scholarly journals In Vitro-Reconstituted Nucleoids Can Block Mitochondrial DNA Replication and Transcription

Cell Reports ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Géraldine Farge ◽  
Majda Mehmedovic ◽  
Marian Baclayon ◽  
Siet M.J.L. van den Wildenberg ◽  
Wouter H. Roos ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Mitochondrial Dna Replication

scholarly journals Genetic and molecular analysis of the SOE1 gene: a tRNA(3Glu) missense suppressor of yeast cdc8 mutations.

Genetics ◽  
1990 ◽  
Vol 124 (3) ◽  
pp. 523-531 ◽  
Author(s):  
J Y Su ◽  
L Belmont ◽  
R A Sclafani
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Kinase Activity ◽  
Gene Dosage ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Chromosome X ◽  
Mitochondrial Functions ◽  
Mitochondrial Dna Replication

Abstract The CDC8 gene of Saccharomyces cerevisiae encodes deoxythymidylate (dTMP) kinase and is required for nuclear and mitochondrial DNA replication in both the mitotic and meiotic cell cycles. All cdc8 temperature-sensitive mutants are partially defective in meiotic and mitochondrial functions at the permissive temperature. In a study of revertants of temperature-sensitive cdc8 mutants, the SOE201 and SOE1 mutants were isolated. The SOE201 mutant is a disome of chromosome X to which the cdc8 gene maps. Using the chromosome X aneuploids to vary cdc8 gene dosage, we demonstrate that different levels of dTMP kinase activity are required for mitotic, meiotic or mitochondrial DNA replication. The SOE1 mutant contains a dominant suppressor that suppresses five different cdc8 alleles but does not suppress a complete cdc8 deletion. The SOE1 gene is located less than 1.5 cM from the CYH2 gene on chromosome VII and is adjacent to the TSM437-CYH2 region, with the gene order being SOE1-TSM437-CYH2. SOE1 is an inefficient suppressor that can neither suppress the cdc8 hypomorphic phenotype nor restore dTMP kinase activity in vitro. SOE1 is a single C to T mutation in the anticodon of a tRNA(3Glu) gene and thereby, produces a missense suppressor tRNA capable of recognizing AAA lysine codons. We propose that the resultant lysine to glutamate change stabilizes thermo-labile dTMP kinase molecules in the cell.

Selective inhibition of mutant human mitochondrial DNA replication in vitro by peptide nucleic acids

1997 ◽  
Vol 15 (2) ◽  
pp. 212-215 ◽  
Author(s):  
Robert W. Taylor ◽  
Patrick F. Chinnery ◽  
Douglass M. Turnbull ◽  
Robert N. Lightowlers
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Selective Inhibition ◽  
Human Mitochondrial Dna ◽  
Mitochondrial Dna Replication
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