Establishment and characterization of SV40 large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation

2007 ◽  
Vol 31 (1) ◽  
pp. 57-64 ◽  
Author(s):  
T TAKENOUCHI ◽  
Y IWAMARU ◽  
M SATO ◽  
T YOKOYAMA ◽  
M SHINAGAWA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bovine Brain ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
Immortalized Cell Lines ◽  
Fetal Bovine ◽  
Immortalized Cell ◽  
Brain Tissues

Xeroderma Pigmentosum Variant: Generation and Characterization of Fibroblastic Cell Lines Transformed with SV40 Large T Antigen

1995 ◽  
Vol 217 (1) ◽  
pp. 100-108 ◽  
Author(s):  
Sharon A. King ◽  
Sandra J. Wilson ◽  
Rosann A. Farber ◽  
William K. Kaufmann ◽  
Marila Cordeiro-Stone
Keyword(s):  
Cell Lines ◽  
Xeroderma Pigmentosum ◽  
T Antigen ◽  
Large T Antigen ◽  
Fibroblastic Cell ◽  
Sv40 Large T Antigen

Characterization of immortalized human umbilical and iliac vein endothelial cell lines after transfection with SV40 large T-antigen

2000 ◽  
Vol 11 (1) ◽  
pp. 15-25 ◽  
Author(s):  
E. B. M. van Leeuwen ◽  
R. Veenstra ◽  
R. van Wijk ◽  
G. Molema ◽  
A. Hoekstra ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Lines ◽  
Iliac Vein ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen

Characterization of immortalized human umbilical and iliac vein endothelial cell lines after transfection with SV40 large T-antigen

2000 ◽  
Vol 11 (1) ◽  
pp. 15-25 ◽  
Author(s):  
E. B. M. van Leeuwen ◽  
R. Veenstra ◽  
R. van Wijk ◽  
G. Molema ◽  
A. Hoekstra ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Lines ◽  
Iliac Vein ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen

Establishment and characterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice

2001 ◽  
Vol 81 (4) ◽  
pp. 571-582 ◽  
Author(s):  
E. Kitaoka ◽  
K. Satomura ◽  
E. Hayashi ◽  
K. Yamanouchi ◽  
S. Tobiume ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Lines ◽  
Costal Cartilage ◽  
T Antigen ◽  
Large T Antigen ◽  
Sv40 Large T Antigen

Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens

1986 ◽  
Vol 6 (4) ◽  
pp. 1204-1217
Author(s):  
P S Jat ◽  
C L Cepko ◽  
R C Mulligan ◽  
P A Sharp
Keyword(s):  
Cell Lines ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Vector System ◽  
Large T Antigen ◽  
T Antigens ◽  
Sv40 Large T Antigen ◽  
Polyomavirus Middle T Antigen ◽  
Middle T Antigen

We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.

Growth Properties of Neural Cell Lines Immortalized with the Tsa58 Allele of Sv40 Large T Antigen

1997 ◽  
Vol 6 (3) ◽  
pp. 231-238 ◽  
Author(s):  
M.E. Truckenmiller ◽  
Ora Dillon-Carter ◽  
Carlo Tornatore ◽  
Henrietta Kulaga ◽  
Hidetoshi Takashima ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Line ◽  
Cell Lines ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Large T Antigen ◽  
Wild Type ◽  
Sv40 Large T Antigen ◽  
Growth Properties

In vitro growth properties of three CNS-derived cell lines were compared under a variety of culture conditions. The M213-20 and J30a cell lines were each derived from embryonic CNS culture with the temperature-sensitive (ts) allele of SV40 large T antigen, tsA58, while the A7 cell line was immortalized using wild-type SV40 large T antigen. Cells immortalized with tsA58 SV40 large T proliferate at the permissive temperature, 33° C, while growth is expected to be suppressed at the nonpermissive temperature, 39.5°C. Both the M213-20 and J30a cell lines were capable of proliferating at 39.5°C continuously for up to 6 mo. All three cell lines showed no appreciable differences in growth rates related to temperature over a 7-day period in either serum-containing or defined serum-free media. The percentage of cells in S-phase of the cell cycle did not decrease or was elevated at 39.5°C for all three cell lines. After 3 wk at 39.5°C, the three cell lines also showed positive immunostaining using two monoclonal antibodies reacting with different epitopes of SV40 large T antigen. Double strand DNA sequence analyses of a 300 base pair (bp) fragment of the large T gene from each cell line, which included the ts locus, revealed mutations in both the J30a and M213-20 cell lines. The J30a cell line ts mutation had reverted to wild type, and two additional loci with bp substitutions with predicted amino acid changes were also found. While the ts mutation of the M213-20 cells was retained, an additional bp substitution with a predicted amino acid change was found. The A7 cell line sequence was identical to the reference wild-type sequence. These findings suggest that (a) nucleic acid sequences in the temperature-sensitive region of the tsA58 allele of SV40 large T are not necessarily stable, and (b) temperature sensitivity of cell lines immortalized with tsA58 is not necessarily retained.

scholarly journals Differences in the Reactivity of CD4+ T-Cell Lines Generated against Free Versus Nucleosome-Bound SV40 Large T Antigen

2001 ◽  
Vol 53 (4) ◽  
pp. 372-380 ◽  
Author(s):  
G. Bredholt ◽  
O.P. Rekvig ◽  
K. Andreassen ◽  
U. Moens ◽  
T.N. Marion
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
T Antigen ◽  
Cd4 T Cell ◽  
Large T Antigen ◽  
Sv40 Large T Antigen ◽  
T Cell Lines
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