scholarly journals The involvement of calcium carriers and of the vacuole in the glucose-induced calcium signaling and activation of the plasma membrane H+-ATPase in Saccharomyces cerevisiae cells

Cell Calcium ◽  
2012 ◽  
Vol 51 (1) ◽  
pp. 72-81 ◽  
Author(s):  
L.E.M. Bouillet ◽  
A.S. Cardoso ◽  
E. Perovano ◽  
R.R. Pereira ◽  
E.M.C. Ribeiro ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Calcium Signaling

scholarly journals Lpx1p links glucose-induced calcium signaling and plasma membrane H+-ATPase activation in Saccharomyces cerevisiae cells

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Diogo Dias Castanheira ◽  
Eduardo Perovano Santana ◽  
Fernanda Godoy-Santos ◽  
Raphael Hermano Santos Diniz ◽  
Fábio Faria-Oliveira ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Calcium Signaling

scholarly journals Carbonyl cyanide m-chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H+-ATPase in the yeast Saccharomyces cerevisiae

2008 ◽  
Vol 8 (4) ◽  
pp. 622-630 ◽  
Author(s):  
Michele B.P. Pereira ◽  
Renata Tisi ◽  
Luciano G. Fietto ◽  
Anamaria S. Cardoso ◽  
Mônica M. França ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Calcium Signaling ◽  
Yeast Saccharomyces Cerevisiae ◽  
Carbonyl Cyanide

Calcium signaling and sugar-induced activation of plasma membrane H+-ATPase in Saccharomyces cerevisiae cells

2006 ◽  
Vol 343 (4) ◽  
pp. 1234-1243 ◽  
Author(s):  
Maria José M. Trópia ◽  
Anamaria S. Cardoso ◽  
Renata Tisi ◽  
Luciano G. Fietto ◽  
Juliana L.R. Fietto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Calcium Signaling

scholarly journals Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein

1999 ◽  
Vol 339 (2) ◽  
pp. 299-307 ◽  
Author(s):  
Arthur L. KRUCKEBERG ◽  
Ling YE ◽  
Jan A. BERDEN ◽  
Karel van DAM
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Green Fluorescent Protein ◽  
Glucose Transport ◽  
Cellular Localization ◽  
Fluorescent Protein ◽  
Hexose Transporter ◽  
High Concentrations ◽  
Green Fluorescent

The Hxt2 glucose transport protein of Saccharomyces cerevisiae was genetically fused at its C-terminus with the green fluorescent protein (GFP). The Hxt2-GFP fusion protein is a functional hexose transporter: it restored growth on glucose to a strain bearing null mutations in the hexose transporter genes GAL2 and HXT1 to HXT7. Furthermore, its glucose transport activity in this null strain was not markedly different from that of the wild-type Hxt2 protein. We calculated from the fluorescence level and transport kinetics that induced cells had 1.4×105 Hxt2-GFP molecules per cell, and that the catalytic-centre activity of the Hxt2-GFP molecule in vivo is 53 s-1 at 30 °C. Expression of Hxt2-GFP was induced by growth at low concentrations of glucose. Under inducing conditions the Hxt2-GFP fluorescence was localized to the plasma membrane. In a strain impaired in the fusion of secretory vesicles with the plasma membrane, the fluorescence accumulated in the cytoplasm. When induced cells were treated with high concentrations of glucose, the fluorescence was redistributed to the vacuole within 4 h. When endocytosis was genetically blocked, the fluorescence remained in the plasma membrane after treatment with high concentrations of glucose.

scholarly journals High yield expression of the Neurospora crassa plasma membrane H(+)-ATPase in Saccharomyces cerevisiae

1994 ◽  
Vol 269 (26) ◽  
pp. 17705-17712
Author(s):  
S.K. Mahanty ◽  
U.S. Rao ◽  
R.A. Nicholas ◽  
G.A. Scarborough
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Neurospora Crassa ◽  
High Yield

scholarly journals Transport to the plasma membrane is regulated differently early and late in the cell cycle in Saccharomyces cerevisiae

2011 ◽  
Vol 124 (7) ◽  
pp. 1055-1066 ◽  
Author(s):  
B. Zanolari ◽  
U. Rockenbauch ◽  
M. Trautwein ◽  
L. Clay ◽  
Y. Barral ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Plasma Membrane
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