Single-step microfluidic production of W/O/W double emulsions as templates for β-carotene-loaded giant liposomes formation

2019 ◽  
Vol 366 ◽  
pp. 27-32 ◽  
Author(s):  
Mariano Michelon ◽  
Yuting Huang ◽  
Lucimara Gaziola de la Torre ◽  
David A. Weitz ◽  
Rosiane Lopes Cunha
Keyword(s):  
Single Step ◽  
Double Emulsions ◽  
Β Carotene

scholarly journals Prediction and control of drop formation modes in microfluidic generation of double emulsions by single-step emulsification

2017 ◽  
Vol 505 ◽  
pp. 315-324 ◽  
Author(s):  
Seyed Ali Nabavi ◽  
Goran T. Vladisavljević ◽  
Monalie V. Bandulasena ◽  
Omid Arjmandi-Tash ◽  
Vasilije Manović
Keyword(s):  
Single Step ◽  
Drop Formation ◽  
Double Emulsions ◽  
Prediction And Control ◽  
And Control

scholarly journals PCR & Go: A Pre-installed Expression Chassis for Facile Integration of Multi-Gene Biosynthetic Pathways

2021 ◽  
Vol 8 ◽  
Author(s):  
Mingming Qi ◽  
Bei Zhang ◽  
Lihong Jiang ◽  
Saijuan Xu ◽  
Chang Dong ◽  
...  
Keyword(s):  
High Efficiency ◽  
Functional Expression ◽  
Single Step ◽  
Yeast Genome ◽  
Biosynthetic Pathways ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Plasmid Library ◽  
Yeast Chromosome ◽  
Β Carotene

The introduction of multi-gene metabolic pathways is generally the first step for the construction of microbial cell factories and plays an essential role in metabolic engineering and synthetic biology. Here, we developed a “PCR & Go” system for facile integration and assembly of multi-gene pathways into the chromosome of Saccharomyces cerevisiae. The core component of the “PCR & Go” system was an expression chassis, where eight promoter/terminator pairs were pre-installed into the yeast chromosome and PCR amplified gene fragments could be inserted directly for functional expression. In combination with the CRISPR/Cas9 system and a gRNA plasmid library, the β-carotene (three genes), zeaxanthin (four genes), and astaxanthin (five genes) biosynthetic pathways were integrated and assembled into the yeast genome with an efficiency of ~93, ~85, and 69%, respectively, using PCR amplified gene fragments with ~40 bp homology arms in a single step. Therefore, the “PCR & Go” system can be used for fast construction of yeast cell factories harboring multi-gene pathways with high efficiency and flexibility.

Single step emulsification for the generation of multi-component double emulsions

Soft Matter ◽  
2012 ◽  
Vol 8 (41) ◽  
pp. 10719 ◽  
Author(s):  
L. L. A. Adams ◽  
Thomas E. Kodger ◽  
Shin-Hyun Kim ◽  
Ho Cheng Shum ◽  
Thomas Franke ◽  
...  
Keyword(s):  
Single Step ◽  
Double Emulsions

Scalable single-step microfluidic production of single-core double emulsions with ultra-thin shells

Lab on a Chip ◽  
2015 ◽  
Vol 15 (16) ◽  
pp. 3335-3340 ◽  
Author(s):  
L. R. Arriaga ◽  
E. Amstad ◽  
D. A. Weitz
Keyword(s):  
Thin Shell ◽  
Single Step ◽  
Thin Shells ◽  
Double Emulsions ◽  
Drop Radius ◽  
Microfluidic Technique

We report a scalable single-step microfluidic technique for the production of monodisperse double emulsions with very thin shell thicknesses, of about 5% of the drop radius.

V884: Demonstration of a Novel Single-Step Percutaneous Access Sheath for Nephrostolithotomy: A Video Presentation

2005 ◽  
Vol 173 (4S) ◽  
pp. 240-240
Author(s):  
Premal J. Desai ◽  
David A. Hadley ◽  
Lincoln J. Maynes ◽  
D. Duane Baldwin
Keyword(s):  
Single Step ◽  
Video Presentation ◽  
Percutaneous Access ◽  
Access Sheath

Sustained delivery of salbutamol and beclometasone from spray-dried double emulsions

2009 ◽  
pp. 090624002829090
Author(s):  
Tristan P. Learoyd ◽  
Jane L. Burrows ◽  
Eddie French ◽  
Peter C. Seville
Keyword(s):  
Sustained Delivery ◽  
Double Emulsions ◽  
Spray Dried

Serum Retinol and β-carotene Levels and Risk Factors for Cardiovascular Disease in Morbid Obesity

2010 ◽  
Vol 80 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Gabriela Villaça Chaves ◽  
Gisele Gonçalves de Souza ◽  
Andréa Cardoso de Matos ◽  
Dra. Wilza Abrantes Peres ◽  
Silvia Elaine Pereira ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cardiovascular Disease ◽  
Metabolic Syndrome ◽  
Morbid Obesity ◽  
Serum Levels ◽  
World Health ◽  
Morbidly Obese ◽  
Serum Retinol ◽  
Significant Difference ◽  
Β Carotene

Objective: To evaluate retinol and β-carotene serum levels and their relationship with risk factors for cardiovascular disease in individuals with morbid obesity, resident in Rio de Janeiro. Methodology: Blood serum concentrations of retinol and β-carotene of 189 morbidly obese individuals were assessed. The metabolic syndrome was identified according to the criteria of the National Cholesterol Education Program (NCEP) and World Health Organization (WHO). Lipid profile, insulin resistance, basal insulin, glycemia, blood pressure, and anthropometry and their correlation with retinol and β-carotene serum levels were evaluated. Results: Metabolic syndrome diagnosis was observed in 49.0% of the sample. Within this percentage the levels of β-carotene were significantly lower when body mass index increased. Serum retinol didn't show this behavior. Serum retinol inadequacy in patients with metabolic syndrome (61.3%), according to WHO criterion, was higher (15.8%) than when the whole sample was considered (12.7%). When metabolic syndrome was diagnosed by NCEP criterion, β-carotene inadequacy was higher (42.8%) when compared to the total sample (37.5%). There was a significant difference between average β-carotene values of patients with and without metabolic syndrome (p=0.048) according to the classification of the NCEP. Lower values were found in patients with metabolic syndrome. Conclusion: Considering the vitamin A contribution in antioxidant protection, especially when risk factors for cardiovascular disease are present, it is suggested that great attention be given to morbidly obese. This could aid in prevention and treatment of cardiovascular disease, which affects a significant part of the population.

Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 25-29 ◽  
Author(s):  
Guangwen Tang
Keyword(s):  
Stable Isotope ◽  
Vitamin A ◽  
Provitamin A ◽  
Animal Origin ◽  
Plant Food ◽  
Plant Foods ◽  
A Value ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

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