Effect of orexin-A on phagocytic activity of peritoneal macrophage in starved rats

2011 ◽  
Vol 271 (1) ◽  
pp. 85-88 ◽  
Author(s):  
V. Nimet İzgüt-Uysal ◽  
Burcu Gemici ◽  
Ruken Tan
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Orexin A

Effect of Lectins on Mouse Peritoneal Macrophage Phagocytic Activity

1994 ◽  
Vol 23 (6-7) ◽  
pp. 429-436 ◽  
Author(s):  
Guadalupe Maldonado ◽  
Flor Porras ◽  
Leonor Fernández ◽  
Lorena Vázquez ◽  
Edgar Zenteno
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Mouse Peritoneal Macrophage

Acidification Enhances Peritoneal Macrophage Phagocytic Activity

2008 ◽  
Vol 147 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Julia E. Grabowski ◽  
Virginia L. Vega ◽  
Mark A. Talamini ◽  
Antonio De Maio
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity

Effect of praziquantel and liposome-incorporated praziquantel on peritoneal macrophage activation in mice infected with Mesocestoides corti tetrathyridia (Cestoda)

Parasitology ◽  
1997 ◽  
Vol 114 (5) ◽  
pp. 475-482 ◽  
Author(s):  
G. HRČKOVA ◽  
S. VELEBNY
Keyword(s):  
Peritoneal Macrophage ◽  
Peritoneal Cavity ◽  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Peritoneal Macrophages ◽  
Drug Formulation ◽  
Superoxide Anions ◽  
Effector Functions ◽  
Mouse Model System

The activation of peritoneal macrophage effector functions after therapy with free PZQ and PZQ incorporated in liposomes (lip.PZQ) was studied in the Mesocestoides corti–mouse model system. Each drug formulation was administered to an infected group of mice in 6 daily doses from day 14 p.i. Phagocytic activity of macrophages increased significantly after the administration of both drug formulations, more after lip.PZQ with an earlier peak observed for PZQ (day 3) than for lip.PZQ (day 6). Empty liposomes had no significant effect. The average counts of ingested particles in phagocytosing cells were significantly higher only after lip.PZQ administration. The pattern of changes in phagocytic activity correlated with the reduction of parasite numbers in the peritoneal cavity, with the highest observed on day 6 after therapy with lip.PZQ. Phagocytosis of lip.PZQ in vivo stimulated significantly the respiratory burst in peritoneal macrophages, with the highest concentration of superoxide anions recorded on day 1 after the last dose, whereas therapy with PZQ itself did not increase this process significantly. The capacity for the respiratory burst declined in all groups with progressing infection. It is proposed that the phagocytic activity of peritoneal macrophages after therapy was stimulated indirectly as a consequence of activation of the specific immune response. The larvicidal effect of lip.PZQ on the tetrathyridia in the peritoneal cavity was synergistic with the phagocytic activity and might be the result of double action of drug and superoxide anions generated during the respiratory burst stimulated by this drug formulation.

scholarly journals Effects of purified bacterially synthesized murine multi-CSF (IL-3) on hematopoiesis in normal adult mice

Blood ◽  
1986 ◽  
Vol 68 (1) ◽  
pp. 46-57 ◽  
Author(s):  
D Metcalf ◽  
CG Begley ◽  
GR Johnson ◽  
NA Nicola ◽  
AF Lopez ◽  
...  
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Normal Adult ◽  
Blood Eosinophil ◽  
Adult Mice ◽  
Mesenteric Lymph ◽  
C57bl Mice ◽  
Stimulating Factor

