scholarly journals DICER1 Loss and Alu RNA Induce Age-Related Macular Degeneration via the NLRP3 Inflammasome and MyD88

Cell ◽  
2012 ◽  
Vol 149 (4) ◽  
pp. 847-859 ◽  
Author(s):  
Valeria Tarallo ◽  
Yoshio Hirano ◽  
Bradley D. Gelfand ◽  
Sami Dridi ◽  
Nagaraj Kerur ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Nlrp3 Inflammasome ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Alu Rna

scholarly journals DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration

Nature ◽  
2011 ◽  
Vol 471 (7338) ◽  
pp. 325-330 ◽  
Author(s):  
Hiroki Kaneko ◽  
Sami Dridi ◽  
Valeria Tarallo ◽  
Bradley D. Gelfand ◽  
Benjamin J. Fowler ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Rna Toxicity ◽  
Age Related ◽  
Alu Rna

scholarly journals Baicalin Alleviates Age-Related Macular Degeneration via miR-223/NLRP3-Regulated Pyroptosis

Pharmacology ◽  
2019 ◽  
Vol 105 (1-2) ◽  
pp. 28-38 ◽  
Author(s):  
Hong-Jing Sun ◽  
Xiu-Ming Jin ◽  
Jia Xu ◽  
Qing Xiao
Keyword(s):  
Western Blot ◽  
Macular Degeneration ◽  
Blot Analysis ◽  
Nlrp3 Inflammasome ◽  
Western Blot Analysis ◽  
Retinal Pigment ◽  
Age Related Macular Degeneration ◽  
Luciferase Reporter ◽  
Potential Candidate ◽  
Age Related

Background: Age-related macular degeneration (AMD), a major eye degenerative disease, ultimately causes irreversible vision loss. Baicalin was identified to attenuate laser-induced chorodial neovascularization, indicating a therapeutic role in AMD. However, the exact mechanisms for baicalin in AMD remain unknown. Methods: MTT assay was performed to access the suitable concentration of baicalin or Aβ for treating ARPE-19 cells. CCK-8, morphology, and flow cytometry analysis were performed to evaluate cell viability and pyroptosis of baicalin in Aβ-envoked ARPE-19 cells. Quantitative real-time polymerase chain reaction and western blot analysis were subjected to measure the correlation between miR-223 and NLRP3. Luciferase reporter assay was performed to determine their direct relationship. Western blot analysis was subjected to determine pyroptosis-related proteins. Results: Baicalin inhibited Aβ-envoked pyroptosis in ARPE-19 cells. Mechanistically, baicalin significantly induced upregulation of miR-223 and downregulation of NLRP3, thus suppressing pyroptosis triggered by NLRP3 inflammasome signaling, yet such beneficial effects were reversed by miR-223 knockdown. Additionally, MCC950, a NLRP3 inhibitor, restored anti-pyroptosis activity of baicalin under miR-223 silencing. Conclusion: Baicalin alleviates intracellular pyroptosis and viability damage resulted from Aβ inducement in human retinal pigment epithelium cells via negative crosstalk of miR-223/NLRP3 inflammasome signaling, indicating that baicalin may be considered as a potential candidate for AMD therapy.

scholarly journals DICER1 in the Pathogenesis of Age-related Macular Degeneration (AMD) - Alu RNA Accumulation versus miRNA Dysregulation

2020 ◽  
Vol 11 (4) ◽  
pp. 851 ◽  
Author(s):  
Kai Kaarniranta ◽  
Elzbieta Pawlowska ◽  
Joanna Szczepanska ◽  
Janusz Blasiak
Keyword(s):  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Alu Rna ◽  
Rna Accumulation
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