scholarly journals CRISPR/Cas9 Gene editing of RyR2 in human stem cell-derived cardiomyocytes provides a novel approach in investigating dysfunctional Ca2+ signaling

Cell Calcium ◽  
2018 ◽  
Vol 73 ◽  
pp. 104-111 ◽  
Author(s):  
Hua Wei ◽  
Xiao-Hua Zhang ◽  
Cassandra Clift ◽  
Naohiro Yamaguchi ◽  
Martin Morad
Keyword(s):  
Stem Cell ◽  
Gene Editing ◽  
Human Stem Cell ◽  
Novel Approach

scholarly journals Probing the RyR2 Ca2+ and Caffeine Binding Sites by Mutagenesis in Human Stem-Cell Derived Cardiomyocytes by CRISPR/Cas9 Gene Editing

2020 ◽  
Vol 118 (3) ◽  
pp. 255a
Author(s):  
Yanli Xia ◽  
Xiaohua Zhang ◽  
Naohiro Yamaguchi ◽  
Martin Morad
Keyword(s):  
Stem Cell ◽  
Binding Sites ◽  
Gene Editing ◽  
Human Stem Cell

Human stem cell models of neurodegeneration: a novel approach to study mechanisms of disease development

2013 ◽  
Vol 127 (2) ◽  
pp. 151-173 ◽  
Author(s):  
Gunnar Hargus ◽  
Marc Ehrlich ◽  
Anna-Lena Hallmann ◽  
Tanja Kuhlmann
Keyword(s):  
Stem Cell ◽  
Disease Development ◽  
Human Stem Cell ◽  
Cell Models ◽  
Novel Approach ◽  
Stem Cell Models

scholarly journals Enhanced maturation of human stem cell derived interneurons by mTOR activation

2019 ◽  
Author(s):  
Jianhua Chu ◽  
Megan L. Fitzgerald ◽  
Neha Sehgal ◽  
William Manley ◽  
Shane Fitzgerald ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mtor Inhibitor ◽  
Directed Differentiation ◽  
Human Stem Cell ◽  
Cortical Interneuron ◽  
Novel Approach ◽  
Human Neurons ◽  
Cell Recording ◽  
Rat Neocortex ◽  
Line Following

SummaryThe use of stem cell derived neurons for cell-based therapies is limited by a protracted maturation. We present a novel approach for accelerating the post-mitotic maturation of human stem cell derived interneurons via the activation of mTOR signaling. Lox sites were placed within PTEN, a key mTOR inhibitor, in a cortical interneuron (CIn) reporter line. Following directed differentiation and purification by FACS, the CIns were exposed to Cre-expressing lentivirus, then transplanted into mouse neocortex or plated onto cultured rat neocortex. Input synaptogenesis and dendritogenesis was greatly enhanced in the PTEN-deleted CIns. Whole-cell recording of the PTEN-deleted CIns in slices of transplanted neocortex revealed multiple indices of enhanced maturation. Finally, we observed similar effects using transient, doxycycline-inducible activation of AKT. We thus present an inducible, reversible approach for accelerating the maturation of human stem cell derived CIns, and to study the influences of this disease-related signaling system in human neurons.

An Update on Human Stem Cell-Based Therapy in Parkinson';s Disease

2016 ◽  
Vol 11 (7) ◽  
pp. 561-568 ◽  
Author(s):  
Clara González ◽  
Sonia Bonilla ◽  
Ana Isabel Flores ◽  
Eva Cano ◽  
Isabel Liste
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Cell Based Therapy
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