Normal adult C57BL, BALB/c, and C3H/HeJ mice were injected intraperitoneally three times daily for up to 6 days with 102,000 U (200 ng) per injection of purified, bacterially synthesized, Multipotential colony-stimulating factor (CSF) (Interleukin-3) (rMulti- CSF) and compared with control mice injected with serum/saline with or without added endotoxin (1 ng/mL). Mice injected with rMulti-CSF exhibited tenfold rises in blood eosinophil and twofold to threefold rises in neutrophil and monocyte levels. The spleens from mice injected with rMulti-CSF showed a 50% increase in weight, elevated levels of maturing granulocytes, eosinophils, nucleated erythroid cells and megakaryocytes, and up to 100-fold rises in mast cells. Progenitor cell frequencies in the spleen were elevated sixfold to 18-fold. No significant changes were observed in the marrow. Sixfold to 15-fold rises were observed in peritoneal cell populations of mice injected with rMulti-CSF with evidence of increased peritoneal macrophage phagocytic activity. Livers of C57BL mice, but not of the other strains, exhibited increased numbers of infiltrating hematopoietic cells whereas rises in mast cell numbers were observed in the mesenteric lymph node, skin, and gut in BALB/c and C3H/HeJ mice. Endotoxin was excluded as being responsible for the observed changes except possibly those involving peritoneal macrophage phagocytic activity. The results indicate that the injection of normal mice with rMulti-CSF significantly stimulates the same types of hematopoietic populations as are stimulated in vitro by Multi-CSF and indicate that this and other CSFs should be useful in stimulating hematopoietic repopulation and functional activity in vivo.

scholarly journals Effects of purified bacterially synthesized murine multi-CSF (IL-3) on hematopoiesis in normal adult mice

Blood ◽  
1986 ◽  
Vol 68 (1) ◽  
pp. 46-57 ◽  
Author(s):  
D Metcalf ◽  
CG Begley ◽  
GR Johnson ◽  
NA Nicola ◽  
AF Lopez ◽  
...  
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Normal Adult ◽  
Blood Eosinophil ◽  
Adult Mice ◽  
Mesenteric Lymph ◽  
C57bl Mice ◽  
Stimulating Factor

Abstract Normal adult C57BL, BALB/c, and C3H/HeJ mice were injected intraperitoneally three times daily for up to 6 days with 102,000 U (200 ng) per injection of purified, bacterially synthesized, Multipotential colony-stimulating factor (CSF) (Interleukin-3) (rMulti- CSF) and compared with control mice injected with serum/saline with or without added endotoxin (1 ng/mL). Mice injected with rMulti-CSF exhibited tenfold rises in blood eosinophil and twofold to threefold rises in neutrophil and monocyte levels. The spleens from mice injected with rMulti-CSF showed a 50% increase in weight, elevated levels of maturing granulocytes, eosinophils, nucleated erythroid cells and megakaryocytes, and up to 100-fold rises in mast cells. Progenitor cell frequencies in the spleen were elevated sixfold to 18-fold. No significant changes were observed in the marrow. Sixfold to 15-fold rises were observed in peritoneal cell populations of mice injected with rMulti-CSF with evidence of increased peritoneal macrophage phagocytic activity. Livers of C57BL mice, but not of the other strains, exhibited increased numbers of infiltrating hematopoietic cells whereas rises in mast cell numbers were observed in the mesenteric lymph node, skin, and gut in BALB/c and C3H/HeJ mice. Endotoxin was excluded as being responsible for the observed changes except possibly those involving peritoneal macrophage phagocytic activity. The results indicate that the injection of normal mice with rMulti-CSF significantly stimulates the same types of hematopoietic populations as are stimulated in vitro by Multi-CSF and indicate that this and other CSFs should be useful in stimulating hematopoietic repopulation and functional activity in vivo.

scholarly journals Phagocytic Activity of Ethyl Alcohol Fraction of Deer Antler in Murine Peritoneal Macrophage.

