scholarly journals Lineage Tracing and Cell Ablation Identify a Post-Aire-Expressing Thymic Epithelial Cell Population

Cell Reports ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 166-179 ◽  
Author(s):  
Todd C. Metzger ◽  
Imran S. Khan ◽  
James M. Gardner ◽  
Maria L. Mouchess ◽  
Kellsey P. Johannes ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Population ◽  
Lineage Tracing ◽  
Thymic Epithelial Cell ◽  
Cell Ablation ◽  
Epithelial Cell Population

Manual Exfoliation Plus Immunomagnetic Bead Separation as an Initial Step Toward Translational Research

2006 ◽  
Vol 130 (1) ◽  
pp. 74-79
Author(s):  
Wilfrido D. Mojica ◽  
Ayesha Arshad ◽  
Sanjay Sharma ◽  
Stephen P. Brooks
Keyword(s):  
Epithelial Cell ◽  
Nucleic Acids ◽  
Cell Population ◽  
High Throughput ◽  
Human Disease ◽  
Model Systems ◽  
High Quality ◽  
High Throughput Analysis ◽  
Immunomagnetic Bead ◽  
Epithelial Cell Population

Abstract Context.—The development of biotechnologic platforms capable of high throughput analysis has ushered in a promising new era of translational medicine. However, most studies to date are based on in vitro cell lines or substitute models for human disease. Although these model systems have proven insightful, it is readily becoming apparent that human clinical tissue must be studied in order to fully understand all the nuances of human disease. Studies that are based on human tissue, however, are limited by qualitative and quantitative issues, factors often precluding their use in high throughput studies. Objective.—To develop a simple and rapid tissue procurement protocol for use in obtaining a homogeneous epithelial cell population from clinical tissue and the recovery of nucleic acids and proteins of high quality and quantity. Also, to determine if the technique preserves tissue, thereby allowing morphologic correlation with molecular findings. Design.—Performance of manual exfoliation to procure cells from clinical resection specimens and use of immunomagnetic beads embedded with the antibody ber-Ep4 for the positive enrichment of a homogeneous epithelial cell population. Nucleic acids and proteins are then separated using a phenol plus guanidine thiocyante solution. Nucleic acids and proteins are quantitated and qualitatively analyzed using standard laboratory techniques. Results.—Nucleic acids and proteins of high quality and quantity were recovered following manual exfoliation and immunomagnetic bead separation. Tissue architecture was not destroyed, thus permitting histologic and molecular correlation. Conclusions.—A simple and reproducible protocol is presented that may enable the molecular profiling of clinically resected tissue. Although the technique is currently limited to certain tissue and tumor types, further research will broaden its overall application.

Clonogenic assay allows for selection of a primitive mammary epithelial cell population in bovine

2015 ◽  
Vol 338 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Eugenio Martignani ◽  
Diego Cravero ◽  
Silvia Miretti ◽  
Paolo Accornero ◽  
Mario Baratta
Keyword(s):  
Epithelial Cell ◽  
Cell Population ◽  
Mammary Epithelial Cell ◽  
Clonogenic Assay ◽  
Mammary Epithelial ◽  
Epithelial Cell Population ◽  
Selection Of

Whole Lung Single Cell RNAseq Reveals a Unique IPF-Specific Epithelial Cell Population

2019 ◽  
Author(s):  
J.C. Schupp ◽  
T. Adams ◽  
N. Neumark ◽  
S. Poli De Frias ◽  
F. Ahangari ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Single Cell ◽  
Cell Population ◽  
Epithelial Cell Population
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