between SUUR and Rif1. Rif1 has many roles in DNA metabolism and regulates the replication timing program. We demonstrate that repression of DNA replication is dependent on Rif1. Rif1 localizes to active replication forks in an SUUR-dependent manner and directly regulates replication fork progression. Importantly, SUUR associates with replication forks in the absence of Rif1, indicating that Rif1 acts downstream of SUUR to inhibit fork progression. Our findings uncover an unrecognized function of the Rif1 protein as a regulator of replication fork progression.

scholarly journals Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Alexander Munden ◽  
Zhan Rong ◽  
Amanda Sun ◽  
Rama Gangula ◽  
Simon Mallal ◽  
...  
Keyword(s):  
Copy Number ◽  
Replication Fork ◽  
Genome Stability ◽  
Replication Timing ◽  
Dependent Manner ◽  
Replication Forks ◽  
Dna Copy Number ◽  
Replication Fork Progression ◽  
Suur Protein ◽  
Maintain Genome Stability

Control of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the genome to promote DNA underreplication. While dissecting the function of SUUR’s SNF2 domain, we identified an interaction between SUUR and Rif1. Rif1 has many roles in DNA metabolism and regulates the replication timing program. We demonstrate that repression of DNA replication is dependent on Rif1. Rif1 localizes to active replication forks in a partially SUUR-dependent manner and directly regulates replication fork progression. Importantly, SUUR associates with replication forks in the absence of Rif1, indicating that Rif1 acts downstream of SUUR to inhibit fork progression. Our findings uncover an unrecognized function of the Rif1 protein as a regulator of replication fork progression.

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