1999 ◽  
Vol 22 (9) ◽  
pp. 932-935 ◽  
Author(s):  
Jeong-Sook SUH ◽  
Jae-Soon EUN ◽  
June-No SO ◽  
Jeong-Taeg SEO ◽  
Gil-Ja JHON
Keyword(s):  
Peritoneal Macrophage ◽  
Ethyl Alcohol ◽  
Phagocytic Activity ◽  
Deer Antler ◽  
Murine Peritoneal Macrophage ◽  
Alcohol Fraction

Immunotoxicity Evaluation of Resorcinol Bis-Diphenylphosphate (Fyrolflex RDP) in B6C3F1 Mice

2000 ◽  
Vol 19 (4) ◽  
pp. 265-275 ◽  
Author(s):  
R. L. Sherwood ◽  
R. V. House ◽  
H. V. Ratajczak ◽  
R. I. Freudenthal ◽  
R. T. Henrich
Keyword(s):  
Body Weight ◽  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Nk Cell ◽  
Cell Activity ◽  
Peritoneal Cell ◽  
Host Susceptibility ◽  
Nk Cell Activity ◽  
Cell Numbers ◽  
Lymphocyte Blastogenesis

RDP was administered daily to groups of female B6C3F1 mice by oral gavage for 28 days at dose levels of 500, 1500, or 5000 mg/kg/day body weight (BW). Control animals were sham dosed. Animals were euthanized 1 day following final exposure or following a 60-day recovery period. End point observations included necropsy and histopathology, spleen and thymus weights and cellularity, peritoneal cell numbers and differential cell analysis, splenic natural killer (NK) cell activity, lymphocyte blastogenesis, antibody-forming cell (AFC) response, peritoneal macrophage phagocytic activity, and host susceptibility to Listeria monocytogenes infection. In-life observations included weekly clinical examinations and body weights. No adverse clinical signs were noted and the only significant finding was an increase in body weight in high dose mice on week 4 as compared to sham controls. Small changes in body weight gain were seen in all dose groups as compared to sham controls after completion of dosing on weeks 5 and 6 and in mid-dose mice on weeks 7 and 12. No significant differences were found in spleen and thymus weights or in cellularity. No changes were found in peritoneal cell numbers or cell types, peritoneal macrophage phagocytic activity, or host susceptibility to infection. Likewise, splenic NK cell activity, lymphocyte blastogenesis, and AFC function were also unaffected. No histopathologic changes were observed. All animals that received positive-control substances demonstrated a significant effect in the respective tests. In conclusion, a standardized and validated battery of immune function tests demonstrated no immunotoxicity following daily oral exposure to up to 5000 mg/kg BW of RDP for 28 days.

Ozone reduces murine alveolar and peritoneal macrophage phagocytosis: the role of prostanoids

1991 ◽  
Vol 261 (4) ◽  
pp. L277-L282 ◽  
Author(s):  
B. J. Canning ◽  
R. R. Hmieleski ◽  
E. W. Spannhake ◽  
G. J. Jakab
Keyword(s):  
Bronchoalveolar Lavage ◽  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Peritoneal Macrophages ◽  
Ozone Exposure ◽  
Cyclooxygenase Inhibitors ◽  
Macrophage Phagocytosis

Continuous ozone exposure (0.5 ppm, 1–14 days) reduced the phagocytic activity of murine alveolar and peritoneal macrophages. The response of peritoneal macrophages to ozone was virtually indistinguishable from the response of alveolar macrophages. When added exogenously, prostaglandin E2 (PGE2) inhibited alveolar and peritoneal macrophage phagocytosis. To test the hypothesis that prostanoids mediated the effects of ozone on macrophages, PGE levels of bronchoalveolar lavage fluid (BALF) and the phagocytic activity of macrophages from ozone-exposed mice pretreated with cyclooxygenase inhibitors were measured. PGE levels in BALF were increased following ozone exposure, with high levels of PGE associated with large decreases in phagocytic activity. Pretreatment with indomethacin and d-naproxen completely inhibited ozone-induced increases in PGE recovered by BAL and the suppression of peritoneal macrophage phagocytic activity. The inactive enantiomer of naproxen, l-naproxen, was without effect. Indomethacin partially inhibited ozone-induced suppression of alveolar macrophage phagocytic activity. These observations suggest that prostanoids play a key role in the response to ozone.

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