scholarly journals Stage-Specific Regulation of Reprogramming to Induced Pluripotent Stem Cells by Wnt Signaling and T Cell Factor Proteins

Cell Reports ◽  
2013 ◽  
Vol 3 (6) ◽  
pp. 2113-2126 ◽  
Author(s):  
Ritchie Ho ◽  
Bernadett Papp ◽  
Jackson A. Hoffman ◽  
Bradley J. Merrill ◽  
Kathrin Plath
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Wnt Signaling ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
T Cell Factor ◽  
Specific Regulation ◽  
Induced Pluripotent

scholarly journals Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Young Sun Hwang ◽  
Shinnosuke Suzuki ◽  
Yasunari Seita ◽  
Jumpei Ito ◽  
Yuka Sakata ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cell ◽  
Postnatal Period ◽  
Germline Development ◽  
Specific Regulation ◽  
Induced Pluripotent

Abstract Establishment of spermatogonia throughout the fetal and postnatal period is essential for production of spermatozoa and male fertility. Here, we establish a protocol for in vitro reconstitution of human prospermatogonial specification whereby human primordial germ cell (PGC)-like cells differentiated from human induced pluripotent stem cells are further induced into M-prospermatogonia-like cells and T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single cell RNA-sequencing is used to delineate the lineage trajectory leading to T1LCs, which closely resemble human T1-prospermatogonia in vivo and exhibit gene expression related to spermatogenesis and diminished proliferation, a hallmark of quiescent T1 prospermatogonia. Notably, this system enables us to visualize the dynamic and stage-specific regulation of transposable elements during human prospermatogonial specification. Together, our findings pave the way for understanding and reconstructing human male germline development in vitro.

scholarly journals In Vitro T-Cell Generation From Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination

Stem Cells ◽  
2015 ◽  
Vol 33 (11) ◽  
pp. 3174-3180 ◽  
Author(s):  
Michelle J. Smith ◽  
Beau R. Webber ◽  
Mahmood Mohtashami ◽  
Heather E. Stefanski ◽  
Juan Carlos Zún˜iga-Pflücker ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Generation ◽  
Induced Pluripotent

scholarly journals Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

2020 ◽  
Author(s):  
Young Sun Hwang ◽  
Shinnosuke Suzuki ◽  
Yasunari Seita ◽  
Jumpei Ito ◽  
Yuka Sakata ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cell ◽  
Postnatal Period ◽  
Germline Development ◽  
Specific Regulation ◽  
Induced Pluripotent

ABSTRACTEstablishment of spermatogonia throughout the fetal and postnatal period is essential for production of spermatozoa and male fertility. Here, we established a protocol for in vitro reconstitution of human prospermatogonial specification whereby human primordial germ cell (PGC)-like cells (hPGCLCs) differentiated from human induced pluripotent stem cells were further induced into M-prospermatogonia-like cells (MLCs) and T1 prospermatogonia-like cells (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single cell RNA-sequencing was used to delineate the lineage trajectory leading to T1LCs, which closely resemble human T1-prospermatogonia in vivo and exhibited gene expression related to spermatogenesis and diminished proliferation, a hallmark of quiescent T1 prospermatogonia. Notably, this system enabled us to visualize the dynamic and stage-specific regulation of transposable elements during human prospermatogonial specification. Together, our findings pave the way for understanding and reconstructing human male germline development in vitro.

Sign in / Sign up

Export Citation Format

Share Document