scholarly journals Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins

Cell ◽  
2020 ◽  
Vol 182 (2) ◽  
pp. 345-356.e16 ◽  
Author(s):  
Hunsang Lee ◽  
Greg L. Beilhartz ◽  
Iga Kucharska ◽  
Swetha Raman ◽  
Hong Cui ◽  
...  
Keyword(s):  
Lethal Toxin ◽  
Receptor Specificity

scholarly journals Identification of the C. sordellii lethal toxin receptor elucidates principles of receptor specificity in clostridial toxins

2019 ◽  
Author(s):  
Hunsang Lee ◽  
Greg L. Beilhartz ◽  
Iga Kucharska ◽  
Swetha Raman ◽  
Hong Cui ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Molecular Basis ◽  
Lethal Toxin ◽  
Toxic Shock ◽  
Receptor Specificity ◽  
Cellular Receptors ◽  
Tissue Targeting ◽  
Toxin Receptor ◽  
Frizzled Receptors ◽  
The Difference

AbstractClostridium sordellii lethal toxin (TcsL) is responsible for an almost invariably lethal toxic shock syndrome associated with gynecological C. sordellii infections. Here, using CRISPR/Cas9 screening, we identify semaphorins SEMA6A and SEMA6B as the cellular receptors for TcsL and demonstrate that soluble extracellular SEMA6A can protect mice from TcsL-induced edema. A 3.3 Å cryo-EM structure shows that TcsL binds SEMA6A with the same region that the highly related C. difficile TcdB toxin uses to bind structurally unrelated Frizzled receptors. Remarkably, reciprocal mutations in this evolutionarily divergent surface are sufficient to switch receptor specificity between the toxins. Our findings establish semaphorins as physiologically relevant receptors for TcsL, and reveal the molecular basis for the difference in tissue targeting and disease pathogenesis between highly related toxins.

Analysis of the Receptor Specificity of Influenza A Virus Strains Using the Method of Surface Plasmon Resonance

BIOPHYSICS ◽  
2020 ◽  
Vol 65 (2) ◽  
pp. 222-224
Author(s):  
G. S. Onkhonova ◽  
P. Yu. Torzhkova ◽  
V. Yu. Marchenko ◽  
S. V. Svyatchenko ◽  
A. S. Gudymo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Influenza A Virus ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Influenza A ◽  
Receptor Specificity ◽  
Virus Strains
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