scholarly journals A Viral Movement Protein Prevents Spread of the Gene Silencing Signal in Nicotiana benthamiana

Cell ◽  
2016 ◽  
Vol 166 (3) ◽  
pp. 780 ◽  
Author(s):  
Olivier Voinnet ◽  
Carsten Lederer ◽  
David C. Baulcombe
Keyword(s):  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
Movement Protein ◽  
Viral Movement Protein ◽  
Viral Movement

scholarly journals A Viral Movement Protein Prevents Spread of the Gene Silencing Signal in Nicotiana benthamiana

Cell ◽  
2000 ◽  
Vol 103 (1) ◽  
pp. 157-167 ◽  
Author(s):  
Olivier Voinnet ◽  
Carsten Lederer ◽  
David C Baulcombe
Keyword(s):  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
Movement Protein ◽  
Viral Movement Protein ◽  
Viral Movement

scholarly journals Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

2002 ◽  
Vol 14 (9) ◽  
pp. 2071-2083 ◽  
Author(s):  
Asuka Itaya ◽  
Fengshan Ma ◽  
Yijun Qi ◽  
Yoshie Matsuda ◽  
Yali Zhu ◽  
...  
Keyword(s):  
Movement Protein ◽  
Isolated Cells ◽  
Viral Movement Protein ◽  
Protein Traffic ◽  
Viral Movement

GST fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies

2001 ◽  
Vol 91 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Mei-Yun Zhang ◽  
Stefan Schillberg ◽  
Sabine Zimmermann ◽  
Yu-Cai Liao ◽  
Gudrun Breuer ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Movement Protein ◽  
False Positives ◽  
Single Chain ◽  
Viral Movement Protein ◽  
Single Chain Antibodies ◽  
Viral Movement ◽  
Selection Of ◽  
Gst Fusion Proteins

scholarly journals Downregulation of the NbNACa1 Gene Encoding a Movement-Protein-Interacting Protein Reduces Cell-to-Cell Movement of Brome mosaic virus in Nicotiana benthamiana

2007 ◽  
Vol 20 (6) ◽  
pp. 671-681 ◽  
Author(s):  
Masanori Kaido ◽  
Yosuke Inoue ◽  
Yoshika Takeda ◽  
Kazuhiko Sugiyama ◽  
Atsushi Takeda ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Fluorescent Protein ◽  
Movement Protein ◽  
Sequence Similarity ◽  
Microprojectile Bombardment ◽  
Cell Movement ◽  
Brome Mosaic Virus ◽  
Viral Movement

The 3a movement protein (MP) plays a central role in the movement of the RNA plant virus, Brome mosaic virus (BMV). To identify host factor genes involved in viral movement, a cDNA library of Nicotiana benthamiana, a systemic host for BMV, was screened with far-Western blotting using a recombinant BMV MP as probe. One positive clone encoded a protein with sequence similarity to the α chain of nascent-polypeptide-associated complex from various organisms, which is proposed to contribute to the fidelity of translocation of newly synthesized proteins. The orthologous gene from N. benthamiana was designated NbNACa1. The binding of NbNACa1 to BMV MP was confirmed in vivo with an agroinfiltration-immunoprecipitation assay. To investigate the involvement of NbNACa1 in BMV multiplication, NbNACa1-silenced (GSNAC) transgenic N. benthamiana plants were produced. Downregulation of NbNACa1 expression reduced virus accumulation in inoculated leaves but not in protoplasts. A microprojectile bombardment assay to monitor BMV-MP-assisted viral movement demonstrated reduced virus spread in GSNAC plants. The localization to the cell wall of BMV MP fused to green fluorescent protein was delayed in GSNAC plants. From these results, we propose that NbNACa1 is involved in BMV cell-to-cell movement through the regulation of BMV MP localization to the plasmodesmata.

scholarly journals The Unfolded Protein Response Is Triggered by a Plant Viral Movement Protein

2011 ◽  
Vol 156 (2) ◽  
pp. 741-755 ◽  
Author(s):  
Changming Ye ◽  
Martin B. Dickman ◽  
Steven A. Whitham ◽  
Mark Payton ◽  
Jeanmarie Verchot
Keyword(s):  
Unfolded Protein Response ◽  
Movement Protein ◽  
Viral Movement Protein ◽  
Unfolded Protein ◽  
Viral Movement ◽  
The Unfolded Protein Response ◽  
Protein Response
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