scholarly journals Mechanosensitive Hair Cell-like Cells from Embryonic and Induced Pluripotent Stem Cells

Cell ◽  
2010 ◽  
Vol 141 (4) ◽  
pp. 704-716 ◽  
Author(s):  
Kazuo Oshima ◽  
Kunyoo Shin ◽  
Marc Diensthuber ◽  
Anthony W. Peng ◽  
Anthony J. Ricci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Generation of Otic Lineages from Integration-Free Human-Induced Pluripotent Stem Cells Reprogrammed by mRNAs

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Sarah L. Boddy ◽  
Ricardo Romero-Guevara ◽  
Ae-Ri Ji ◽  
Christian Unger ◽  
Laura Corns ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Cell ◽  
Induced Pluripotent Stem Cells ◽  
Cell Lines ◽  
Hair Cells ◽  
Pluripotent Stem Cells ◽  
Genetic Alterations ◽  
Pluripotent Cell ◽  
Induced Pluripotent ◽  
Ganglion Neurons

Damage to the sensory hair cells and the spiral ganglion neurons of the cochlea leads to deafness. Induced pluripotent stem cells (iPSCs) are a promising tool to regenerate the cells in the inner ear that have been affected by pathology or have been lost. To facilitate the clinical application of iPSCs, the reprogramming process should minimize the risk of introducing undesired genetic alterations while conferring the cells the capacity to differentiate into the desired cell type. Currently, reprogramming induced by synthetic mRNAs is considered to be one of the safest ways of inducing pluripotency, as the transgenes are transiently delivered into the cells without integrating into the genome. In this study, we explore the ability of integration-free human-induced pluripotent cell lines that were reprogrammed by mRNAs, to differentiate into otic progenitors and, subsequently, into hair cell and neuronal lineages. hiPSC lines were induced to differentiate by culturing them in the presence of fibroblast growth factors 3 and 10 (FGF3 and FGF10). Progenitors were identified by quantitative microscopy, based on the coexpression of otic markers PAX8, PAX2, FOXG1, and SOX2. Otic epithelial progenitors (OEPs) and otic neuroprogenitors (ONPs) were purified and allowed to differentiate further into hair cell-like cells and neurons. Lineages were characterised by immunocytochemistry and electrophysiology. Neuronal cells showed inward Na+ (INa) currents and outward (Ik) and inward K+ (IK1) currents while hair cell-like cells had inward IK1 and outward delayed rectifier K+ currents, characteristic of developing hair cells. We conclude that human-induced pluripotent cell lines that have been reprogrammed using nonintegrating mRNAs are capable to differentiate into otic cell types.

scholarly journals Limited hair cell induction from human induced pluripotent stem cells using a simple stepwise method

2015 ◽  
Vol 599 ◽  
pp. 49-54 ◽  
Author(s):  
Hiroe Ohnishi ◽  
Desislava Skerleva ◽  
Shin-ichiro Kitajiri ◽  
Tatsunori Sakamoto ◽  
Norio Yamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Stepwise Method ◽  
Cell Induction ◽  
Induced Pluripotent

scholarly journals Effects of mouse utricle stromal tissues on hair cell induction from induced pluripotent stem cells

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Akiko Taura ◽  
Hiroe Ohnishi ◽  
Shohei Ochi ◽  
Fumi Ebisu ◽  
Takayuki Nakagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Induction ◽  
Induced Pluripotent

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent
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