Ursolic acid induces ER stress response to activate ASK1–JNK signaling and induce apoptosis in human bladder cancer T24 cells

2013 ◽  
Vol 25 (1) ◽  
pp. 206-213 ◽  
Author(s):  
Qing-you Zheng ◽  
Ping-ping Li ◽  
Feng-suo Jin ◽  
Chen Yao ◽  
Guo-hui Zhang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Stress Response ◽  
Er Stress ◽  
Ursolic Acid ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Jnk Signaling ◽  
Er Stress Response ◽  
T24 Cells

scholarly journals Licochalcone A-Induced Human Bladder Cancer T24 Cells Apoptosis Triggered by Mitochondria Dysfunction and Endoplasmic Reticulum Stress

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Xuan Yuan ◽  
Defang Li ◽  
Hong Zhao ◽  
Jiangtao Jiang ◽  
Penglong Wang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Parp Cleavage ◽  
Ros Generation ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Licochalcone A ◽  
T24 Cells ◽  
Stress Dependent

Licochalcone A (LCA), a licorice chalconoid, is considered to be a bioactive agent with chemopreventive potential. This study investigated the mechanisms involved in LCA-induced apoptosis in human bladder cancer T24 cells. LCA significantly inhibited cells proliferation, increased reactive oxygen species (ROS) levels, and caused T24 cells apoptosis. Moreover, LCA induced mitochondrial dysfunction, caspase-3 activation, and poly-ADP-ribose polymerase (PARP) cleavage, which displayed features of mitochondria-dependent apoptotic signals. Besides, exposure of T24 cells to LCA triggered endoplasmic reticulum (ER) stress; as indicated by the enhancement in 78 kDa glucose-regulated protein (GRP 78), growth arrest and DNA damage-inducible gene 153/C/EBP homology protein (GADD153/CHOP) expression, ER stress-dependent apoptosis is caused by the activation of ER-specific caspase-12. All the findings from our study suggest that LCA initiates mitochondrial ROS generation and induces oxidative stress that consequently causes T24 cell apoptosis via the mitochondria-dependent and the ER stress-triggered signaling pathways.

Alterations in the extracellular catabolism of nucleotides are involved in the antiproliferative effect of quercetin in human bladder cancer T24 cells

2013 ◽  
Vol 31 (7) ◽  
pp. 1204-1211 ◽  
Author(s):  
Liliana Rockenbach ◽  
Luci Bavaresco ◽  
Patrícia Fernandes Farias ◽  
Angélica Regina Cappellari ◽  
Carlos Henrique Barrios ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Antiproliferative Effect ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
T24 Cells

scholarly journals Chemosensitizing effect of maitake mushroom extract on carmustine cytotoxicity in human bladder cancer cells

2020 ◽  
Vol 8 (9) ◽  
pp. 3154
Author(s):  
Joel Hillelsohn ◽  
Michael Stern ◽  
Mina Iskander ◽  
Muhammad Choudhury ◽  
Sensuke Konno
Keyword(s):  
Oxidative Stress ◽  
Bladder Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Option ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Mushroom Extract ◽  
T24 Cells

Background: Despite several therapeutic options available for bladder cancer, the outcomes are less satisfactory.  To find a more effective modality, we were interested in the bioactive mushroom extract, PDF, which has been shown to sensitize certain anticancer drugs.  Accordingly, we investigated if cytotoxic effects of several anticancer drugs used on bladder cancer patients could be enhanced with PDF in vitro.Methods: Human bladder cancer T24 cells were treated with four anticancer drugs, carmustine (BCNU), 5-fluorouracil (5FU), cisplatin (CPL), and doxorubicin (DOX) alone, their combinations, or in combination with PDF, and cell viability was determined.  To explore the anticancer mechanism, the status of glyoxalase I (Gly-I), an enzyme involved in the drug resistance of cancer cells, and oxidative stress that can cause severe cellular injury/damage was also assessed.Results: BCNU and 5FU alone resulted in a >50% reduction in cell viability but CPL and DOX had no such effects.  Only a combination of BCNU and PDF led to a drastic (~90%) cell viability reduction, accompanied by inactivation of Gly-I and an increase in oxidative stress.  However, any combinations of other drugs and PDF had little effects on cell viability, Gly-I activity, or severity of oxidative stress.Conclusions: This study shows that anticancer activity of BCNU is significantly potentiated with PDF in T24 cells.  This is rather attributed to inactivated Gly-I and increased oxidative stress.  Therefore, PDF appears to have a chemosensitizing effect capable of enhancing BCNU cytotoxicity, which may offer an alternative, improved therapeutic option for bladder cancer.

scholarly journals Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

2019 ◽  
Vol 13 (4) ◽  
pp. 324-333 ◽  
Author(s):  
Sung Ok Kim ◽  
Hee-Jae Cha ◽  
Cheol Park ◽  
Hyesook Lee ◽  
Su Hyun Hong ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Human Bladder Cancer ◽  
Akt Signaling Pathway ◽  
Human Bladder ◽  
T24 Cells ◽  
Dependent Inhibition

scholarly journals MicroRNA-195-5p suppresses glucose uptake and proliferation of human bladder cancer T24 cells by regulating GLUT3 expression

FEBS Letters ◽  
2012 ◽  
Vol 586 (4) ◽  
pp. 392-397 ◽  
Author(s):  
Xiang Fei ◽  
Manlong Qi ◽  
Bin Wu ◽  
Yongsheng Song ◽  
Yueping Wang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Glucose Uptake ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
T24 Cells
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