scholarly journals Slit/Robo Signaling Regulates Cell Fate Decisions in the Intestinal Stem Cell Lineage of Drosophila

Cell Reports ◽  
2014 ◽  
Vol 7 (6) ◽  
pp. 1867-1875 ◽  
Author(s):  
Benoît Biteau ◽  
Heinrich Jasper
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Cell Lineage ◽  
Intestinal Stem Cell ◽  
Cell Fate Decisions ◽  
Stem Cell Lineage

scholarly journals Intracellular pH dynamics regulates intestinal stem cell fate

2021 ◽  
Author(s):  
Diane L. Barber ◽  
Yi Liu ◽  
Efren Reyes ◽  
David Castillo-Azofeifa ◽  
Ophir D Klein ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Intracellular Ph ◽  
Paneth Cell ◽  
Cell Lineage ◽  
Adult Stem Cell ◽  
Intestinal Stem Cell ◽  
Cell Fate Specification ◽  
Stem Cell Fate ◽  
Stem Cell Lineage

Emerging evidence is revealing critical roles of intracellular pH (pHi) in development, but it remains unclear whether pHi regulates stem cell fate specification. We find that pHi dynamics is a key regulator of cell fate in the mouse intestinal stem cell lineage. We identify a pHi gradient along the intestinal crypt axis and find that dissipating this gradient inhibits crypt budding due to loss Paneth cell differentiation. Mechanistically, decreasing pHi biases intestinal stem cell fate toward the absorptive and away from the secretory lineage, by regulating the activity of the lineage transcription factor Atoh1. Our findings reveal a previously unrecognized role for pHi dynamics in the specification of cell fate within an adult stem cell lineage.

scholarly journals Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates

2021 ◽  
Vol 23 (1) ◽  
pp. 23-31
Author(s):  
Anika Böttcher ◽  
Maren Büttner ◽  
Sophie Tritschler ◽  
Michael Sterr ◽  
Alexandra Aliluev ◽  
...  
Keyword(s):  
Stem Cell ◽  
Paneth Cell ◽  
Cell Lineage ◽  
Intestinal Stem Cell ◽  
Cell Fates ◽  
Stem Cell Lineage ◽  
Lineage Priming ◽  
Canonical Wnt

scholarly journals Biomimetic three-dimensional microenvironment for controlling stem cell fate

2011 ◽  
Vol 1 (5) ◽  
pp. 792-803 ◽  
Author(s):  
Hu Zhang ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
Kuo-Kang Liu
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Cell Lineage ◽  
Cell Interaction ◽  
Stem Cell Fate ◽  
Cell Microenvironment ◽  
Stem Cell Lineage ◽  
Matrix Cell ◽  
The Individual

Stem cell therapy is an emerging technique which is being translated into treatment of degenerated tissues. However, the success of translation relies on the stem cell lineage commitment in the degenerated regions of interest. This commitment is precisely controlled by the stem cell microenvironment. Engineering a biomimetic three-dimensional microenvironment enables a thorough understanding of the mechanisms of governing stem cell fate. We review the individual microenvironment components, including soluble factors, extracellular matrix, cell–cell interaction and mechanical stimulation. The perspectives in creating the biomimetic microenvironments are discussed with emerging techniques.

scholarly journals Bi-directional cell-pericellular matrix interactions direct stem cell fate

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Silvia A. Ferreira ◽  
Meghna S. Motwani ◽  
Peter A. Faull ◽  
Alexis J. Seymour ◽  
Tracy T. L. Yu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Cell Lineage ◽  
Local Environment ◽  
Pericellular Matrix ◽  
Lineage Specification ◽  
Reciprocal Interactions ◽  
Stem Cell Lineage ◽  
Matrix Interactions ◽  
Insight Into

Abstract Modifiable hydrogels have revealed tremendous insight into how physical characteristics of cells’ 3D environment drive stem cell lineage specification. However, in native tissues, cells do not passively receive signals from their niche. Instead they actively probe and modify their pericellular space to suit their needs, yet the dynamics of cells’ reciprocal interactions with their pericellular environment when encapsulated within hydrogels remains relatively unexplored. Here, we show that human bone marrow stromal cells (hMSC) encapsulated within hyaluronic acid-based hydrogels modify their surroundings by synthesizing, secreting and arranging proteins pericellularly or by degrading the hydrogel. hMSC’s interactions with this local environment have a role in regulating hMSC fate, with a secreted proteinaceous pericellular matrix associated with adipogenesis, and degradation with osteogenesis. Our observations suggest that hMSC participate in a bi-directional interplay between the properties of their 3D milieu and their own secreted pericellular matrix, and that this combination of interactions drives fate.

Integrin αEβ7+ T cells direct intestinal stem cell fate decisions via adhesion signaling

Cell Research ◽  
2021 ◽  
Author(s):  
Shiyang Chen ◽  
Yajuan Zheng ◽  
Xiaojuan Ran ◽  
Hui Du ◽  
Hua Feng ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Cell Fate ◽  
Intestinal Stem Cell ◽  
Stem Cell Fate ◽  
Cell Fate Decisions

scholarly journals Telomere dysfunction cooperates with epigenetic alterations to impair murine embryonic stem cell fate commitment

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Mélanie Criqui ◽  
Aditi Qamra ◽  
Tsz Wai Chu ◽  
Monika Sharma ◽  
Julissa Tsao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Embryonic Stem ◽  
Cell Lineage ◽  
Chromatin Accessibility ◽  
Telomere Dysfunction ◽  
Epigenetic Alterations ◽  
Dna Hypomethylation ◽  
Murine Embryonic Stem Cell ◽  
Stem Cell Lineage

The precise relationship between epigenetic alterations and telomere dysfunction is still an extant question. Previously, we showed that eroded telomeres lead to differentiation instability in murine embryonic stem cells (mESCs) via DNA hypomethylation at pluripotency-factor promoters. Here, we uncovered that telomerase reverse transcriptase null (Tert-/-) mESCs exhibit genome-wide alterations in chromatin accessibility and gene expression during differentiation. These changes were accompanied by an increase of H3K27me3 globally, an altered chromatin landscape at the Pou5f1/Oct4 promoter, and a refractory response to differentiation cues. Inhibition of the Polycomb Repressive Complex 2 (PRC2), an H3K27 tri-methyltransferase, exacerbated the impairment in differentiation and pluripotency gene repression in Tert-/- mESCs but not wild-type mESCs, whereas inhibition of H3K27me3 demethylation led to a partial rescue of the Tert-/- phenotype. These data reveal a new interdependent relationship between H3K27me3 and telomere integrity in stem cell lineage commitment that may have implications in aging and cancer.

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