Alpha-lipoic acid attenuates endoplasmic reticulum stress-induced insulin resistance by improving mitochondrial function in HepG2 cells

2016 ◽  
Vol 28 (10) ◽  
pp. 1441-1450 ◽  
Author(s):  
Lin Lei ◽  
Yiwei Zhu ◽  
Wenwen Gao ◽  
Xiliang Du ◽  
Min Zhang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Lipoic Acid ◽  
Mitochondrial Function ◽  
Hepg2 Cells ◽  
Alpha Lipoic Acid

scholarly journals Impaired ferritinophagy induced hepatic insulin resistance via endoplasmic reticulum stress of high fat diet mice

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Chunjie Jiang ◽  
Shanshan Zhang ◽  
Hongmei Zeng ◽  
Jingjing Liu ◽  
Dan Li ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Glucose Uptake ◽  
Iron Metabolism ◽  
High Fat Diet ◽  
Hepg2 Cells ◽  
Ammonium Citrate ◽  
Iron Level ◽  
High Fat

AbstractEmerging evidence has been revealed that high fat diet (HFD) correlate with insulin resistance (IR) which could be induced by endoplasmic reticulum stress (ERS). Recently, obesity or HFD induced nonalcoholic fatty liver disease (NAFLD) could promote alteration of iron metabolism. Disorder of iron metabolism have been linked to unnormal metabolism of glucose and lipid. Herein, we investigated the effect of impaired iron homeostasis on hepatic IR, focusing on ferritinophagy. Male C57/6J mice were administered with HFD (60% energy from fat) or LFD (10% energy from fat) for 10 weeks (n = 10), and Palmitic acid (PA)-insulin treated HepG2 cells were also established. Hepatic IR as evidenced by increased hepatic steatosis and decreased of p-AKT (48%, p < 0.0005), p-GSK-3β (34%, p < 0.05) in the liver of HFD mice. In addition, decreased iron level and expression NCOA4, as well as increased up-regulation of IRE1α and EIF2α were observed in HFD liver. By using desferrioxamine (DFO) and ferric ammonium citrate (FAC), we examined iron level on IRE1α and EIF2α. And glucose uptake assay shown that FAC supplementation, and ERS inhibitors of 4-PBA and STF could improve the glucose uptake of HepG2 cells in the presence of PA. Furthermore, we evaluated the glucose uptake of HepG2 cells incubated with adenovirus which mediated overexpression of NCOA4, FAC, 4-PBA (ERS inhibitor) or STF (IRE1 inhibitor). Taken together, deficiency of iron induced by impaired ferritinophagy induced hepatic IR, partly by aggravating hepatic ERS, especially IRE1 signal pathway in vivo and vitro. These findings provide evidence and new insight for therapeutic strategy of iron deficiency in NAFLD.

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